...Kathrin Neuland; Neeti Sharma; Manfred Frick ABSTRACT Ca 2+ -dependent regulation of fusion pore dilation and closure is a key mechanism determining the output of cellular secretion. We have recently described ‘fusion-activated’ Ca 2+ entry (FACE) following exocytosis of lamellar bodies in alveolar...

... fusion, respectively. We further show that in up to 70% of the cases exocytosis does not result in significant release of the peptide cargo, likely because of a mechanism that leads to premature closure of the fusion pore. Release of nucleotides and protons occurred regardless of whether peptides were...

... Freeze-fracture Fusion pore Vacuolar H + -ATPases (V-ATPases) translocate protons across the membrane of various organelles (lysosomes, endosomes, trans-Golgi cisternae, secretory granules etc.), creating an electrochemical proton gradient that is acidic and positive within these intracellular...

... in the localized exocytosis. In the present report, scanning electron microscopy revealed collections of pits or craters, up to ∼1.5 μm in diameter. These pits are evidently fusion pores at sites of recent exocytosis, clustered in the immediate vicinity of the deepening furrow base and therefore near the furrow...