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Keywords: FRAP
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Journal Articles
J Cell Sci (2022) 135 (14): jcs259629.
Published: 26 July 2022
... more rapidly but dissolved more slowly. Looking specifically at an mRNA and at some protein components of SGs using FRAP showed that cells that received cortisone in addition to VRB showed higher immobile fractions of G3BP1 and IG2BP3 proteins in the SG structures compared to those of cells treated...
Includes: Supplementary data
Journal Articles
J Cell Sci (2022) 135 (2): jcs258818.
Published: 26 January 2022
... fluorescence recovery after photobleaching (FRAP) using the SMC4 homolog DPY-27 and showed that a well-characterized ATPase mutation abolishes DPY-27 binding to X chromosomes. Next, we performed FRAP in the background of several chromatin modifier mutants that cause varying degrees of X chromosome derepression...
Includes: Supplementary data
Journal Articles
In collection:
Membrane Trafficking
J Cell Sci (2021) 134 (8): jcs254185.
Published: 27 April 2021
... to the maturing endosome, which is necessary for the convergence phase of Rab5 maturation. Formation of the initial Rab5 domains in the convergence phase might be spontaneous and GTP dependent, as found in cell-free studies ( Cezanne et al., 2020 ). Our FRAP experiments showed a gradual increase of the relative...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (4): jcs234682.
Published: 26 February 2020
... recovery in the absence of latrunculin B treatment. To determine the degree to which myosin XIa binds to VAMP72-labeled vesicles , we conducted FRAP experiments in the presence of latrunculin B ( Fig. 2 A). By removing the actin cytoskeleton, we ensure that myosin XIa fluorescence recovery...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (15): jcs228338.
Published: 7 August 2019
...-1-1/ Summary: The dynamics of the evolutionarily conserved polarity regulator Crumbs at the plasma membrane is important for modulating junctional stability and tension during embryonic development of Drosophila . Apical Moesin β H -spectrin FERM protein FRAP Morphogenesis...
Includes: Supplementary data
Journal Articles
Journal Articles
J Cell Sci (2018) 131 (5): jcs206995.
Published: 6 March 2018
... these hurdles. As discussed above, intravital FRAP and FRET imaging can provide pivotal insights into the subcellular regulation of protein dynamics and activity. However, these approaches are limited by the optical restrictions of confocal laser scanning and multiphoton microscopy, including magnification...
Journal Articles
In collection:
Imaging
J Cell Sci (2016) 129 (17): 3342–3350.
Published: 1 September 2016
... recovery after photobleaching FRAP Obstructed diffusion Membrane trafficking Plasma membrane lipids and proteins are enriched within areas of the plasma membrane to form patches of different sizes ( Vereb et al., 2003 ; Engelman, 2005 ). There are likely different mechanisms for patch formation...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (22): 4160–4170.
Published: 15 November 2015
... and transport carrier formation. By performing fluorescence recovery after photobleaching (FRAP) and mathematical modeling, we show that p150 glued (also known as DCTN1), a component of the dynactin complex, stabilizes the COPII pre-budding complex on ER membranes in a microtubule-independent manner...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (18): 3435–3443.
Published: 15 September 2015
..., we used knock-out mouse embryonic fibroblasts re-expressing different vinculin mutants in traction microscopy, magnetic tweezer microrheology, FRAP and actin-binding assays. Compared to cells expressing wild-type or constitutively active vinculin, we found reduced tractions, cytoskeletal stiffness...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (20): 4409–4419.
Published: 15 October 2014
... Polarity Neuron Migration Membrane traffic FRAP Neurons are extremely polarized cells, with axons specialized in delivering electrical signals that trigger the release of neurotransmitters from presynaptic contacts, and dendrites specialized in sensing neurotransmitters through specific...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (16): 3440–3450.
Published: 15 August 2014
... photobleaching (FRAP) experiments reveal that CasSD phosphorylation, as opposed to the association of Cas with Src, facilitates Cas displacement from adhesion complexes in migrating cells. Furthermore, the stabilization of Src–Cas binding and inhibition of myosin II, both of which sustain CasSD phosphorylation...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (15): 3320–3330.
Published: 1 August 2014
... recovery after photobleaching (FRAP) and mEos2 photoactivation to monitor the dynamics of IMC biogenesis and turnover during the replication of Toxoplasma gondii tachyzoites. These studies reveal that the formation of the T. gondii IMC involves two distinct steps – de novo assembly during daughter IMC...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (7): 1406–1416.
Published: 1 April 2014
... interests The authors declare no competing interests. 23 05 2013 31 12 2013 © 2014. Published by The Company of Biologists Ltd 2014 Steroid Receptor Transcription factor DNA binding Single-molecule microscopy FCS FRAP The androgen receptor (AR) is a ligand-activated...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (9): 2092–2101.
Published: 1 May 2013
... photobleaching (FRAP) of GFP-tagged α 1 and β subunits expressed in dysgenic myotubes to study the relative dynamics of these Ca 2+ channel subunits for the first time in a native functional signaling complex. Identical fluorescence recovery rates of both subunits indicate stable interactions, distinct recovery...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (6): 1429–1439.
Published: 15 March 2013
... proteins for regulating BiP binding of clients in living cells. FRAP analysis revealed that overexpressed ERdj3-sfGFP dramatically decreases BiP-GFP mobility in a client-dependent manner. By contrast, ERdj3-GFP mobility remains low regardless of client protein levels. Native gels and co...
Includes: Supplementary data
Journal Articles
Journal Articles
Journal Articles
J Cell Sci (2011) 124 (19): 3332–3343.
Published: 1 October 2011
... for GFP, and a 40 mW 561 nm diode laser with a 565 longpass filter for mRFP and tdTomato. FRAP experiments were performed by photobleaching an ROI at full laser power of the 489 nm line and monitoring fluorescence recovery over time. No photobleaching of the adjacent cells during the processes...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (19): 3254–3265.
Published: 1 October 2011
... of the bleached area was measured. Subtracting the background, normalizing by fluorescent decay during scan and exponential recovery curve fitting were performed using FRAP for ZEN software (Carl Zeiss Microimaging). The double exponential recovery curve is described by the equation, F ( t )=A1(1–e –K1t )+A2(1–e...
Includes: Supplementary data