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Keywords: FRET
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Journal Articles
Series: REVIEW COMMONS TRANSFER
J Cell Sci (2022) 135 (20): jcs260627.
Published: 13 October 2022
... identify S146 as a phosphorylation switch defining ARP2/3 interaction and cancer cell invasion. FRET Invasion LUBAC MDA-MB-231 Integrin Phosphorylation Academy of Finland http://dx.doi.org/10.13039/501100002341 Syöpäsäätiö http://dx.doi.org/10.13039/501100010711...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2020) 133 (5): jcs234922.
Published: 5 March 2020
... of the living organism. Advanced FRET probes allow tracking signaling events in live cells. Light-sheet microscopy allows for deeper tissue penetration in optically clear samples, enriching our understanding of the higher-level organization of the immune response. Super-resolution microscopy offers insights...
Journal Articles
J Cell Sci (2020) 133 (2): jcs233064.
Published: 23 January 2020
...-FRET and FLIM-FRET. PIP 3 -mediated association with the cytoplasmic membrane and non-phosphorylated Y426 are required for SWAP-70 to dimerize and to bind F-actin. The dimerization region was identified near the C terminus where R546 is required for dimerization and, thus, F-actin bundling...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (20): jcs229906.
Published: 22 October 2019
... neurons from McGill-R-Thy1-APP transgenic rats, a model of Alzheimer's disease-like amyloid pathology. Live FRET imaging of neurons from transgenic rats revealed perturbed ‘lipid-MERCs’ (gap width <10 nm), while ‘Ca 2+ -MERCs’ (10–20 nm gap width) were unchanged. In situ TEM showed no significant...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2019) 132 (16): jcs230334.
Published: 23 August 2019
...Sonal Prasad; Evgeni Ponimaskin; Andre Zeug ABSTRACT Protein–protein interaction is often investigated using quantitative molecular microscopy with Förster resonant energy transfer (FRET). Here, we combined ‘linear unmixing FRET’ (lux-FRET) with the simultaneous application of a FRET-based...
Includes: Supplementary data
Journal Articles
Journal Articles
J Cell Sci (2018) 131 (6): jcs206730.
Published: 22 March 2018
... in a yeast-two hybrid screen of a cardiac library. We corroborated this interaction by performing Förster resonance energy transfer (FRET) in HEK293 cells and co-immunoprecipitation in HEK293 cells and native cardiac tissue. TRIOBP-1 overexpression reduced hERG surface expression and current density, whereas...
Includes: Supplementary data
Journal Articles
Journal Articles
Journal Articles
J Cell Sci (2016) 129 (2): 329–340.
Published: 15 January 2016
... a newly developed FRET sensor. A combination of a Rab7 sensor and confocal FRET imaging techniques revealed that the activation of Rab7 on late endosomes depends on Mon1–Ccz1 and is implicated in late-endosome–lysosome fusion. In contrast, Rab7 activity on lysosomes was independent of Mon1–Ccz1 and active...
Includes: Supplementary data
Journal Articles
Journal Articles
J Cell Sci (2014) 127 (24): 5253–5260.
Published: 15 December 2014
.... Nevertheless, the dynamics of phosphatidylinositol-(3,4,5)-trisphosphate (PIP 3 ) and the regulation of its synthesis and degradation at synaptic compartments is far from clear. Here, we have used fluorescence resonance energy transfer (FRET) imaging to monitor changes in PIP 3 levels in dendritic spines from...
Includes: Supplementary data
Journal Articles
In collection:
Lipid biology
J Cell Sci (2014) 127 (11): 2565–2576.
Published: 1 June 2014
... they bind effectors. Previous studies have suggested that the binding of Rac1 to membranes requires, and colocalizes with, cholesterol-rich liquid-ordered ( lo ) membrane domains (lipid rafts). Here, we have developed a fluorescence resonance energy transfer (FRET) assay that robustly detects Rac1 membrane...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (10): 2145–2150.
Published: 15 May 2014
...). Using high-resolution microscopic imaging, we find that cyclin A2 persists beyond metaphase. Indeed, we identify a novel cyclin-A2-containing compartment that forms dynamic foci. Förster (or fluorescence) resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) analyses show...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (9): 1899–1910.
Published: 1 May 2014
... and bind to specific DNA sequences, resulting in target gene expression. We analysed and compared the structural organizations of the unphosphorylated latent and phosphorylated activated STAT3 dimers using Förster resonance energy transfer (FRET) in fixed and living cells. The latent dimers are stabilized...
Journal Articles
J Cell Sci (2014) 127 (9): 2040–2052.
Published: 1 May 2014
... hair cells than in outer hair cells, and began as stereocilia fusion and depletion, accompanied by a thinning and waving circumferential actin belt at apical junctional complexes (AJCs). Adenovirus-encoded GFP–Cdc42 expression in hair cells and fluorescence resonance energy transfer (FRET) imaging...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (6): 1151–1160.
Published: 15 March 2014
... ( Day and Davidson, 2009 ; Sample et al., 2009 ; Shaner et al., 2005 ) enabled the construction of FRET-based biosensors, with changes in FRET actively reporting on biochemical events in cells. The first such biosensor, the Ca 2+ probe Cameleon, featured CaM and the M13 peptide sandwiched between...
Journal Articles
Journal Articles
J Cell Sci (2013) 126 (18): 4286–4295.
Published: 15 September 2013
... © 2013. Published by The Company of Biologists Ltd 2013 Islet β-cells Apoptosis Necrosis Live-cell imaging Fluorescence resonance energy transfer FRET For cell death assays, MIN6 and dispersed islet cells were seeded into 96-well plates and infected with lentiviral particles...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (13): 2914–2923.
Published: 1 July 2013
... changes in Syx by FRET. We explored the recent crystal structure of Syx in order to rationalize our design of the probes. In the available structure, the N-terminus of Syx and its juxtamembrane region, connecting the transmembrane anchor and the H3 domain, are in proximity in the ‘closed’ rather than...
Includes: Supplementary data