... to inhibiting actin glutathionylation, LCN2 stimulates invasion by promoting the formation of focal adhesions, which is independent of the ability of LCN2 to bind iron. LCN2 promotes focal adhesion formation by promoting the activation of c-Src by stimulating the expression of the transcription factor ETS1...

... and focal adhesion signalling. Here, we show that constitutive activation of c-Src in endothelial cells results in rapid vascular expansion, operating independently of growth factor stimulation or fluid shear stress forces. This is driven by an increase in focal adhesion signalling and size...

... in HSC activation and liver fibrosis. Although these findings are novel, they are consistent with a previous study showing that focal adhesion kinase (FAK, also known as PTK2, another important FA protein) promotes HSC activation in vitro and liver fibrosis in vivo ( Zhao et al., 2017 ). Given...

... the spatial organization within focal adhesions by analyzing several adhesion proteins with structured illumination microscopy (SIM). Paxillin (Pax) serves as a scaffold protein and signaling hub in focal adhesions, and focal adhesion kinase (FAK, also known as PTK2) regulates the dynamics of adhesions. We...

..., integrin α6β4 distinctly distributed peripherally as well as centrally, proximal to the nucleus. Upon fibronectin addition, integrin α6β4 was centrally recruited to large focal adhesions (FAs) and enhanced Fak (also known as PTK2) phosphorylation. Integrin β4 plectin-binding mutants or genetic deletion...

..., cell spreading on galectin-8-coated substrates resulted in increased projected cell area, more-pronounced extension of filopodia and, yet, the inability to form focal adhesions and stress fibers. These differences can be partially reversed by experimental manipulations of small G-proteins of the Rho...

... that drive early stages during the development of focal adhesions and invadopodia, and review distinct processes that lead to the maturation of the two integrin-associated adhesions. We hypothesize that adhesions to the ECM in both structures are initiated by shear forces that are generated by nearby...

... , 1655 - 1699 . 10.1152/physrev.00036.2018 Ballestrem , C. , Hinz , B. , Imhof , B. A. and Wehrle-Haller , B. ( 2001 ). Marching at the front and dragging behind: differential alphaVbeta3-integrin turnover regulates focal adhesion behavior . J. Cell Biol.   155 , 1319 - 1332...

.... Cell Sci.   124 , 820 - 830 . 10.1242/jcs.078832 Calalb , M. B. , Polte , T. R. and Hanks , S. K. ( 1995 ). Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for Src family kinases . Mol. Cell. Biol.   15...

... ligand tether length affects the size and length of focal adhesions and influences cell spreading and attachment . Sci. Rep.   6 , 34334 . 10.1038/srep34334 Augst , A. D. , Kong , H. J. and Mooney , D. J. ( 2006 ). Alginate hydrogels as biomaterials . Macromol. Biosci.   6...

... and effects on cell proliferation. On fibronectin, GFP-tagged β1A integrin showed dynamic exchange in peripheral focal adhesions, and long, central fibrillar adhesions. In contrast, GFP–β1D integrins exchanged slowly, forming immobile and short central adhesions. While adhesion recruitment of GFP–β1A integrin...

... GTPases (Rap1a and Rap1b, referred to collectively as Rap1). Under these conditions Rap1 activity stimulates the formation of focal adhesion structures that align with the tensional axis as single tumor cells move into the matrix. These effects are mediated by the ability of Rap1 to induce the polarized...

... and talin2, respectively. Talin1 regulates focal adhesion dynamics, cell migration and invasion, whereas the biological function of talin2 is not clear and, indeed, talin2 has been presumed to function redundantly with talin1. Here, we show that talin2 has a much stronger binding to β-integrin tails than...

...Adil R. Sarhan; Trushar R. Patel; Alana R. Cowell; Michael G. Tomlinson; Carina Hellberg; John K. Heath; Debbie L. Cunningham; Neil A. Hotchin ABSTRACT Focal adhesions are complex multi-molecular structures that link the actin cytoskeleton to the extracellular matrix through integrin adhesion...

...Ariel Livne; Benjamin Geiger ABSTRACT Ventral stress fibers and focal adhesions are physically coupled structures that play key roles in cellular mechanics and force sensing. The tight functional interdependence between the two is manifested not only by their apparent proximity but also by the fact...

...I-Husan Huang; Cheng-Te Hsiao; Jui-Chung Wu; Rong-Fong Shen; Ching-Yi Liu; Yang-Kao Wang; Yu-Chen Chen; Chi-Ming Huang; Juan C. del álamo; Zee-Fen Chang; Ming-Jer Tang; Kay-Hooi Khoo; Jean-Cheng Kuo ABSTRACT Focal adhesions (FAs) undergo maturation that culminates in size and composition changes...

...Pradeep D. Uchil; Tobias Pawliczek; Tracy D. Reynolds; Siyuan Ding; Angelika Hinz; James B. Munro; Fang Huang; Robert W. Floyd; Haitao Yang; William L. Hamilton; Joerg Bewersdorf; Yong Xiong; David A. Calderwood; Walther Mothes ABSTRACT Focal adhesions are macromolecular complexes that connect...

...Ching-Wei Chang; Sanjay Kumar Summary Actomyosin stress fibers (SFs) enable cells to exert traction on planar extracellular matrices (ECMs) by tensing focal adhesions (FAs) at the cell–ECM interface. Although it is widely appreciated that the spatial and temporal distribution of these tensile...

... role of srGAP3 on actin dynamics, specifically on lamellipodia formation. We show that the F-BAR domain localizes srGAP3 to the leading edge of cellular protrusions whereas the SH3 domain is important for focal adhesion targeting. We report on a novel srGAP3 interaction partner, lamellipodin, which...

... ) ‡ These authors contributed equally to this work 10 1 2011 © 2011. 2011 Cell spreading Ezrin Focal adhesions Rap1 The response of cells following contact with an extracellular matrix, either for spreading or for migration, requires the tightly controlled regulation of cell adhesion...