...Atsuhiro Adachi; Fumi Kano; Takashi Tsuboi; Morihisa Fujita; Yusuke Maeda; Masayuki Murata Glycogen synthase kinase β (GSK3β) phosphorylates many substrates in mammalian cells, and functions in many physiological processes. We observed that GSK3β knockdown by siRNA perturbed both Golgi morphology...

... on MAP1B, and selected for further study a putative primed GSK3β site and compared it with two nonprimed GSK3β sites that we had previously characterised. We raised a panel of phosphospecific antibodies to these sites on MAP1B and used it to assess the distribution of phosphorylated MAP1B in the developing...

... of GSK3β gave similar results to those of Sfrp1 , and a phosphorylation-dependent inhibition of GSK3β activity followed SFRP1 treatment of retina cells. Furthermore, retroviral mediated expression of a dominant-negative form of GSK3β induced a retina phenotype similar to that observed after Sfrp1...

...Robert G. Goold; Phillip R. Gordon-Weeks In recent studies we have demonstrated that glycogen synthase kinase 3β (GSK3β) and its substrate microtubule-associated protein 1B (MAP1B) regulate the microtubule cytoskeleton during axon outgrowth. To further examine the role GSK3β plays in axon outgrowth...

...Robert G. Goold; Rebecca Owen; Phillip R. Gordon-Weeks ABSTRACT We have recently shown that glycogen synthase kinase 3β (GSK3β) phosphorylates the microtubule-associated protein (MAP) 1B in an in vitro kinase assay and in cultured cerebellar granule cells. Mapping studies identified a region...