... declare no competing or financial interests. © 2020. Published by The Company of Biologists Ltd 2020 Summary: The activity of the critical oncoprotein RAS can be negatively regulated by two groups of proteins, GAPs and RASSF proteins, as reviewed here. RAS RASSF GAP RASA1 NF1...

... inactive state. This cycle is regulated by guanine-nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Several efforts have been made in connecting the correct GEFs and GAPs to their specific Rab. Here, we aimed to identify GAPs for Rab7b, the small GTPase involved in transport from...

... dynamics. In an earlier phosphotyrosine proteomics study, we identified a novel Rho-GTPase activating protein, now known as ARHGAP42, as a likely biologically relevant Src substrate. ARHGAP42 is a member of a family of RhoGAPs distinguished by tandem BAR-PH domains lying N-terminal to the GAP domain. Like...

... trafficking GEF GAP Ministry of Science and Technology 10.13039/100007225 MOST-103-2320-B-002-048-MY3 CMRPD3E0081-3 The ADP-ribosylation factors (Arfs) are members of a small GTPase family within the Ras superfamily that regulate membrane transport, organelle integrity, membrane lipid...

...Carlene D. Moore; Erin E. Thacker; Jennifer Larimore; David Gaston; Alison Underwood; Brian Kearns; Sean I. Patterson; Trevor Jackson; Chris Chapleau; Lucas Pozzo-Miller; Anne Theibert Centaurin α1 is an Arf GTPase-activating protein (GAP) that is highly expressed in the nervous system...

...Kanamarlapudi Venkateswarlu; Kevin G. Brandom; Hongruo Yun GTPase activating proteins (GAPs) of the centaurin family regulate the actin cytoskeleton and vesicle trafficking through inactivation of the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. We report the functional...

... downregulation is p190, a ubiquitously expressed GTPase activating protein (GAP). We show that, in p190-deficient fibroblasts, the typical functional activities mediated by plexins (such as cell collapse and inhibition of integrin-based adhesion) are blocked or greatly impaired. Notably, the functional response...

.... To this end we isolated and characterized the A. gossypii homolog of the Saccharomyces cerevisiae BEM2 gene which is part of a network of rho-GTPases and their regulators required for bud emergence and bud growth in yeast. Here we show that the AgBem2 protein contains a GAP-(GTPase activating protein) domain...

...Joan M. Taylor; Marlene M. Macklem; J. Thomas Parsons ABSTRACT Graf, the GTPase regulator associated with focal adhesion kinase was previously shown to have GAP activity for Rho A and Cdc42 in vitro ( Hildebrand et al 1996 Mol. Cell Biol. 16: 3169-3178). In this study we sought to determine whether...

... which, like other calmodulin-containing myosins, exhibits maximal velocity of actin filaments (15 nm/second) in the absence of Ca 2+ . Native myosin-IXb exhibits GAP activity on rho. Class IX myosins may be an important link between rho and rho-dependent remodeling of the actin cytoskeleton...

... domain of protein kinase C and a putative GTPase-activating protein (GAP) domain of the rho/rac family of ras-like G-proteins. Northern blot analysis of 16 different human tissues revealed an ∼8 kb transcript that is most highly expressed in peripheral blood leukocytes, with somewhat lower levels...