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Keywords: GFP
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Journal Articles
J Cell Sci (2015) 128 (23): 4328–4340.
Published: 1 December 2015
..., with a precision unsurpassed in other systems. PIP 2 Pleckstrin homology domain Phototransduction Fluorescent probes GFP Voltage-sensitive phosphatase Phototransduction in Drosophila is mediated by a G-protein-coupled phospholipase C (PLC) cascade and is an influential model...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (17): 2877–2890.
Published: 1 September 2011
.... Collectively, these methods present a paradigm shift towards the molecular-level investigation of disease and therapy in animal models of disease. * Author for correspondence ( k.anderson@beatson.gla.ac.uk ) © 2011. 2011 In vivo imaging Intravital imaging FRAP FRET FLIM GFP SHG...
Journal Articles
J Cell Sci (2008) 121 (16): 2768–2781.
Published: 15 August 2008
... and overexpression phenotypes. Further analysis of two of these proteins, Rab18 and Rab43, strongly indicated roles in ER-Golgi trafficking. Rab43-T32N redistributed Golgi elements to ER exit sites without blocking trafficking of the secretory marker VSVG-GFP from ER to cell surface. Wild-type Rab43 redistributes...
Includes: Supplementary data
Journal Articles
J Cell Sci (2008) 121 (14): 2339–2349.
Published: 15 July 2008
...-mail: D.S.Verbeek@medgen.umcg.nl ) 22 4 2008 © The Company of Biologists Limited 2008 2008 Spinocerebellar ataxia Protein kinase C gamma GFP FLIM FRET The protein kinase C (PKC) family encompasses a group of serine/threonine kinases that have important roles in many...
Journal Articles
J Cell Sci (2007) 120 (24): 4302–4309.
Published: 15 December 2007
.... If this is the case, the cell membrane underneath MiDASes must be attached to the substratum. To assess this possibility, myosin-null cells were observed by interference reflection microscopy (IRM), which is generally used to detect the adhesion sites of cells. Fig. 6A shows dual images of GFP-actin and IRM, which...
Includes: Multimedia, Supplementary data
Journal Articles
In collection:
Lipid Biology
J Cell Sci (2006) 119 (24): 5087–5097.
Published: 15 December 2006
... cells, GFP-tagged versions of the Arabidopsis thaliana heterotrimeric G protein subunits were constructed. Mutational analysis showed that the Arabidopsis Gα subunit, GPα1, contains two lipidation motifs that were essential for plasma membrane localization. The Arabidopsis Gβ subunit, AGβ1, and the Gγ...
Includes: Supplementary data
Journal Articles
J Cell Sci (2006) 119 (15): 3193–3205.
Published: 1 August 2006
...Pankaj Dhonukshe; Norbert Vischer; Theodorus W. J. Gadella, Jr The spindle occupies a central position in cell division as it builds up the chromosome-separating machine. Here we analysed the dynamics of spindle formation in acentrosomal plant cells by visualizing microtubules labelled with GFP-EB1...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2005) 118 (20): 4879–4888.
Published: 15 October 2005
...Péter Várnai; Tzvetanka Bondeva; Péter Tamás; Balázs Tóth; László Buday; László Hunyady; Tamas Balla Several pleckstrin-homology (PH) domains with the ability to bind phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5) P 3 , PIP 3 ] were expressed as green fluorescent protein (GFP...
Journal Articles
J Cell Sci (2004) 117 (25): 6095–6104.
Published: 1 December 2004
... posttranslational modification. Moreover, in transfected HeLa cells, chimeric GFP containing a nuclear localization signal and a C-terminal CaaX motif of N-Ras induces intranuclear membrane stacks that resemble those induced by lamins and ER-like cisternae that are induced in the cytoplasm upon increased synthesis...
Journal Articles
J Cell Sci (2004) 117 (18): 4113–4125.
Published: 15 August 2004
... in living cells using fusions of Smad2 and Smad4 with enhanced GFP. We first establish that the GFPSmad fusions behave like wild-type Smads in a variety of cellular assays. We go on to demonstrate directly, using photobleaching experiments, that Smad2 and Smad4 shuttle between the cytoplasm and nucleus...
