...Stephen M. Coscia; Cameron P. Thompson; Qing Tang; Elana E. Baltrusaitis; Joseph A. Rhodenhiser; Omar A. Quintero-Carmona; E. Michael Ostap; Melike Lakadamyali; Erika L. F. Holzbaur ABSTRACT Mitochondrial homeostasis requires a dynamic balance of fission and fusion. The actin cytoskeleton promotes...

... it is established that impairments in mitochondrial fusion and fission impact the mitochondrial genome and can lead to mtDNA depletion, abnormal nucleoid organization or accumulation of deletions, it is not entirely clear how or why remodeling mitochondrial network morphology affects mtDNA. Here, we focus on recent...

...Manuela Lavorato; Emanuele Loro; Valentina Debattisti; Tejvir S. Khurana; Clara Franzini-Armstrong ABSTRACT Mitochondria respond to stress and undergo fusion and fission at variable rates, depending on cell status. To understand mitochondrial behavior during muscle fatigue, we investigated...

... supplying this compartment with cargo receptors to be sorted ( Bennett, 1995 ; Söllner, 1995 ). Once cargo has been sorted into EE microdomains, these membrane regions bud into tubulo-vesicular structures that subsequently undergo fission and are transported along microtubule tracks to their target...

..., this leads to various physiological defects, including embryonic lethality, neurodegeneration and overt inflammation. The reasons for such drastic lysosome enlargement remain unclear. Here, we examined whether biosynthesis and/or fusion-fission dynamics contribute to swelling. First, we show that PIKfyve...

... of fusion and fission, mitochondrial homeostasis remains incompletely understood. Recent studies implicate dynamin-2 and dynamin-related protein-1 (Drp1, also known as DNM1L), as GTPases involved in mitochondrial fission. Here, we identify the ATPase and endocytic protein EHD1 as a novel regulator...

...Laura D. Osellame; Abeer P. Singh; David A. Stroud; Catherine S. Palmer; Diana Stojanovski; Rajesh Ramachandran; Michael T. Ryan ABSTRACT Cytosolic dynamin-related protein 1 (Drp1, also known as DNM1L) is required for both mitochondrial and peroxisomal fission. Drp1-dependent division...

... annular gap junction vesicles are formed. These vesicles undergo fission, but the molecular machinery needed for these fissions has not been described. The mechanoenzyme dynamin has been previously demonstrated to play a role in gap junction plaque internalization. To investigate the role of dynamin...

...Akinori Itoyama; Masanori Honsho; Yuichi Abe; Ann Moser; Yumi Yoshida; Yukio Fujiki Peroxisome division is regulated by several factors, termed fission factors, as well as the conditions of the cellular environment. Over the past decade, the idea of metabolic control of peroxisomal morphogenesis...

... dynamics, which is described as the balance between mitochondrial fusion and fission, has revealed that functional and structural alterations in mitochondrial morphology are important factors in several key pathologies associated with aging. Indeed, a recent wave of studies has demonstrated the pleiotropic...

..., proteins of the membrane fission machinery (Dnm1-Mdv1-Caf4-Fis1 in yeast and DLP1-FIS1 in human) have been shown to have a dual localization on mitochondria and peroxisomes, where they control mitochondrial fission and peroxisome division. Here, we show that whereas vacuole fusion is regulated...

... mitochondrial structure to its functional states have not been fully characterized. In this report, we analyze the bidirectional relationships between mitochondrial morphology and function in living human cells. First, we determined the effect of mitochondrial fission on energy production by using small...

...Tianzheng Yu; Randall J. Fox; Lindsay S. Burwell; Yisang Yoon Mitochondrial fission is a highly regulated process mediated by a defined set of protein factors and is involved in the early stage of apoptosis. In mammals, at least two proteins, the dynamin-like protein DLP1/Drp1 and the mitochondrial...

... in (a) the redistribution of mitochondria to the nuclear periphery, and (b) the formation of long and highly branched mitochondrial structures. Suggesting that an alteration in the balance between mitochondrial fission and fusion may be involved in both of these changes, overexpression of p50 induced the translocation...

... of mitochondria. We propose that Mfn1 T109A interferes with fusion activity of endogenous Mfn1 protein, possibly by binding necessary cofactors,so to allow unopposed mitochondrial fission. Thus, Mfn1 appears to be a key player in mediating mitochondrial fusion and morphology in mammalian cells. References...