...Yusuke Toyoda; Fumie Masuda; Shigeaki Saitoh ABSTRACT In the fission yeast Schizosaccharomyces pombe , transcriptional upregulation and cell-surface localization of the hexose transporter Ght5 are required for cell proliferation in low glucose. As the target of rapamycin complex 2 (TORC2) signaling...

...Michiko Suma; Orie Arakawa; Yuria Tahara; Kenichi Sajiki; Shigeaki Saitoh; Mitsuhiro Yanagida ABSTRACT The evolutionarily conserved Mis4 protein establishes cohesion between replicated sister chromatids in vegetatively proliferating cells. In the fission yeast, Schizosaccharomyces pombe , defects...

...Xiao-Hui Lyu; Yu-Sheng Yang; Zhao-Qian Pan; Shao-Kai Ning; Fang Suo; Li-Lin Du ABSTRACT The ability to manipulate gene expression is valuable for elucidating gene function. In the fission yeast Schizosaccharomyces pombe , the most widely used regulatable expression system is the nmt1 promoter...

.... Fission yeast Phosphatidylinositol (4,5)-bisphosphate Phosphatidylinositol 4-phosphate 5-kinase ER–PM contacts Cytokinesis Cytokinetic ring National Institutes of Health http://dx.doi.org/10.13039/100000002 R35GM131799 Vanderbilt University http://dx.doi.org/10.13039/100006537...

... factors found in animals and fungi. In the fission yeast Schizosaccharomyces pombe , they regulate various cellular processes, including cell cycle progression, lipid metabolism and cell adhesion. CSL proteins bind to DNA through their N-terminal Rel-like domain and central β-trefoil domain. Here, we...

..., cellular responses to multiple stresses remain largely unknown. Here, we characterized fission yeast cellular responses to a novel stress inducer, non-thermal atmospheric-pressure plasma. Plasma irradiation generates ultraviolet radiation, electromagnetic fields and a variety of chemically reactive species...

... al., 2005 ; Field et al., 2005 ; Snider et al., 2017 , 2018 ). The fission yeast, Schizosaccharomyces pombe , has three PI-4-kinases: Stt4, Lsb6 and Pik1, and two PI4 phosphatases, Sac11 and Sac12 ( Fig. 1A ). Stt4 is an essential type IIIα enzyme that localizes to the PM via two scaffolds...

... the maintenance of INM homeostasis remain poorly understood. In this study, we explored the degradation mechanisms of the INM protein Bqt4 in the fission yeast Schizosaccharomyces pombe . We have previously shown that Bqt4 interacts with the transmembrane protein Bqt3 at the INM and is degraded in the absence...

... distribution of Pkd2 by promoting its internalization and preventing its clustering in the eisosome, a caveolae-like membrane compartment. The domains of Pkd2 and their functions are conserved in other fission yeast species. We conclude that both extracellular and cytoplasmic domains of Pkd2 are crucial...

... repressed state essential for cell survival in stressful environments. In the fission yeast Schizosaccharomyces pombe , cell size is reduced by cell division before entry into stationary phase; thus cyclin-dependent kinase (CDK) must actively contribute to stationary phase establishment. However...

... in the fission yeast Schizosaccharomyces pombe . However, recently we implemented a systematic and less time-consuming knockdown method, CRISPR interference (CRISPRi), in this organism using catalytically inactive Cas9 (dCas9). This technology has been expected to facilitate characterization of essential genes...

.... Here, we report the successful implementation of the CRISPR-Cas13d system in fission yeast for RNA knockdown. A high effectiveness of the CRISPR-Cas13d system was ensured by using an array of CRISPR RNAs (crRNAs) that are flanked by two self-cleaving ribozymes and are expressed from an RNA polymerase...

... microtubules via microtubule plus-end-directed motility. However, kinesin-5 localization is enhanced away from antiparallel overlaps. Increasing evidence suggests this localization occurs due to bidirectional motility or trafficking. The purified fission-yeast kinesin-5 protein Cut7 moves bidirectionally...

... of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Summary: The genes of the fission yeast ecl family respond to various forms...

... CENP-S–CENP-X is involved in regulating the SAC and kinetochore–microtubule attachments has not been well characterized. Using live-cell microscopy and yeast genetics, we found that Mhf1–Mhf2, the CENP-S–CENP-X counterpart in the fission yeast Schizosaccharomyces pombe , plays crucial roles...

... membrane-localized fission yeast polycystin homolog Pkd2p is an essential protein required for cytokinesis; however, its role remains unclear. Here, we isolated a novel temperature-sensitive pkd2 mutant, pkd2-B42 . Among the strong growth defects of this mutant, the most striking was that many mutant cells...

.... In the fission yeast, Schizosaccharomyces pombe , Rec8-containing meiotic cohesin is suggested to establish kinetochore associations by mediating cohesion of the centromere cores. However, cohesin-mediated kinetochore associations on intact chromosomes have never been demonstrated directly. In the present study...

... biliverdin (BV) as a chromophore. Here, we report that phycocyanobilin (PCB) functions as a brighter chromophore for iRFP than BV, and that biosynthesis of PCB allows live-cell imaging with iRFP in the fission yeast Schizosaccharomyces pombe . We initially found that fission yeast cells did not produce BV...

... show that the fission yeast S6 kinase Psk1 contains a TOS motif that interacts with Mip1, a RAPTOR ortholog. The TOS motif in Psk1 resembles those in mammals, including the conserved phenylalanine and aspartic acid residues essential for the Mip1 interaction and TORC1-dependent phosphorylation of Psk1...

...Zhong-Qiu Yu; Xiao-Man Liu; Dan Zhao; Dan-Dan Xu; Li-Lin Du ABSTRACT Protein–protein interactions are vital for executing nearly all cellular processes. To facilitate the detection of protein–protein interactions in living cells of the fission yeast Schizosaccharomyces pombe , here we present...