...Daisuke Kiyoshima; Keisuke Kawakami; Kimihide Hayakawa; Hitoshi Tatsumi; Masahiro Sokabe The effects of mechanical force applied to the integrin clusters at focal contacts were examined in cultured human umbilical vein endothelial cells. When a fibronectin-coated glass bead was attached...

...Keisuke Kawakami; Hitoshi Tatsumi; Masahiro Sokabe Human umbilical vein endothelial cells were stained with FITC-labeled anti-β 1 integrin antibody and plated on a glass cover slip to elucidate the mechanism of integrin clustering during focal contact formation. The process of integrin clustering...

... cytoskeleton in skeletal muscle. Eur. J. Cell Biol . 78 , 288 -295. Seifert, G. J., Lawson, D. and Wiche, G. ( 1992 ). Immunolocalization of the intermediate filament-associated protein plectin at focal contacts and actin stress fibers. Eur. J. Cell Biol . 59 , 138 -147. Smith, F. J. D...

... insights into the role of profilins in cellular motility. * Author for correspondence (e-mail: [email protected] ) 09 02 2000 21 03 2000 © 2000 by Company of Biologists 2000 Profilin Listeria monocytogenes Focal contact Actin dynamics Ena/VASP protein The actin...

... interaction with α 3β1 and α 6β4 integrins. SFL/LN5, and purified human laminin 5, induced the scattering and motility of MDCK cells and the formation of actin stress fibers and focal contacts in A549 cells. These events were dependent on activation of the small GTP-binding protein Rho. α v colocalized...

... of different plaque molecules, including vinculin, paxillin, tensin and phosphotyrosine-containing proteins. Ratio imaging revealed considerable molecular heterogeneity between and within adhesion sites. Most striking were the differences between focal contacts, which are vinculin- and paxillin-rich...

...Jorg Hagmann; Marianne Grob; Arkadiusz Welman; Gijsbert van Willigen; Max M. Burger ABSTRACT Platelets are anuclear, membrane-bounded fragments derived from megakaryocytes which, upon stimulation, assemble an actin skeleton including stress fibres and focal contacts. The focal contacts resemble...

... implicated in disassembly of established adhesions ( Hendey and Maxfield, 1993 ) to enable recycling of integrins to newly established adhesions at the migratory front ( Lawson and Maxfield, 1995 ). In fibroblasts migrating on defined substratum, in contrast, elements from many of the focal contacts are left...

... immunocytochemically distinct populations of β 1 integrins on fixed embryonic chick dermal fibroblasts. One population, recognized by the integrin activating mAb TASC, localizes to discrete regions of the cell, most likely focal contacts. These integrins co-localize with other proteins, such as vinculin and F-actin...

... constituents, and express only traces of the α6β4 integrin. By immunofluorescence and interference reflection microscopy HD1 was found together with vinculin at the end of actin filaments in focal contacts. In OVCAR-4 cells, derived from a human ovarian carcinoma which, like GD25 cells, only weakly express...

...Andre Tourkin; Michael Bonner; Ekaterina Mantrova; E. Carwile LeRoy; Stanley Hoffman ABSTRACT We have examined the organization of F-actin and focal contacts in eosinophils and the functional consequences correlated with their redistribution. In activated eosinophils adherent to laminin, F-actin...

..., antibodies specific for α3A stained most cell types in a diffuse pattern, consistent with previous reports. Surprisingly, however, chemical cross-linking of integrins to the extracellular matrix and extraction of the cytoskeleton prior to immuno-fluorescence revealed α3A in focal contacts of most cells...

... junctions. * Author for correspondence 25 07 1994 10 11 1994 © 1995 by Company of Biologists 1995 resonance energy transfer vinculin talin α-actinin actin focal contact cell adhesion Adherens-type junctions (AJ) are a family of cell-cell and cell-matrix adhesions...

...). Occasionally, parallel stripes were seen at the edges of adhesion plaques ( Fig. 1A , arrowhead), reminiscent of the ‘needle eye’ patterns described by Damsky et al. (1985) . 23 07 1993 20 12 1993 © 1994 by Company of Biologists 1994 adhesion plaque focal contact fibronectin...

... and staining for F-actin appeared in patches. Vinculin, one of the components of focal contacts, was localized at the periphery of control cells. CL-A treatment moved the focal contacts towards the inside of the cell along the stress fibers, and this was followed by the rounding up of the cell. In addition...

...Harald Grenz; Salvatore Carbonetto; Simon L. Goodman ABSTRACT The movement of integrins into focal adhesive structures accompanies cell attachment to extracellular matrix. The kinetics of incorporation of integrins into focal contacts was studied during attachment to matrix of mesangial cells...

... of such contacts depend on the thickness of the lamellae. Plaque-like focal contacts, distinguished on the basis of shape and low irradiance (darkness), are intimate adhesions to the substratum. These images can be interpreted if it is assumed that microfllaments associated with the lower membrane increase...