... Riemer; Tuguldur Tumurbaatar; Kristina Djinovic-Carugo; Gang Dong; Christian J. Janzen; Brooke Morriswood ABSTRACT All endocytosis and exocytosis in the African trypanosome Trypanosoma brucei occurs at a single subdomain of the plasma membrane. This subdomain, the flagellar pocket, is a small vase-shaped...

... have identified and characterized one of the enzymes that is responsible for generating PI(4,5)P 2 . Surprisingly, this phosphoinositide was found to be highly concentrated in the flagellar pocket, the only site of endocytosis and exocytosis in this organism. The enzyme (designated TbPIPKA, annotated...

... in the process of cell division. These aberrations include delay in basal body and kinetoplast separation, cleavage furrow progression and flagellar pocket division. In addition to these changes, the intracellular trafficking and actin dynamics are also adversely affected. All these abnormalities are, however...

...Sylvain Lacomble; Sue Vaughan; Catarina Gadelha; Mary K. Morphew; Michael K. Shaw; J. Richard McIntosh; Keith Gull This study uses electron tomography linked to a variety of other EM methods to provide an integrated view of the flagellar pocket and basal body area of the African trypanosome...

...Sabrina Absalon; Thierry Blisnick; Mélanie Bonhivers; Linda Kohl; Nadège Cayet; Géraldine Toutirais; Johanna Buisson; Derrick Robinson; Philippe Bastin In trypanosomes, the flagellum is rooted in the flagellar pocket, a surface micro-domain that is the sole site for endocytosis and exocytosis...

...Kevin J. Schwartz; Ronald F. Peck; Ngii N. Tazeh; James D. Bangs Progression of GPI-anchored proteins in bloodstream African trypanosomes correlates with GPI-valence: homodimeric VSG (2 GPI) is a surface protein; heterodimeric transferrin receptor (1 GPI) localizes in the flagellar pocket...

... the flagellar pocket, which actively communicates by vesicular membrane flow with cisternal/tubulovesicular endosomes. The division of the cell surface into three morphologically distinct sub-domains and the rapid plasma membrane turnover establishes T. brucei as an interesting model for investigations...

... The Company of Biologists Limited 2002 2002 Trypanosome Lysosome Flagellar pocket Endocytosis LAMP African trypanosomes are parasitic protozoa that cause human (sleeping sickness) and veterinary (Nagana) disease throughout the sub-Saharan continent. Phylogenetically, trypanosomes represent...

... or markers. Using concanavalin A internalisation and temperature block procedures, TbRAB11 was observed on endomembranes anterior to the flagellar pocket that are juxtaposed to the collecting tubules. TbRAB11 colocalised with the trypanosomal transferrin receptor and internalised antivariant surface...

... of CPB in vivo . When CPB C25G was expressed in a L. mexicana mutant lacking both CPA and CPB genes, the majority of the pro-enzyme remained unprocessed and accumulated in the flagellar pocket. These data reveal that CPA can directly or indirectly process CPB C25G and suggest that cysteine proteases...

...). Comparison with bisbenzimide-stained parasites (Fig. 7c) or by overlay of the images (Fig. 7d) this area is localized between flagellar pocket and nucleus. Immunoblot of parasite extracts with anti-tGLP-1 CT antibodies revealed a single band that corresponded to the species identified by Ali I-278 (data...

...Kent L. Hill; Nathan R. Hutchings; David G. Russell; John E. Donelson ABSTRACT The flagellar pocket of African trypanosomes is a critical sorting station for protein and membrane trafficking, and is considered to be an Achilles’ heel of this deadly pathogen. Although several proteins, including...

... of bloodstream forms of Trypanosoma brucei rhodesiense . Both markers were rapidly endocytosed from the flagellar pocket. Within 20 minutes at 37°C the markers reached a large, vesicular, perinuclear compartment that stained heavily with the CB1 monoclonal antibody. Neither marker left the flagellar pocket...

...Marla Jo Brickman; Andrew E. Balber ABSTRACT gp57/42 is a membrane glycoprotein localized in the trans - Golgi, flagellar pocket region of the cell surface, endosomes and lysosomes of bloodstream forms of Trypanosoma brucei rhodesiense . Pulse-chase immunoprecipitation experiments revealed...