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Keywords: FRAP
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Journal Articles
In collection:
Membrane Trafficking
J Cell Sci (2021) 134 (8): jcs254185.
Published: 27 April 2021
... endosomal maturation is the exchange of Rab5 with Rab7a, and we show that this two-phase exchange is finalized by the formation of Rab5-positive early endosomes. Endosomal biogenesis Endosome maturation FRAP Rab5 Rab7a Endosomal trafficking is carefully regulated through the mechanisms of...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (4): jcs234682.
Published: 26 February 2020
.... Published by The Company of Biologists Ltd 2020 http://www.biologists.com/user-licence-1-1/ Summary: Myosin XI rapidly associates and dissociates with vesicles and F-actin. This turnover is predicted to be essential for cell polarization and growth. FRAP Intracellular transport Myosin V...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (15): jcs228338.
Published: 07 August 2019
.../user-licence-1-1/ Summary: The dynamics of the evolutionarily conserved polarity regulator Crumbs at the plasma membrane is important for modulating junctional stability and tension during embryonic development of Drosophila . Apical Moesin β H -spectrin FERM protein FRAP...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (8): jcs224493.
Published: 25 April 2019
... sterically affects the recruitment of paxillin to the integrin–talin–kindlin complex. In this regard, the phenotype of the β1D integrin P786A mutation is interesting. On the one hand, the P786A mutation in β1D integrin affects the stability of the integrin within adhesions (as demonstrated using FRAP), while...
Includes: Supplementary data
Journal Articles
J Cell Sci (2018) 131 (5): jcs206995.
Published: 06 March 2018
...: Intravital imaging approaches in mice are reviewed with a focus on cancer progression and treatment, where it offers detailed insight into the dynamic interactions between cancer cells and their microenvironment in vivo . Intravital imaging Subcellular imaging FRAP FRET Biosensors Cancer Immune...
Journal Articles
In collection:
Imaging
J Cell Sci (2016) 129 (17): 3342–3350.
Published: 01 September 2016
... recovery after photobleaching FRAP Obstructed diffusion Membrane trafficking Plasma membrane lipids and proteins are enriched within areas of the plasma membrane to form patches of different sizes ( Vereb et al., 2003 ; Engelman, 2005 ). There are likely different mechanisms for patch formation...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (22): 4160–4170.
Published: 15 November 2015
... and transport carrier formation. By performing fluorescence recovery after photobleaching (FRAP) and mathematical modeling, we show that p150 glued (also known as DCTN1), a component of the dynactin complex, stabilizes the COPII pre-budding complex on ER membranes in a microtubule-independent manner...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (18): 3435–3443.
Published: 15 September 2015
... activation, we used knock-out mouse embryonic fibroblasts re-expressing different vinculin mutants in traction microscopy, magnetic tweezer microrheology, FRAP and actin-binding assays. Compared to cells expressing wild-type or constitutively active vinculin, we found reduced tractions, cytoskeletal...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (20): 4409–4419.
Published: 15 October 2014
... Polarity Neuron Migration Membrane traffic FRAP Neurons are extremely polarized cells, with axons specialized in delivering electrical signals that trigger the release of neurotransmitters from presynaptic contacts, and dendrites specialized in sensing neurotransmitters through specific...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (16): 3440–3450.
Published: 15 August 2014
... photobleaching (FRAP) experiments reveal that CasSD phosphorylation, as opposed to the association of Cas with Src, facilitates Cas displacement from adhesion complexes in migrating cells. Furthermore, the stabilization of Src–Cas binding and inhibition of myosin II, both of which sustain CasSD phosphorylation...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (15): 3320–3330.
Published: 01 August 2014
..., fluorescence recovery after photobleaching (FRAP) and mEos2 photoactivation to monitor the dynamics of IMC biogenesis and turnover during the replication of Toxoplasma gondii tachyzoites. These studies reveal that the formation of the T. gondii IMC involves two distinct steps – de novo assembly during daughter...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (7): 1406–1416.
Published: 01 April 2014
... influenced our view of the organization of nuclear processes. Approaches like fluorescence recovery after photobleaching (FRAP) ( Van Royen et al., 2009 ) and fluorescence correlation spectroscopy (FCS) ( Weidtkamp-Peters et al., 2009 ) have provided novel insights into the mechanism of action of nuclear...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (9): 2092–2101.
Published: 01 May 2013
... photobleaching (FRAP) of GFP-tagged α 1 and β subunits expressed in dysgenic myotubes to study the relative dynamics of these Ca 2+ channel subunits for the first time in a native functional signaling complex. Identical fluorescence recovery rates of both subunits indicate stable interactions, distinct recovery...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (6): 1429–1439.
Published: 15 March 2013
... proteins for regulating BiP binding of clients in living cells. FRAP analysis revealed that overexpressed ERdj3-sfGFP dramatically decreases BiP-GFP mobility in a client-dependent manner. By contrast, ERdj3-GFP mobility remains low regardless of client protein levels. Native gels and co...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (19): 4498–4506.
Published: 01 October 2012
... ventral and peripheral cellular position of the FAs. * These authors contributed equally to this work ‡ Author for correspondence ( b.water@lacdr.leidenuniv.nl ) 28 5 2012 © 2012. Published by The Company of Biologists Ltd 2012 Focal adhesion FRAP FLIP We thank...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (4): 869–886.
Published: 15 February 2012
... the cytoskeleton and controlling the association by producing cAMP, to yield a highly organized signalling hub. * Author for correspondence ( dmfc2@cam.ac.uk ) 26 9 2011 © 2012. 2012 Adenylyl cyclase cAMP Cytoskeleton Cholesterol FRAP Rafts Given the similarity in...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (19): 3332–3343.
Published: 01 October 2011
.... * Author for correspondence ( erik-lee.snapp@einstein.yu.edu ) 17 5 2011 © 2011. 2011 BiP ERAD FRAP Huntingtin UPR The ability of a cell to detect and respond to various stresses is crucial for cell survival. To cope with misfolded protein accumulation, cells have developed...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (19): 3254–3265.
Published: 01 October 2011
... © 2011. 2011 Chromatin FRAP Histone chaperone Linker histone H1 Nuclear dynamics TAF-I Eukaryotic genomic DNA is associated with chromatin proteins including histones and non-histone proteins, which form the chromatin structure. The repeating unit of chromatin is a nucleosome core...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (17): 2877–2890.
Published: 01 September 2011
... always available in vivo. Collectively, these methods present a paradigm shift towards the molecular-level investigation of disease and therapy in animal models of disease. * Author for correspondence ( k.anderson@beatson.gla.ac.uk ) © 2011. 2011 In vivo imaging Intravital imaging FRAP...
Journal Articles
J Cell Sci (2011) 124 (10): 1703–1714.
Published: 15 May 2011
... with transcripts containing only five CUG repeats. Fluorescence recovery after photobleaching (FRAP) experiments showed that CUG-repeat RNAs display a stochastic aggregation behaviour, because individual RNA foci formed at different rates and displayed different recoveries. Spontaneous clustering of...
Includes: Multimedia, Supplementary data