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Keywords: FRET
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Journal Articles
In collection:
Imaging
J Cell Sci (2020) 133 (5): jcs234922.
Published: 05 March 2020
... of the living organism. Advanced FRET probes allow tracking signaling events in live cells. Light-sheet microscopy allows for deeper tissue penetration in optically clear samples, enriching our understanding of the higher-level organization of the immune response. Super-resolution microscopy offers insights...
Journal Articles
J Cell Sci (2020) 133 (2): jcs233064.
Published: 23 January 2020
...-FRET and FLIM-FRET. PIP 3 -mediated association with the cytoplasmic membrane and non-phosphorylated Y426 are required for SWAP-70 to dimerize and to bind F-actin. The dimerization region was identified near the C terminus where R546 is required for dimerization and, thus, F-actin bundling...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (20): jcs229906.
Published: 22 October 2019
... neurons from McGill-R-Thy1-APP transgenic rats, a model of Alzheimer's disease-like amyloid pathology. Live FRET imaging of neurons from transgenic rats revealed perturbed ‘lipid-MERCs’ (gap width <10 nm), while ‘Ca 2+ -MERCs’ (10–20 nm gap width) were unchanged. In situ TEM showed no significant...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2019) 132 (16): jcs230334.
Published: 23 August 2019
...Sonal Prasad; Evgeni Ponimaskin; Andre Zeug ABSTRACT Protein–protein interaction is often investigated using quantitative molecular microscopy with Förster resonant energy transfer (FRET). Here, we combined ‘linear unmixing FRET’ (lux-FRET) with the simultaneous application of a FRET-based...
Includes: Supplementary data
Journal Articles
J Cell Sci (2018) 131 (13): jcs215541.
Published: 04 July 2018
... to, respectively, regulate pseudopod protrusion and ectodomain shedding during transendothelial migration. FRET FLIM Ectodomain shedding Polarity Transmigration Invasion An essential feature of acute inflammation is the migration of circulating innate immune cells, such as monocytes, towards...
Includes: Supplementary data
Journal Articles
J Cell Sci (2018) 131 (6): jcs206730.
Published: 22 March 2018
... in a yeast-two hybrid screen of a cardiac library. We corroborated this interaction by performing Förster resonance energy transfer (FRET) in HEK293 cells and co-immunoprecipitation in HEK293 cells and native cardiac tissue. TRIOBP-1 overexpression reduced hERG surface expression and current density, whereas...
Includes: Supplementary data
Journal Articles
J Cell Sci (2018) 131 (5): jcs206995.
Published: 06 March 2018
... imaging approaches in mice are reviewed with a focus on cancer progression and treatment, where it offers detailed insight into the dynamic interactions between cancer cells and their microenvironment in vivo . Intravital imaging Subcellular imaging FRAP FRET Biosensors Cancer Immune cells...
Journal Articles
J Cell Sci (2016) 129 (6): 1128–1140.
Published: 15 March 2016
... of adequate amplitude to activate chloride secretion. Cystic fibrosis Correctors cAMP PKA Airway cells FRET The most prevalent mutation of the CFTR gene associated with cystic fibrosis is the deletion of phenylalanine at residue 508 (F508del CFTR) in the nucleotide-binding domain 1 (NBD1...
Journal Articles
J Cell Sci (2016) 129 (2): 329–340.
Published: 15 January 2016
... a newly developed FRET sensor. A combination of a Rab7 sensor and confocal FRET imaging techniques revealed that the activation of Rab7 on late endosomes depends on Mon1–Ccz1 and is implicated in late-endosome–lysosome fusion. In contrast, Rab7 activity on lysosomes was independent of Mon1–Ccz1 and active...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (18): 3489–3501.
