... and GFP-tagged LaA completely rescued nuclear shape defects when expressed at similar levels in lamin A/C-deficient ( Lmna –/– ) MEFs, and all LaA constructs prevented increased nuclear envelope ruptures in these cells. N-terminal tags, however, altered the nuclear localization of LaA and impaired...

... zero; mean±s.e.m., n=7), compared to the recovery timecourse of the light response following PtdIns(4,5)P2 depletion by light stimuli calibrated to result in the same initial suppression in the same cells. Since the introduction of PLCδ1-PH–GFP ( Stauffer et al., 1998 ; Varnai and Balla, 1998...

... to CLSM because of their superior optical properties (less absorption and scattering of light) compared with whole tissues. Kubow and Horwitz used translucent collagen gels and low-light confocal microscopy to visualize formation of faint green fluorescent protein (GFP)-labelled paxillin adhesions (imaged...

... and overexpression phenotypes. Further analysis of two of these proteins, Rab18 and Rab43, strongly indicated roles in ER-Golgi trafficking. Rab43-T32N redistributed Golgi elements to ER exit sites without blocking trafficking of the secretory marker VSVG-GFP from ER to cell surface. Wild-type Rab43 redistributes...

... of ERK activation ( Horgan and Stork, 2003 ). Therefore, we determined ERK2 cytoplasmic-nuclear shuttling using confocal microscopy. GFP-ERK2 was transiently transfected in HeLa cells either with wild-type PKCγ-RFP or SCA14 mutant PKCγ-RFP and stimulated them with PMA. PKC activation induced a transient...

... rings and circumferential belts in epitheloid cells. J. Cell Sci. 97 , 297 -306. We thank T. Q. P. Uyeda and A. Nagasaki for providing constructs of GFP–α-tubulin, GFP-histone1 and mCherry-actin. We also thank T. Kitanishi-Yumura for helpful discussions. A part of this work was supported...

... cells, GFP-tagged versions of the Arabidopsis thaliana heterotrimeric G protein subunits were constructed. Mutational analysis showed that the Arabidopsis Gα subunit, GPα1, contains two lipidation motifs that were essential for plasma membrane localization. The Arabidopsis Gβ subunit, AGβ1, and the Gγ...

...Pankaj Dhonukshe; Norbert Vischer; Theodorus W. J. Gadella, Jr The spindle occupies a central position in cell division as it builds up the chromosome-separating machine. Here we analysed the dynamics of spindle formation in acentrosomal plant cells by visualizing microtubules labelled with GFP-EB1...

...Péter Várnai; Tzvetanka Bondeva; Péter Tamás; Balázs Tóth; László Buday; László Hunyady; Tamas Balla Several pleckstrin-homology (PH) domains with the ability to bind phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5) P 3 , PIP 3 ] were expressed as green fluorescent protein (GFP...

... posttranslational modification. Moreover, in transfected HeLa cells, chimeric GFP containing a nuclear localization signal and a C-terminal CaaX motif of N-Ras induces intranuclear membrane stacks that resemble those induced by lamins and ER-like cisternae that are induced in the cytoplasm upon increased synthesis...

... in living cells using fusions of Smad2 and Smad4 with enhanced GFP. We first establish that the GFPSmad fusions behave like wild-type Smads in a variety of cellular assays. We go on to demonstrate directly, using photobleaching experiments, that Smad2 and Smad4 shuttle between the cytoplasm and nucleus...

...Kazuhiko S. K. Uchida; Shigehiko Yumura We observed the dynamics of actin foci in live Dictyostelium cells expressing GFP-actin. Actin foci were dynamic structures, but they were fixed on the substratum during cell migration. Interference reflection microscopy revealed that the ventral cell...

...Rajani Srikakulam; Donald A. Winkelmann De novo folding and assembly of striated muscle myosin was analyzed by expressing a GFP-tagged embryonic myosin heavy chain (GFP-myosin) in post-mitotic C2C12 myocytes using replication defective adenoviruses. In the early stages of muscle differentiation...

... encoding green fluorescent protein (GFP) fused to a striated muscle myosin heavy chain (GFP-myosin). Within 24 hours of transfection, the GFP-myosin is found co-assembled with the endogenous myosin in striated myofibrils. The wild-type GFP-myosin had no effect on the organization of the contractile...

.... * Author for correspondence (e-mail: [email protected] ) 21 2 2003 © The Company of Biologists Limited 2003 2003 Endoreduplication Polyploidy Chromatin dynamics GFP Arabidopsis Fluorescence in situ hybridization analysis has shown that chromatin in eukaryote interphase...

...Harald Jockusch; Sylvana Voigt We studied the migratory behaviour of adult muscle precursor cells in the mouse into and from skeletal muscle grafts using green fluorescent protein(GFP) and nuclear LacZ transgenes as complementary and double markers of the cell's origin. Owing to the small molecular...

... Fluorescent Protein (GFP). When expressed in Dictyostelium cells, this fusion protein, VatM-GFP, was correctly targeted to contractile vacuole and endolysosomal membranes and was competent to direct assembly of the V-ATPase enzyme complex. Protease treatment of isolated endosomes indicated that the GFP moiety...

... in Saccharomyces cerevisiae is investigated. For this purpose, the localisation of Crh2p, a cell wall glycosylphosphatidylinositol (GPI)-anchored mannoprotein that we have recently described as involved in cell wall construction and localised to polarised growth sites, was followed using a Crh2p-GFP fusion protein...

... and differences between transport organelles in differentiated neuroendocrine cells and neurons. * Author for correspondence (e-mail: [email protected] ) 5 3 2002 © The Company of Biologists Limited 2002 2002 GFP PtdIns(4,5) P 2 Synaptophysin Frequenin Synaptotagmin Neurons...

... Phosphatidylinositol 4,5-bisphosphate Oocyte GFP References Abbott, A. L. and Ducibella, T. ( 2001 ). Calcium and the control of mammalian cortical granule exocytosis. Front. Biosci. 6 , D792 -D806. Abbott, A. L., Fissore, R. A. and Ducibella, T. ( 1999 ). Incompetence of preovulatory mouse...