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Keywords: Fission yeast
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Journal Articles
J Cell Sci jcs.258865.
Published: 09 September 2021
..., which recruits mTORC1 substrates, such as S6K1 and 4EBP1, by interacting with their TOR signaling (TOS) motif. Despite the evolutionary conservation of TORC1, no TOS motif has been described in lower eukaryotes. Here, we show that the fission yeast S6 kinase Psk1 contains a TOS motif that interacts...
Journal Articles
J Cell Sci jcs.258774.
Published: 09 September 2021
...Zhong-Qiu Yu; Xiao-Man Liu; Dan Zhao; Dan-Dan Xu; Li-Lin Du Protein-protein interactions are vital for executing nearly all cellular processes. To facilitate the detection of protein-protein interactions in living cells of the fission yeast Schizosaccharomyces pombe , here we present an efficient...
Journal Articles
J Cell Sci (2021) 134 (16): jcs258645.
Published: 17 August 2021
... ). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast) . Mol. Cell. Proteomics 13 , 1925 - 1936 . 10.1074/mcp.M113.035824 Chen , J.-S. , Beckley , J. R. , McDonald , N. A. , Ren , L. , Mangione , M. , Jang , S. J...
Includes: Supplementary data
Journal Articles
J Cell Sci (2021) 134 (6): jcs253021.
Published: 26 March 2021
...Sanju Ashraf; Ye Dee Tay; David A. Kelly; Kenneth E. Sawin ABSTRACT Movement of the cell nucleus typically involves the cytoskeleton and either polymerization-based pushing forces or motor-based pulling forces. In the fission yeast Schizosaccharomyces pombe , nuclear movement and positioning...
Includes: Supplementary data
Journal Articles
J Cell Sci (2021) 134 (3): jcs251769.
Published: 11 February 2021
...Elizabeth Scotchman; Kazunori Kume; Francisco J. Navarro; Paul Nurse ABSTRACT Fission yeast cells divide at a similar cell length with little variation about the mean. This is thought to be the result of a control mechanism that senses size and corrects for any deviations by advancing or delaying...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (23): jcs247973.
Published: 03 December 2020
... decades of study, our understanding of how each of these enzymes is coordinated in space and time to control PIP levels and their downstream effectors remains incomplete. Phosphoinositides Lipid kinase Opy1 Fission yeast Summary: The fission yeast dual PH domain-containing protein Opy1...
Includes: Supplementary data
Journal Articles
In collection:
Polarity
J Cell Sci (2020) 133 (18): jcs244392.
Published: 17 September 2020
...K. Adam Bohnert; Anthony M. Rossi; Quan-Wen Jin; Jun-Song Chen; Kathleen L. Gould ABSTRACT Cellular polarization underlies many facets of cell behavior, including cell growth. The rod-shaped fission yeast Schizosaccharomyces pombe is a well-established, genetically tractable system for studying...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (13): jcs244863.
Published: 07 July 2020
...Li Sun; Xiao-Min Liu; Wen-Zhu Li; Yuan-Yuan Yi; Xiangwei He; Yamei Wang; Quan-Wen Jin ABSTRACT In the fission yeast Schizosaccharomyces pombe , both RNAi machinery and RNAi-independent factors mediate transcriptional and posttranscriptional silencing and heterochromatin formation. Here, we show...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (11): jcs240234.
Published: 08 June 2020
... between microtubule motors and non-motor proteins. Defects in spindle assembly or changes in spindle dynamics are associated with diseases, such as cancer or developmental disorders. Here, we compared mitotic and meiotic spindles in fission yeast. We show that, even though mitotic and meiotic spindles...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (1): jcs240754.
Published: 08 January 2020
... to introduce heterologous sequences. Finally, as proof of concept, we generate a large set of ready-to-use, fluorescent probes to mark organelles and cellular processes with a wide range of applications in fission yeast research. This article has an associated First Person interview with the first author...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (22): jcs235911.
