...Christine Adhiambo; Thierry Blisnick; Géraldine Toutirais; Emmanuelle Delannoy; Philippe Bastin The atypical small G protein Rab-like 5 has been shown to traffic in sensory cilia of Caenorhabditis elegans , where it participates in signalling processes but not in cilia construction. In this report...

.... Furthermore, epistasis experiments pinpoint p38 MAPK as operating downstream of Dishevelleds. We also demonstrate that chemical inhibition of p38 MAPK restores Wnt3a-attenuated GSK3β kinase activity. We demonstrate the involvement of G-proteins and Dishevelleds in Wnt3a-induced p38 MAPK activation...

.... J., Hardy, R. W., Socolich, M. and Zuker, C. S. ( 1993 ). Arrestin function in inactivation of G protein-coupled receptor rhodopsin in vivo. Science 260 , 1910 -1916. Grigliatti, T. A., Hall, L., Rosenbluth, R. and Suzuki, D. T. ( 1973 ). Temperature-sensitive mutations in Drosophila...

...Jeong-Hyun Park; Bryan C. Jensen; Charles T. Kifer; Marilyn Parsons ABSTRACT We describe here a novel, evolutionarily conserved set of predicted G-proteins. The founding member of this family, TbNOG1, was identified in a two-hybrid screen as a protein that interacts with NOPP44/46, a nucleolar...

... of ligands from the cell surface to terminate their action ( Brown et al., 1983 ; Carpentier, 1989 , 1993 ). Clathrin Endocytosis G-protein Dynamin © 1997 by Company of Biologists 1997 13 10 1997 * Author for correspondence (e-mail: [email protected]...

... activation is required for optimal adhesion and invasion. PT blocks G-protein-mediated signals, suggesting that an extracellular factor is involved. This is not a serum component or an autocrine motility factor, since the PT effect was serum-independent, and PT did not inhibit motility. Therefore...

... and stimulates the G proteins ( Cassel & Pfeuffer, 1978 ). The A and B subunits of CT were each examined for the induction of DNA synthesis in temperature-arrested cultures of 3YltsG125 at 39-8°C. Fig. 4 shows that neither B subunit nor A subunit induced DNA synthesis even at a concentration as high as 25...

... of reverse-phase and Partisil SAX HPLC columns showed similar values for determinations by either method. The involvement of a G-protein in this chemotactic system was confirmed by the finding that accumulation of Ins(1,4,5) P 3 was elicited by the addition of GTP γS (5′-[gamma-thio]triphosphate) to saponin...

... suggest that the defect in mutant synag 7 is located between G-protein and adenylate cyclase, and not between receptor and G-protein. A factor in the supernatant is absent in synag 7 and appears to be essential for normal GTP binding, GTPase and activation of adenylate cyclase. This soluble heat-labile...

.... In contrast, the immunofluorescence corresponding to transductory G–proteins was confined in sperm to functionally defined regions in the head and tail, in a manner specific for each antibody. While RA/β γT, AS-1 and RAα39 all stained the entire acrosome, AS–6 and RAαT stained only the acrosomal tip...

... formation of inositol tris-and polyphosphates. Evidence was also presented for the involvement of a GTP-binding G-protein. Here we report evidence for the involvement of a ras gene product in the D. discoideum inositol phosphate pathway. Use was made of strains of Dictyostelium transformed with a wild-type...

... responses in non-adapted cells. We deduce that the site of adaptation to cyclic AMP stimulation is at the G-protein involved in this signalling pathway. Moreover, as GTP γ S was found to be unable to induce adaptation, we conclude that the mechanism of adaptation involves an action of the cyclic AMP...

...-cyclic AMP produced peaks of IP 3 at 5, 15 and 30 s in a manner comparable to that seen previously in non-permeabilized cells. The possible involvement of a GTP-binding protein (G-protein) in this IP 3 signal transduction pathway was investigated by studying the effects on such permeabilized amoebae...