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Keywords: Fluorescent protein
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Journal Articles

Toxicity of the model protein 3×GFP arises from degradation overload, not from aggregate formation

Open Access
In collection:
Proteostasis
Shotaro Namba, Hisao Moriya
Journal: Journal of Cell Science
J Cell Sci (2024) 137 (11): jcs261977.
https://doi.org/10.1242/jcs.261977
Published: 12 June 2024
... green fluorescent protein-linked protein (3×GFP) induces giant aggregates and is detrimental to growth. Here, we investigated the mechanism of growth inhibition by 3×GFP-op using non-aggregative 3×MOX-op as a control in Saccharomyces cerevisiae . The 3×GFP aggregates were induced by misfolding, and 3...
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Includes: Supplementary data
Journal Articles

Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity

Open Access
In collection:
Imaging , Review Commons Transfers
Eike K. Mahlandt, Gabriel Kreider-Letterman, Anna O. Chertkova, Rafael Garcia-Mata, Joachim Goedhart
Journal: Journal of Cell Science
Series: REVIEW COMMONS TRANSFER
J Cell Sci (2023) 136 (10): jcs260802.
https://doi.org/10.1242/jcs.260802
Published: 25 May 2023
.... These optimized sensors enable the wider application of Rho GTPase relocation sensors, which was showcased by the detection of local endogenous Cdc42 activity at assembling invadopodia. Moreover, we tested several fluorescent proteins and HaloTag for their influence on the recruitment efficiency of the Rho...
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Includes: Supplementary data
Journal Articles

Plasmodesmata at a glance

Free
Ross E. Sager, Jung-Youn Lee
Journal: Journal of Cell Science
J Cell Sci (2018) 131 (11): jcs209346.
https://doi.org/10.1242/jcs.209346
Published: 7 June 2018
... of Biologists Ltd 2018 Summary: This Cell Science at a Glance article summarizes how plasmodesmata are dynamic and multifaceted nanochannels that bridge neighboring plant cells. Cell communication Cell signaling Fluorescent protein Membrane nanotube Plasmodesmata Protein trafficking Cell...
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Includes: Supplementary data