Journal Articles
J Cell Sci (2004) 117 (8): 1443–1455.
Published: 15 March 2004
...Kazuhiko S. K. Uchida; Shigehiko Yumura We observed the dynamics of actin foci in live Dictyostelium cells expressing GFP-actin. Actin foci were dynamic structures, but they were fixed on the substratum during cell migration. Interference reflection microscopy revealed that the ventral cell...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2004) 117 (4): 641–652.
Published: 1 February 2004
...Rajani Srikakulam; Donald A. Winkelmann De novo folding and assembly of striated muscle myosin was analyzed by expressing a GFP-tagged embryonic myosin heavy chain (GFP-myosin) in post-mitotic C2C12 myocytes using replication defective adenoviruses. In the early stages of muscle differentiation...
Journal Articles
J Cell Sci (2003) 116 (20): 4227–4238.
Published: 15 October 2003
... encoding green fluorescent protein (GFP) fused to a striated muscle myosin heavy chain (GFP-myosin). Within 24 hours of transfection, the GFP-myosin is found co-assembled with the endogenous myosin in striated myofibrils. The wild-type GFP-myosin had no effect on the organization of the contractile...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2003) 116 (11): 2195–2201.
Published: 1 June 2003
... then placed on the microscope stage for microscopic imaging. However, we can estimate the coherence probabilities of multiple chromatids in endoreduplicated cells by comparing the average GFP spot numbers and the average DAPI fluorescence intensities in endoreduplicated pavement cells versus diploid...
Journal Articles
J Cell Sci (2003) 116 (8): 1611–1616.
Published: 15 April 2003
...Harald Jockusch; Sylvana Voigt We studied the migratory behaviour of adult muscle precursor cells in the mouse into and from skeletal muscle grafts using green fluorescent protein(GFP) and nuclear LacZ transgenes as complementary and double markers of the cell's origin. Owing to the small molecular...
Journal Articles
J Cell Sci (2002) 115 (14): 2893–2905.
Published: 15 July 2002
... Fluorescent Protein (GFP). When expressed in Dictyostelium cells, this fusion protein, VatM-GFP, was correctly targeted to contractile vacuole and endolysosomal membranes and was competent to direct assembly of the V-ATPase enzyme complex. Protease treatment of isolated endosomes indicated that the GFP moiety...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2002) 115 (12): 2549–2558.
Published: 15 June 2002
... in Saccharomyces cerevisiae is investigated. For this purpose, the localisation of Crh2p, a cell wall glycosylphosphatidylinositol (GPI)-anchored mannoprotein that we have recently described as involved in cell wall construction and localised to polarised growth sites, was followed using a Crh2p-GFP fusion protein...
Journal Articles
J Cell Sci (2002) 115 (11): 2399–2412.
Published: 1 June 2002
... and differences between transport organelles in differentiated neuroendocrine cells and neurons. * Author for correspondence (e-mail: bethe@lclark.edu ) 5 3 2002 © The Company of Biologists Limited 2002 2002 GFP PtdIns(4,5) P 2 Synaptophysin Frequenin Synaptotagmin Neurons...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2002) 115 (10): 2139–2149.
Published: 15 May 2002
...) PtdIns(4,5) P 2 (PH-GFP) ΔF/F Rate ×10 3 ΔPM/C (peak) Rate ×10 3 ΔPM/C (end) Delay (starts) Delay (peaks) Control ( n =9) 0.55±0.03 12.3±0.8 0.24±0.02 8.9±2.5 0.27±0.06 58±6 31±9 Wortmannin ( n =8) 0.54±0.04 13.4±1.3 0.09±0.03 † 2.1±1.4 * 0.02±0.03 † 80±10...
Journal Articles
J Cell Sci (2002) 115 (7): 1497–1510.
Published: 1 April 2002
... in the cell where they regulate localized activities. § Author for correspondence (e-mail: horwitz@virginia.edu ) 23 1 2002 © The Company of Biologists Limited 2002 2002 Cell adhesion GFP GIT1 p21 activated kinase (PAK) Rac Paxillin Cell migration is essential in many...
Includes: Multimedia, Supplementary data