... cells, which contain an amplification of CASKIN2 , Caskin2 localizes in plasma membrane-associated plaques and around focal adhesions in cortical microtubule stabilization complexes. Taken together, our results identify Caskin2 as a novel talin-binding protein that might not only connect integrin...

... vascular stability. The spatiotemporal activation of the transcriptional co-factors YAP (herein referring to YAP1) and TAZ (also known WWTR1), collectively denoted YAP/TAZ, is crucial to allow for efficient collective endothelial migration in angiogenesis. The focal adhesion protein deleted-in-liver-cancer...

... localization in HeLa and U2OS cells further revealed S100A11 enrichment at focal adhesions (FAs). Strikingly, S100A11 levels at FAs increased sharply, yet transiently, just before FA disassembly. Elevating intracellular Ca 2+ levels with ionomycin stimulated both S100A11 recruitment and subsequent FA...

..., including the extracellular matrix (ECM). Cells adhere to ECM through focal adhesions (FAs) and sense the surrounding microenvironments. Although FA proteins have been actively investigated, little is known about the lipids in the plasma membrane at FAs. In this study, we examine the lipid composition...

...Alba Zuidema; Wei Wang; Maaike Kreft; Onno B. Bleijerveld; Liesbeth Hoekman; Jonas Aretz; Ralph T. Böttcher; Reinhard Fässler; Arnoud Sonnenberg ABSTRACT The vitronectin receptor integrin αVβ5 can reside in two distinct adhesion structures – focal adhesions (FAs) and flat clathrin lattices (FCLs...

... attributed. Highlighted Article: The autophagy-linked FYVE protein (ALFY) localizes to cell protrusions and early endosomes, and regulates directional cell migration and attachment by modification of integrin glycosylation. ALFY WDFY3 Endosome Migration Focal adhesion Integrin...

... contain the non-neuronal proteins LL5β (also known as PHLDB2) and KANK1, which, in migrating cells, organize exocytotic machinery in the vicinity of integrin-based adhesions. Depletion of LL5β or focal adhesion disassembly triggered by myosin II inhibition perturbed the clustering of secretory complexes...

...Yi-Chun Liao; Su Hao Lo ABSTRACT Tensins are a family of focal adhesion proteins consisting of four members in mammals (TNS1, TNS2, TNS3 and TNS4). Their multiple domains and activities contribute to the molecular linkage between the extracellular matrix and cytoskeletal networks, as well...

..., mitogen-activated protein 4 kinase 4 (MAP4K4) was identified as important novel mediator of endothelial permeability, which signaled via ezrin, radixin and moesin proteins to increase turnover of integrin-based focal adhesions. The combined inhibition of MAP4K4 and Abl-related gene (Arg, also known...

... of GFP–FAK after photobleaching of focal adhesions from cells pre-treated with vehicle (Ctrl, blue square, n =18), T101 (purple circles, n =15) or MDC (red circles, n =15). The normalized mean intensity is shown along with the exponential fit along with (B) the corresponding quantification...

... their participation in adhesion turnover and cellular signalling. In this Review, we focus on the interactions between microtubules and integrin-mediated adhesions, in particular, focal adhesions and podosomes. Starting with the association of microtubules with these adhesive structures, we describe the classical...

... density of inward-rectifier K + channels (K ir 2.1; encoded by KCNJ2 ) and profoundly alter the action potential shape of excitable cells. By using micropatterning to manipulate the localization and size of focal adhesions (FAs) in single HEK293 cells engineered to stably express K ir 2.1 channels...

... regulates adhesion complex composition and phosphotyrosine signaling. Talin rod subdomains and their mechanical activation are required for cell polarization and migration. BioID Focal adhesion Mechanobiology Phosphoproteomics Protein engineering Talin Academy of Finland 10.13039...

... gene), both of which localize to focal adhesions, cooperatively function as mechanosensors of ECM stiffness. On a rigid ECM, vinexin α interacts with vinculin and induces a conformational change in vinculin to give an ‘open’ form, which promotes nuclear localization of Yes-associated protein (YAP, also...

... of multiple actin-based structures, likely by regulation of integrin signaling. Changes in focal adhesion organization and basal actin stress fibers in MRP-knockout (KO) cells were reminiscent of those seen in FAK-KO cells. In addition, we found alterations in cell–cell interactions in MRP-KO cells associated...

... of the accumulated integrins. Mechanistically, ADAM9 co-immunoprecipitates with β1 integrin, and both internalization and subsequent degradation of β1 integrin are significantly decreased in ADAM9-silenced cells, with no effect on β1 integrin recycling. Accordingly, the formation of focal adhesions and actin stress...

...) constitute an adaptor protein family called the vinexin (SORBS) family that is targeted to focal adhesions (FAs). Although numerous studies have focused on each of the SORBS proteins and partially elucidated their involvement in mechanotransduction, a comparative analysis of their function has not been well...

...-licence-1-1/ Summary: ARHGAP42 is activated by Src-mediated Tyr-376 phosphorylation to inhibit RhoA, and promote focal adhesion dynamics and cell motility. Src Motility RhoA GAP GRAF Focal adhesion Tyrosine phosphorylation National Institutes of Health 10.13039/100000002...

... cells significantly reduced invadopodia formation and matrix degradation, and simultaneously increased overall cell adhesion to the ECM. Live-imaging analysis showed that knockdown of ADAM12 significantly inhibited focal adhesion turnover. Mechanistically, both the disintegrin and metalloproteinase...

...Ben Stutchbury; Paul Atherton; Ricky Tsang; De-Yao Wang; Christoph Ballestrem ABSTRACT Focal adhesions (FAs) are macromolecular complexes that regulate cell adhesion and mechanotransduction. By performing fluorescence recovery after photobleaching (FRAP) and fluorescence loss after photoactivation...