Published: 15 September 2015
... and a reduction in the number of functional channels. FRET KCNQ2 Src Gating Open probability M-channels are members of the Kv7 family of voltage-dependent K + channels and are expressed predominantly in neuronal cells, with the most abundant subunits being KCNQ2 (Q2) and KCNQ3 (Q3) ( Brown...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (24): 5253–5260.
Published: 15 December 2014
.... Nevertheless, the dynamics of phosphatidylinositol-(3,4,5)-trisphosphate (PIP 3 ) and the regulation of its synthesis and degradation at synaptic compartments is far from clear. Here, we have used fluorescence resonance energy transfer (FRET) imaging to monitor changes in PIP 3 levels in dendritic spines from...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (11): 2565–2576.
Published: 01 June 2014
... they bind effectors. Previous studies have suggested that the binding of Rac1 to membranes requires, and colocalizes with, cholesterol-rich liquid-ordered ( lo ) membrane domains (lipid rafts). Here, we have developed a fluorescence resonance energy transfer (FRET) assay that robustly detects Rac1 membrane...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (10): 2145–2150.
Published: 15 May 2014
...). Using high-resolution microscopic imaging, we find that cyclin A2 persists beyond metaphase. Indeed, we identify a novel cyclin-A2-containing compartment that forms dynamic foci. Förster (or fluorescence) resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) analyses show...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (9): 1899–1910.
Published: 01 May 2014
... and bind to specific DNA sequences, resulting in target gene expression. We analysed and compared the structural organizations of the unphosphorylated latent and phosphorylated activated STAT3 dimers using Förster resonance energy transfer (FRET) in fixed and living cells. The latent dimers are stabilized...
Journal Articles
J Cell Sci (2014) 127 (9): 2040–2052.
Published: 01 May 2014
... hair cells than in outer hair cells, and began as stereocilia fusion and depletion, accompanied by a thinning and waving circumferential actin belt at apical junctional complexes (AJCs). Adenovirus-encoded GFP–Cdc42 expression in hair cells and fluorescence resonance energy transfer (FRET) imaging...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (6): 1151–1160.
Published: 15 March 2014
.... , Sumiyama   K. , Mizuno   R. , Matsuda   M. ( 2013 ). Live imaging of transgenic mice expressing FRET biosensors.   Conf. Proc. IEEE Eng. Med. Biol. Soc.   2013 , 125 – 128 . Karginov   A. V. , Ding   F. , Kota   P. , Dokholyan   N. V. , Hahn   K. M. ( 2010 ). Engineered...
Journal Articles
J Cell Sci (2013) 126 (19): 4527–4535.
Published: 01 October 2013
... of these CaM-binding regions, we generated a fluorescence resonance energy transfer (FRET) pair, a dual CFP- and YFP-labeled RyR2 (RyR2 R3595-CFP/K4269-YFP ) with CFP inserted after Arg3595 and YFP inserted after Lys4269. We transfected HEK293 cells with the RyR2 R3595-CFP/K4269-YFP cDNA, and examined...
Journal Articles
J Cell Sci (2013) 126 (18): 4286–4295.
Published: 15 September 2013
... © 2013. Published by The Company of Biologists Ltd 2013 Islet β-cells Apoptosis Necrosis Live-cell imaging Fluorescence resonance energy transfer FRET Functional β-cell mass is maintained by a balance between β-cell growth, survival, proliferation, differentiation and programmed...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (13): 2914–2923.
Published: 01 July 2013
... to catalyze exocytosis. To better understand Syx conformational changes associated with the exocytotic process in a living cell, we constructed double-labeled fluorescent Syx probes that may report conformational changes in Syx by FRET. We explored the recent crystal structure of Syx in order to rationalize...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (11): 2401–2410.
Published: 01 June 2013
...+ influx. Here, we describe novel inter- and intramolecular FRET sensors in the context of the full-length membrane-anchored STIM1, and show that STIM1 undergoes a conformational change in response to store depletion to adopt a stretched ‘open’ conformation that exposes SOAR/CAD and allows it to interact...