... body pathway, thus constituting an additional source of K63-polyubiquitylation beyond Rsp5/NEDD4 in the endocytic compartment. FYVE domain K63-polyubiquitylation Membrane protein sorting RING finger Ubiquitin protein ligase Ubiquitin-conjugating enzyme Post-translational modification...

... endosomes to regulate trafficking through this pathway. * These authors contributed equally to this work ‡ Author for correspondence ( christina.mitchell@med.monash.edu.au ) 10 6 2010 © 2010. 2010 MTMR4 Myotubularin Recycling endosomes Tfn FYVE domain The endocytic...

... members, MTMR3 contains a C-terminal FYVE domain. We show that the FYVE domain of MTMR3 is atypical in that it neither confers endosomal localisation nor binds to the lipid PtdIns3 P . Furthermore the FYVE domain is not required for in vitro enzyme activity of MTMR3. In contrast, the PH-GRAM domain...

...Li-Fong Seet; Wanjin Hong TOM1 and its related proteins, TOM1-like1 (TOM1-L1) and TOM1-like2 (TOM1-L2), constitute a subfamily of the VHS domain protein family. We have recently shown that endofin, a FYVE domain protein associated with the early endosome, is able to recruit cytosolic TOM1 onto...

... effects of PtdIns(3) P in these processes, it is important to identify PtdIns(3) P -binding proteins, many of which contain FYVE zinc-finger domains. Here, we describe a novel giant FYVE-domain-containing protein, named autophagy-linked FYVE protein (Alfy). Alfy is ubiquitously expressed, shares sequence...

...-kinases and FYVE domain proteins, such as EEA1, can be recruited onto early endosomes and act as Rab5 effectors. SARA (Smad anchor for receptor activation), also a FYVE domain protein, was initially isolated as a participant in signal transduction from the transforming growth factorβ receptor...

... proteins containing one or two FYVE-domains respectively. We show that the FYVE domains in these proteins can bind PtdIns3P in vitro with high specificity over other phosphoinositides. Exogenously expressed FENS-1 localises to early endosomes: this localisation requires an intact FYVE domain...

..., including an N-terminal VHS domain, a phosphatidylinositol 3-phosphate (PtdIns(3)P)-binding FYVE domain and two coiled-coil domains. Here we have investigated the roles of these domains in the subcellular localisation of Hrs. Hrs was found to colocalise extensively with EEA1, an established marker of early...