...Aaron R. Tipton; Gary J. Gorbsky ABSTRACT The microtubules of the mitotic spindle mediate chromosome alignment to the metaphase plate, then sister chromatid segregation to the spindle poles in anaphase. Previous analyses of spindle microtubule kinetics utilizing fluorescence dissipation after...

...Christopher Estell; Emmanouela Stamatidou; Sarah El-Messeiry; Andrew Hamilton ABSTRACT Although mitochondrial translation produces only 13 proteins, we show here how this process can be visualised and detected in situ by fluorescence microscopy with a simple, rapid and inexpensive procedure using...

...Eugenio Gallo; Jonathan W. Jarvik ABSTRACT A novel bi-partite fluorescence platform exploits the high affinity and selectivity of antibody scaffolds to capture and activate small-molecule fluorogens. In this report, we investigated the property of multi-selectivity activation by a single antibody...

.... ( 2007 ). Multicolor super-resolution imaging with photo-switchable fluorescent probes . Science   317 , 1749 - 1753 . 10.1126/science.1146598 Berika , M. and Garrod , D. ( 2014 ). Desmosomal adhesion in vivo . Cell Commun. Adhes.   21 , 65 - 75 . 10.3109/15419061.2013.876018...

...Liang Gao; Alejandro Garcia-Uribe; Yan Liu; Chiye Li; Lihong V. Wang ABSTRACT We present a generic sub-diffraction-limited imaging method – photobleaching imprinting microscopy (PIM) – for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a twofold improvement...

... to contribute to future solutions. * Author for correspondence ( timm.schroeder@bsse.ethz.ch ) © 2013. Published by The Company of Biologists Ltd 2013 Biosensors Fluorescence Hardware Single-cell imaging Software Time-lapse microscopy Organisms are composed of vast numbers...

.... It is especially true in cells where the spatiotemporal distribution of Tau–MT interactions is unknown. Using Förster resonance energy transfer (FRET), we showed that the Tau–MT interaction was distributed along MTs in periodic hotspots of high and low FRET intensities. Fluorescence recovery after photobleaching...

...Alexa L. Mattheyses; Sanford M. Simon; Joshua Z. Rappoport Total internal reflection fluorescence (TIRF) microscopy can be used in a wide range of cell biological applications, and is particularly well suited to analysis of the localization and dynamics of molecules and events near the plasma...

... for correspondence ( maddy.parsons@kcl.ac.uk ) © 2010. 2010 Cell adhesion Fluorescence Focal adhesion Live imaging Microscopy Migration Several different types of adhesions are formed by cells following engagement with the extracellular matrix (ECM). These structures are classified on the basis...

... for correspondence (e-mail: tamkunmm@lamar.colostate.edu ) 9 5 2007 © The Company of Biologists Limited 2007 2007 Cytoskeletal fence Fluorescence Live cell imaging Localization Potassium channel Quantum dot tracking Fig. 9. Concentration-dependent effects of swinholide A on GFP...

... by a donkey anti-goat RRX secondary antibody. The collected images show 100% colocalization with the receptors in addition to some background fluorescence. To measure the distribution of the BMP receptors on the cell surface, we used image correlation spectroscopy (ICS) and ICCS. A431 cells were cultured...

... COS7 cells with plasmids encoding either HA- or myc-tagged BRIa alone or in combination with HA- or myc-tagged BRII. Cells were fluorescently labelled for the HA- or myc-BRIa and HA- or myc-BRII receptor expression and confocal images were collected ( Fig. 1 ). To determine the specificity...

...Jeffrey M. Levsky; Robert H. Singer Fluorescence in situ hybridization (FISH), the assay of choice for localization of specific nucleic acids sequences in native context, is a 20-year-old technology that has developed continuously. Over its maturation,various methodologies and modifications have...

...Pamela K. Hanson; Althea M. Grant; J. Wylie Nichols At low temperature, the short-chain fluorescent-labeled phospholipids,1-myristoyl-2-[6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminocaproyl]-phosphatidylcholine (M-C 6 -NBD-PC) and its phosphatidylethanolamine analog, M-C 6 -NBD-PE, are internalized...

...-tails promise to give new insights into the stability and lifetime of membrane domains. © The Company of Biologists Limited 1993 1993 plasma membrane lateral diffusion fluorescence FPR domains Journal o f Cell Science, Supplement 17, 165-169 (1993) Printed in Great Britain © The Company...

...Richard G. Sleight; Marina N. Abanto ABSTRACT The transport and metabolism of a fluorescent phosphatidylcholine analog, l-palmitoyl-2-(N-4-nitrobenzo-2-oxa-l,3-diazole)-aminocaproyl-phos-phatidylcholine ((palmitoyl, C 6 -NBD)-PC), in BHK, CH0-K1, CHO-15B, MDCK, VA-2, Vero, V79 and WI-38 cells has...

... as stemming from a closer cell–substratum apposition beneath the lamellae than the cell body. This ambiguity can be avoided if the technique of total internal reflection aqueous fluorescence (TIRAF) is used in conjunction with a high refractive index glass (n = 1·83) as substratum. Contributions to the image...