Published: 14 November 2019
...Helena Cantwell; Paul Nurse ABSTRACT Nuclear size scales with cell size across a wide range of cell types. The mechanism by which this scaling is maintained in growing cells remains unclear. Here, we investigate the mechanism of nuclear size homeostasis in the simple eukaryote fission yeast...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (19): jcs236133.
Published: 10 October 2019
...Susumu Morigasaki; Lit Chein Chin; Tomoyuki Hatano; Midori Emori; Mika Iwamoto; Hisashi Tatebe; Kazuhiro Shiozaki ABSTRACT Sin1 is a substrate-binding subunit of target of rapamycin complex 2 (TORC2), an evolutionarily conserved protein kinase complex. In fission yeast, Sin1 has also been...
Includes: Supplementary data
Journal Articles
In collection:
Cytoskeleton
J Cell Sci (2019) 132 (18): jcs232306.
Published: 23 September 2019
... of fission yeast kinesin-8 (Klp5-Klp6) and XMAP215/chTOG (Dis1) to study their individual and overlapping roles. We found that the non-motor kinesin-8 tailbox is essential for mitotic function; mutation compromises plus-end-directed processivity. Klp5-Klp6 induces catastrophes to control microtubule length...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (13): jcs227447.
Published: 01 July 2019
... that lipids may regulate CR assembly and function. Although there is clear evidence that phosphoinositides play an important role in cytokinesis, much less is known about the role of sterols in this process. Here, we studied how sterols influence division plane positioning and CR assembly in fission yeast. We...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (12): jcs230722.
Published: 25 June 2019
...Taisuke Seike; Hiromi Maekawa; Taro Nakamura; Chikashi Shimoda ABSTRACT In the fission yeast Schizosaccharomyces pombe , the mating reaction is controlled by two mating pheromones, M-factor and P-factor, secreted by M- and P-type cells, respectively. M-factor is a C-terminally farnesylated lipid...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (10): jcs229021.
Published: 15 May 2019
... acid elongase Elo2 is located in the nuclear membrane and prevents lethal defects associated with nuclear membrane ruptures in mutants of the nuclear membrane proteins Lem2 and Bqt4 in the fission yeast Schizosaccharomyces pombe. Lipid composition analysis shows that t20:0/24:0 phytoceramide...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (7): jcs223925.
Published: 03 April 2019
... and cell survival with the nutrient environment of cells. The poor vasculature and nutritional stress experienced by cells in solid tumours raises the question: how do they assimilate sufficient nutrients to survive? Here, we show that human and fission yeast cells import ATP and AMP from their external...
Includes: Supplementary data
Journal Articles
J Cell Sci (2018) 131 (16): jcs217331.
Published: 21 August 2018
... characterizations of fission yeast temperature-sensitive (ts) mutants of the evolutionarily conserved transmembrane protein Cwh43, and explore its relevance to utilization of glucose, nitrogen source and lipids. GFP-tagged Cwh43 localizes at ER associated with the nuclear envelope and the plasma membrane...
Includes: Supplementary data
Journal Articles
J Cell Sci (2018) 131 (15): jcs212167.
Published: 06 August 2018
... as conformational changes of proteins ( Geeves and Gutfreund, 1982 ; Pearson et al., 2008 ). High pressure (>200 bar) is lethal to most prokaryotic and eukaryotic cells. We used the genetically tractable fission yeast model system and this simple pressure chamber to study the impact pressure has upon...
Includes: Supplementary data
Journal Articles
In collection:
Polarity
J Cell Sci (2018) 131 (14): jcs216580.
Published: 19 July 2018
...Ye Dee Tay; Marcin Leda; Andrew B. Goryachev; Kenneth E. Sawin ABSTRACT The conserved Rho-family GTPase Cdc42 plays a central role in eukaryotic cell polarity. The rod-shaped fission yeast Schizosaccharomyces pombe has two Cdc42 guanine nucleotide exchange factors (GEFs), Scd1 and Gef1, but little...
Includes: Supplementary data