..., inexpensive and non-destructive identification of lipid content, and highlights the differences in lipid biology between cell types by imaging and flow cytometry. This article has an associated First Person interview with the first author of the paper . * Present address: Institute of Bioorganic...

.... Activation-induced gains in fluorescence were also observed in naïve and CD4 + T cells from the Lifeact-EGFP reporter, and were readily detected by both flow cytometry and live cell microscopy. This unique, translationally controlled reporter of effector T cell activation simultaneously enables tracking...

...Thomas D. Williams; Robert R. Kay ABSTRACT Macropinocytosis is a conserved endocytic process used by Dictyostelium amoebae for feeding on liquid medium. To further Dictyostelium as a model for macropinocytosis, we developed a high-throughput flow cytometry assay to measure macropinocytosis...

... during S phase progression. High-resolution data reveal the culmination of replication in the mid S phase in the nucleolus. A. thaliana rDNA Replication Nucleolus Flow cytometry Structured illumination microscopy Czech Science Foundation 10.13039/501100001824 16-04166Y...

... of autophagy using Acridine Orange, considering the red-to-green fluorescence intensity ratio (R/GFIR) to quantify flow cytometry and fluorescence microscopy data of Acridine-Orange-stained cells. This method measured with accuracy the increase in autophagy induced by starvation or rapamycin, and the reduction...

... sperm and that ENaCs are blocked during capacitation. In the present work, we used flow cytometry to analyze changes in intracellular Na + concentration ([Na + ] i ) during capacitation in individual cells. Our results indicate that capacitated sperm have lower Na + concentrations. Using sperm...

...Zakaria Hmama; Khalid Sendide; Amina Talal; Rosa Garcia; Karen Dobos; Neil E. Reiner Macrophage cell membranes were labeled with PKH26 and subsequently incubated with latex beads to generate phagosomes surrounded by a red-fluorescent membrane suitable for flow cytometry. Following cell disruption...

...-u.ac.jp ) 27 10 2000 © 2001 by Company of Biologists 2001 Mitochondrial biogenesis Microtubules Microtubule-active drugs Fluorescent dyes Flow cytometry Taxol Biogenesis of mitochondria proceeds in two main steps as proposed by Attardi and Schatz (Attardi and Schatz, 1998...

... for correspondence (e-mail: [email protected] ) * These authors contributed equally to this work 04 10 2000 10 08 2000 © 2000 by Company of Biologists 2000 Cell ageing Biological marker Beta-galactosidase Vascular endothelium Flow cytometry Lysosome Acid β-D...

... by Company of Biologists 1999 CEM cells bcl-2 Death Apoptosis Acidification Flow cytometry Apoptosis is a regulated cell death of importance in cell homeostasis, neoplasia and neurodegeneration ( Arends and Wyllie, 1991 ). One of the central mediators of apoptosis is the caspase family...

...), Carleton University, 1125 Colonel By Drive, Ottawa ON K1S 5B6, Canada (e-mail: [email protected] ) 08 06 1998 30 07 1998 © 1998 by Company of Biologists 1998 T lymphocytes B lymphocytes NuMA Apoptosis Hyperthermia DNA fragmentation Immunocytochemistry Flow cytometry...

.... * Author for correspondence 05 11 1993 26 04 1994 © 1994 by Company of Biologists 1994 human keratinocyte cell culture flow cytometry cell cycle growth arrest transforming growth factor P1 Epidermis is a dynamic tissue in which highly coordinated mechanisms exist...

...Miriam J. Smyth; Rodney L. Sparks; Walker Wharton * Author for correspondence © 1993 by Company of Biologists 1993 differentiation proliferation adipogenesis growth arrest adipocyte-specific gene expression flow cytometry The relationship between growth arrest...

... 28 01 1992 © 1992 by Company of Biologists 1992 PCNA DNA/nuclear proteins flow cytometry There is increasing interest in studies on the regulatory mechanisms of the cell cycle. The ability of cells to begin a new cell cycle is a prerequisite for cell reproduction ( Pardee...

... by both Southern blot analysis and flow cytometry of cells stained with a variety of DNA-binding fluorochromes, which we show are able to detect nuclear and mitochondrial DNA with different efficiencies. In the conditional cell division cycle mutant cdclO , which is unable to initiate nuclear DNA...

... as the doubling time in cell length. Flow cytometry showed that the cells had increased their DNA content from 4C (the diploid value) to about 16C after 7 h. The cytological appearance at this time was mixed, with uninucleate, binucleate and dead cells, but fluorescence measurements on single cells indicated...

...B. Novak; J. M. Mitchison ABSTRACT We show that the first of the two transition points of cdc2.33, a mutant of Schizosaccharomyces pombe , exists in exponential phase cells. Using flow cytometry and a double-block experiment, we have measured the position of this transition point both in the single...

... in conjunction with flow cytometry analysis to study the differential growth of the luminal and myoepithelial cells in organoid culture. These studies will form the basis of a separate report (Dundas, O’Hare, Ormerod and Gusterson, work in progress). This approach should permit not only direct studies of growth...

... of the chromosome scaffold. Quantification of the Ki-67 fluorescence signal using flow cytometry revealed the highest staining intensities in mitotic cells. Furthermore, it was shown that nutritionally deprived cells became negative for Ki-67. * Author for correspondence 03 10 1988 16 12 1988...

... Acanthamoeba castellanii cell cycle encystation anti-BrdUrd labelling flow cytometry During the growth phase of Acanthamoeba castellanii little spontaneous encystment of trophozoites takes place. However, encystation can be induced by’ starvation, desiccation, high salt concentration and several...