... formation, Rab22A must be activated by a specific guanine-nucleotide-exchange factor (GEF); however, all of the GEFs that have been reported to exhibit Rab22A-GEF activity in vitro also activate Rab5A, an essential regulator of the clathrin-mediated endocytosis pathway, and no Rab22A-specific GEF has ever...

... endosomes initially contain Rab5, which is replaced by Rab7 on late endosomes prior to their fusion with lysosomes. Recruitment of Rab7 to endosomes requires the Mon1–Ccz1 guanine-nucleotide-exchange factor (GEF). Here, we show that full function of the Drosophila Mon1–Ccz1 complex requires a third...

... place across the Golgi. GEF Golgi RAB GTPase Exocytosis Multisubunit tethering complex Ministerio de Ciencia, Innovación y Universidades  BIO2015-65090R RTI2018-093344-B-I00 Comunidad de Madrid 10.13039/100012818 S2017/BMD-3691 InGEMICS-CM Transport protein...

...Brian S. Hercyk; Julie Rich-Robinson; Ahmad S. Mitoubsi; Marcus A. Harrell; Maitreyi E. Das ABSTRACT Cdc42, a conserved regulator of cell polarity, is activated by two GEFs, Gef1 and Scd1, in fission yeast. Why the cell needs two GEFs is unclear, given that they are partially redundant and activate...

..., distribution and reproduction in any medium provided that the original work is properly attributed. Summary: The development of a fluorescent reporter of GTPase activation based on tripartite split-GFP that enables the evaluation of GEF activity and the effect of modulators of GTPase activation...

... trafficking GEF GAP Ministry of Science and Technology 10.13039/100007225 MOST-103-2320-B-002-048-MY3 CMRPD3E0081-3 The ADP-ribosylation factors (Arfs) are members of a small GTPase family within the Ras superfamily that regulate membrane transport, organelle integrity, membrane lipid...

... and function. Ras signaling GEF GRF1 GRF2 Retina Cone photoreceptor Nuclear migration Intracellular traffic The RASGRF family of mammalian guanine nucleotide exchange factors (GEFs) has two highly homologous members, GRF1 and GRF2 (also known as RASGRF1 and RASGRF2...

... Highlighted Article: A combination of a Rab7 sensor and confocal FRET imaging techniques revealed that the Mon1–Ccz1 complex activates Rab7 only on late endosome and dissociates from lysosome in mammalian cells. Small GTPase FRET Endolysosomal pathway GEF Endocytosis and endolysosomal...

...-GTPases is spatio-temporally controlled at endothelial adherens junctions by guanine-nucleotide exchange factors (GEFs) is incompletely understood. Here, we identify a crucial role for the Rho-GEF Trio in stabilizing junctions based around vascular endothelial (VE)-cadherin (also known as CDH5). Trio...

...Nichol L. G. Miller; Christine Lawson; Elizabeth G. Kleinschmidt; Isabelle Tancioni; Sean Uryu; David D. Schlaepfer Summary Rgnef (also known as p190RhoGEF or ARHGEF28) is a Rho guanine-nucleotide-exchange factor (GEF) that binds focal adhesion kinase (FAK). FAK is recruited to adhesions...

... in Gas1p transport, cell wall integrity or vacuolar structure. These findings reveal that activation of Arl1p is regulated in part by Syt1p, and imply that Arl1p activation, by using more than one GEF, exerts distinct biological activities at the Golgi compartment. * These authors contributed...

... protein (CPEB) is required for polyadenylation-induced translation of mRNAs encoding cell cycle regulators. Xenopus Gef (XGef), a Rho-family guanine-exchange factor, influences the activating phosphorylation of CPEB. An exchange-deficient version of XGef does not, therefore implicating Rho-family GTPase...

... activity and epithelial-mesenchymal transition. J. Cell Biol. 149 , 775 -781. 16 6 2004 © The Company of Biologists Limited 2004 2004 Small GTPase GEF Tiam1 Actin cytoskeleton Cell adhesion Melanoma Migration Invasion The ability of cells to adopt a variety...

... Polarisation Rho GTPases GEF PAR proteins Polarity touches on essentially every aspect of cell and developmental biology. Cell polarity is of fundamental importance for proliferation, differentiation and morphogenesis; yet, despite these diverse contexts, its broad features, such as the ability...

..., PIKE-L, resides in multiple intracellular compartments. Nerve growth factor treatment leads to PIKE-S activation by triggering the nuclear translocation of phospholipase C (PLC)-γ1, which acts as a physiological guanine nucleotide exchange factor (GEF) for PIKE-S through its Src-homlogy 3 (SH3) domain...

... nucleotide exchange factor (GEF). P63RhoGEF encodes a 63 kDa protein containing a Db1 homology domain in tandem with a pleckstrin homology domain and is most closely related to the second Rho GEF domain of Trio. Northern blot and in situ analysis have shown that p63RhoGEF is mainly expressed in heart...

... for correspondence (e-mail: [email protected] ) 11 8 2001 © The Company of Biologists Limited 2001 2001 GEF GTPase Rho family PIX PAK The small GTPases of the Rho family play key roles in transducing extracellular stimuli into distinct responses including cell motility...

... protein contains pleckstrin (PH)and GEF homology domains that are conserved in several proteins implicated in signaling and growth control. Forced expression of wild-type Sbf1 in NIH 3T3 cells inhibited their proliferation and altered their morphology. These effects required intact PH, GEF...

... it is well established that the Sec7 domain functions as a guanine nucleotide exchange factor (GEF) for ADP-ribosylation factors (ARFs) and the PH domain anchors the proteins to membrane phosphoinositols, the function of the N-terminal domain is unknown. Here we show that the N terminus of B2-1 (residues 1...

...A. Blangy; E. Vignal; S. Schmidt; A. Debant; C. Gauthier-Rouvière; P. Fort ABSTRACT Rho GTPases regulate the morphology of cells stimulated by extracellular ligands. Their activation is controlled by guanine exchange factors (GEF) that catalyze their binding to GTP. The multidomain Trio protein...