... could be one explanation, but molecular and mechanistic evidence is lacking. In addition to its role in canonical G q pathway activation, here we demonstrate that Opn4 efficiently activates G i heterotrimers and signals through the G protein βγ. Compared with the low levels of G i pathway activation...

...Hisaki Hayashi; Abdullah Al Mamun; Miho Sakima; Motohiko Sato ABSTRACT Activator of G-protein signaling 8 (AGS8, also known as FNDC1) is a receptor-independent accessory protein for the Gβγ subunit, which was isolated from rat heart subjected to repetitive transient ischemia with the substantial...

...-associated variants of WDR36 affected its ability to modulate Gαq-mediated signalling by TPβ. We report that WDR36 acts as a new scaffold protein tethering a G-protein-coupled receptor, Gαq and PLCβ in a signalling complex. * Author for correspondence ( [email protected] ) 18 5 2011...

...-gif.fr © The Company of Biologists Limited 2009 2009 Golgi Secretory pathway Intra-Golgi trafficking Membrane curvature ALPS motif Tether Golgin Sphingolipid Glycerophospholipid ADP ribosylation factor G protein COPI Coatomer Glycosylation Because of its structural...

... the cation channels TRP and TRP-like (TRPL) are the targets of a prototypical G protein-coupled signaling pathway. We have recently found that the TRPL channel translocates between the rhabdomere and the cell body in a light-dependent manner. This translocation modifies the ion channel composition...

... are not known. Here we investigate the role of two important biochemical pathways - G-protein-dependent and phosphoinositide 3-kinase (PI3K)-dependent signaling - in directing PGC migration in zebrafish. We show that G proteins of the Gi family are essential for directional migration but not for PGC motility...

...Graeme Milligan ABSTRACT A range of approaches have recently provided evidence that G-protein-coupled receptors can exist as oligomeric complexes. Both homo-oligomers, comprising multiple copies of the same gene product, and hetero-oligomers containing more than one receptor have been detected...

... arrested, indicating regulation by GTP-binding proteins. The effect of GTPγS was mimicked by AlF 4 − and mastoparan, two drugs which are known to activate heterotrimeric G proteins. Pertussis toxin which prevents G i /G o protein activation completely abolished the effect of GTPγS and mastoparan...

...Oliver Nüße; Eva J. Neer ABSTRACT The heterotrimeric G protein G o is highly enriched in the growth cones of neuronal cells and makes up 10% of the membrane protein of growth cones from neonatal rat brain. We have used PC12 cells, a cell line that differentiates to a neuron-like phenotype...

... sera raised against recombinant proteins or consensus peptide sequences within proteins of interest. We found a number of heterotrimeric G proteins (Gα i3 , Gα o , Gα q11 , Gα s and Gβ subunits) and members of the small GTP-binding proteins expressed in eosinophils. Two subtypes of G-protein alpha...

... microtubule depolymerization. Ultrastructural observations indicate that Golgi disassembly occurs by budding of coated vesicles. This stage of the process is inhibited by GTPγS, AlF(3-5), transducin βγ subunits, and mastoparan, indicating the involvement of trimeric G proteins. At a later stage, vesicles lose...

... of spike-shaped oscillations and suggest that the role of PAF is to augment cAMP relay. PAF seems to act only a few times during this time period of two hours, since Ca 2+ entry adapted to a subsequent stimulus for about 30 minutes. PAF showed a reduced response in the G protein β − strain LW14...

... that the G protein activator aluminum fluoride, which stimulates transport to the surface at low fluoride concentrations as previously reported, inhibits transport at higher concentrations. In the nonpolarized fibroblast cell line CV-1, neither staurosporine nor aluminum fluoride stimulates transport...

...J. Bruno de Almeida; Eliezer J. Holtzman; Philip Peters; Louis Ercolani; Dennis A. Ausiello; Jennifer L. Stow ABSTRACT Heterotrimeric guanine nucleotide-regulatory (G) proteins are associated with a variety of intracellular membranes and specific plasma membrane domains. In polarized epithelial LLC...

...Stuart A. Arkett; S. Jeffrey Dixon; Stephen M. Sims ABSTRACT Osteoclasts are the cells responsible for the resorption of bone and other mineralized tissues. GTP-binding proteins (G proteins) play important roles in regulating the activity of many cell types; however, there is limited knowledge...

... is found in the cytoplasm and on Golgi membranes. The present study investigates the role of heterotrimeric G proteins in the regulation of p200 binding to Golgi membranes. An in vitro binding assay was used to measure the binding of cytosolic p200 to LLC-PK 1 cell microsomal membranes and to purified rat...

...Fanja Kesbeke; Peter J. M. Van Haastert; René J. W. De Wit; B. Ewa Snaar-Jagalska ABSTRACT Mutant Frigid A (fgdA) of Dictyostelium discoideum is defective in a functional Ga2 subunit of a G protein and is characterized by a complete blockade of the cyclic AMP-mediated sensory transduction steps...

... AMP binding resulted from changes in receptor affinity. The total number of receptors did not significantly change during oscillations. Our experiments also showed that both GTP and GDP abolished oscillations in receptor binding completely, presumably by acting via a G protein. The guanine nucleotides...

... depleted the cellular ATP levels by 40-60%, consistent with inhibition of transport at energy-requiring stages. The G protein that ac-cumulates in cells treated with CCCP is heterogeneous. Most of it is larger than the newly synthesized G protein, is acylated with palmitic acid, and is resistant...