... mitochondrial and peroxisomal fission (EMPF1) – a devastating neurodevelopmental disease with no effective treatment. To interrogate the mechanisms by which DRP1 mutations cause cellular dysfunction, we used human-derived fibroblasts from patients who present with EMPF1. In addition to elongated mitochondrial...

... and with extracellular matrix deposition during regeneration and repair. Interestingly, the expression of PDGFRα changed dynamically in the fibroblast lineage after injury. Furthermore, PDGFRα-dependent immediate early gene expression changed during regeneration and repair. We also found that TGF-β signaling reduces...

...Salma Jalal; Shidong Shi; Vidhyalakshmi Acharya; Ruby Yun-Ju Huang; Virgile Viasnoff; Alexander D. Bershadsky; Yee Han Tee ABSTRACT Actin cytoskeleton self-organization in two cell types, fibroblasts and epitheliocytes, was studied in cells confined to isotropic adhesive islands. In fibroblasts...

... pregnancy and lactation, epithelial spherical alveoli develop towards the ends of ducts, which synthesise milk and secrete it into the ductal network. Surrounding the ducts and alveoli is stromal tissue, comprising ECM components as well as fibroblasts, adipocytes, immune cells and endothelial cells...

... fibroblasts. Screen hits were validated on physiological compliance hydrogels, and selected hits tested in primary fibroblasts from patients with idiopathic pulmonary fibrosis. Our RNAi screen led to the identification of STAT3 as an essential mediator of myofibroblast activation and function. Strikingly, we...

... in response to TGFB1, leading to renal failure. Here, we define a novel interaction between the SET9 lysine methyltransferase (also known as SETD7) and SMAD3, the principal mediator of TGFB1 signalling in myofibroblasts. We show that SET9-deficient fibroblasts exhibit globally altered gene expression profiles...

... and thicker skin on the palms and soles is regulated by underlying fibroblasts in those areas, specifically via a secreted factor (DKK1) that modulates Wnt signaling. In this study, we tested the hypothesis that dermal fibroblasts regulate the constitutive skin color of individuals ranging from very light...

.... * Present address: Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA ‡ Author for correspondence ( ms2001@columbia.edu ) 18 11 2009 © 2010. 2010 Actin Cell spreading Coherence Fibroblast Myosin-II Traction force...

... of California San Diego, La Jolla, CA 92093, USA 28 1 2009 © The Company of Biologists Limited 2009 2009 Fibronectin Adhesion Fibroblast Shear Detachment Fibrillar matrix The extracellular matrix (ECM), an interconnected fibrous network of proteins and carbohydrates, serves...

..., as seen in fibroblasts, whereas a more rapid cycling will lead to a more snail-like shape. In addition, this model suggests why membrane ruffling occurs and that large non-circulating surface molecules move towards the back of the cell where they might assist in detaching the back end of the cell...

... senescence-associated (SA)-β-galactosidase staining, increased G1 arrest and delayed cell growth in human fibroblasts. Western blotting studies demonstrated that PPARγ activation can upregulate the expression of p16 INK4α . PPARγ can bind to the p16 promoter and induce its transcription, and, after treatment...

...Pinak S. Acharya; Sonali Majumdar; Michele Jacob; James Hayden; Paul Mrass; Wolfgang Weninger; Richard K. Assoian; Ellen Puré CD44 contributes to inflammation and fibrosis in response to injury. As fibroblast recruitment is critical to wound healing, we compared cytoskeletal architecture...

... by many cells, including keratinocytes and fibroblasts in the skin. In the past, α 2 β 1 integrin was suggested to be responsible for cell attachment, spreading and migration on monomeric collagen I and contraction of three-dimensional collagen lattices. In view of these functions, normal development...

..., but to inhibited cell migration. Although undetected in cells of non-migratory tissues as well as in confluent cultured cells, S1 fibers were found in all migratory cells examined, such as cultured cells in scratch/wound experiments, blood neutrophils and monocytes, and fibroblasts engaging in tissue healing...

... time that biglycan and decorin induce morphological and cytoskeletal changes in fibroblasts, resulting in an increase in migration. Biglycan changed the cell shape of fibroblasts with formation of long protruding filamentous processes. This was also seen for decorin but to a lesser extent. Using...

...-fold and Akt protein was partly phosphorylated. A similar shape change was also observed in nocodazole-treated L929 fibroblasts which were incubated with the similar phospholipid analogues. In those nucleated cells, where the microtubule cytoskeleton was disrupted, a major actin-dependent membrane...

...Elina M. Levina; Margarita A. Kharitonova; Yuri A. Rovensky; Jury M. Vasiliev In order to understand the factors determining the length of fibroblasts, three cell lines (mouse embryonic fibroblasts plus human fibroblast lines AGO 1523 and M19) were cultivated on the usual planar substrate (glass...

... shown to regulate the phenotype of vascular smooth muscle cells via αv and β1 integrins. In the current study we have examined whether vitronectin influences the phenotype and function of normal human lung fibroblasts (HFL-1). Incubation of HFL-1 cells with vitronectin induced a concentration-dependent...

...Kevin S. Ko; Pamela D. Arora; Vinay Bhide; Austin Chen; Christopher A. G. McCulloch ABSTRACT In connective tissues, intercellular adhesion is essential for tissue morphogenesis, development and wound healing. However, the signaling mechanisms initiated by cell-cell adhesion in fibroblasts...

...Qin M. Chen; Victoria C. Tu; Jeffrey Catania; Maggi Burton; Olivier Toussaint; Tarrah Dilley ABSTRACT Early passage human diploid fibroblasts develop senescent morphology prematurely within a week after a 2-hour pulse treatment with low or mild dose H 2 O 2 . We test here the role of cell cycle...