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Keywords: FRAP
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Journal Articles
J Cell Sci jcs.259629.
Published: 17 June 2022
... and reproduction in any medium provided that the original work is properly attributed. FRAP Live-cell imaging Stress Stress granules European Research Council https://dx.doi.org/10.13039/501100000781 GENEXP Israel Science Foundation https://dx.doi.org/10.13039/501100003977 2252...
Journal Articles
J Cell Sci (2022) 135 (2): jcs258818.
Published: 26 January 2022
... fluorescence recovery after photobleaching (FRAP) using the SMC4 homolog DPY-27 and showed that a well-characterized ATPase mutation abolishes DPY-27 binding to X chromosomes. Next, we performed FRAP in the background of several chromatin modifier mutants that cause varying degrees of X chromosome derepression...
Includes: Supplementary data
Journal Articles
In collection:
Membrane Trafficking
J Cell Sci (2021) 134 (8): jcs254185.
Published: 27 April 2021
... that this two-phase exchange is finalized by the formation of Rab5-positive early endosomes. Endosomal biogenesis Endosome maturation FRAP Rab5 Rab7a Endosomal trafficking is carefully regulated through the mechanisms of endosomal progression. In general, two models have been characterized...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (4): jcs234682.
Published: 26 February 2020
... analytically for simple boundary conditions, no analytical solution exists for our cell shape. We have previously shown, through extensive experimentation and simulations of FRAP, that cell shape is important to model FRAP ( Bibeau et al., 2018 ; Kingsley et al., 2018 ). Here, we used the finite element...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (15): jcs228338.
Published: 7 August 2019
...-1-1/ Summary: The dynamics of the evolutionarily conserved polarity regulator Crumbs at the plasma membrane is important for modulating junctional stability and tension during embryonic development of Drosophila . Apical Moesin β H -spectrin FERM protein FRAP Morphogenesis...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (8): jcs224493.
Published: 25 April 2019
... Paxillin Adhesion Focal adhesions Fibrillar adhesions Fibronectin FRAP Talin1 Talin2 Heterodimeric receptors of the integrin family play crucial roles in cell adhesion and signaling during development and tissue homeostasis in the adult. In addition to varying subunit compositions...
Includes: Supplementary data
Journal Articles
J Cell Sci (2018) 131 (5): jcs206995.
Published: 6 March 2018
... ) or respiration ( Lee et al., 2012 ). Image-based stabilisation can also be used to correct for lateral sample motion in post-processing ( Fiole et al., 2014 ; Lee et al., 2014 ; Soulet et al., 2013 ; Vercauteren et al., 2006 ; Vinegoni et al., 2014 ). Motion of cell–cell junctions during intravital FRAP...
Journal Articles
In collection:
Imaging
J Cell Sci (2016) 129 (17): 3342–3350.
Published: 1 September 2016
... these conditions. It is clear, however, that a non-random distribution of delivery sites will be a more favorable scenario for MHC-I patch formation. We used FRAP in order to compare patch distributions in the same large membrane area at two different time points, imaging patches, then ‘erasing’ the fluorescence...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (22): 4160–4170.
Published: 15 November 2015
... and transport carrier formation. By performing fluorescence recovery after photobleaching (FRAP) and mathematical modeling, we show that p150 glued (also known as DCTN1), a component of the dynactin complex, stabilizes the COPII pre-budding complex on ER membranes in a microtubule-independent manner...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (18): 3435–3443.
Published: 15 September 2015
..., we used knock-out mouse embryonic fibroblasts re-expressing different vinculin mutants in traction microscopy, magnetic tweezer microrheology, FRAP and actin-binding assays. Compared to cells expressing wild-type or constitutively active vinculin, we found reduced tractions, cytoskeletal stiffness...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (20): 4409–4419.
Published: 15 October 2014
... Polarity Neuron Migration Membrane traffic FRAP Neurons are extremely polarized cells, with axons specialized in delivering electrical signals that trigger the release of neurotransmitters from presynaptic contacts, and dendrites specialized in sensing neurotransmitters through specific...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (16): 3440–3450.
Published: 15 August 2014
... photobleaching (FRAP) experiments reveal that CasSD phosphorylation, as opposed to the association of Cas with Src, facilitates Cas displacement from adhesion complexes in migrating cells. Furthermore, the stabilization of Src–Cas binding and inhibition of myosin II, both of which sustain CasSD phosphorylation...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (15): 3320–3330.
Published: 1 August 2014
... recovery after photobleaching (FRAP) and mEos2 photoactivation to monitor the dynamics of IMC biogenesis and turnover during the replication of Toxoplasma gondii tachyzoites. These studies reveal that the formation of the T. gondii IMC involves two distinct steps – de novo assembly during daughter IMC...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (7): 1406–1416.
Published: 1 April 2014
... et al., 2005 ; Mikuni et al., 2007a ; Mikuni et al., 2007b ) and, recently, the group of McNally ( Stasevich et al., 2010 ) cross-validated FRAP and FCS measurements on glucocorticoid receptor dynamics. Although the findings obtained with the two approaches were relatively consistent, uncertainties...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (9): 2092–2101.
Published: 1 May 2013
... photobleaching (FRAP) of GFP-tagged α 1 and β subunits expressed in dysgenic myotubes to study the relative dynamics of these Ca 2+ channel subunits for the first time in a native functional signaling complex. Identical fluorescence recovery rates of both subunits indicate stable interactions, distinct recovery...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (6): 1429–1439.
Published: 15 March 2013
... proteins for regulating BiP binding of clients in living cells. FRAP analysis revealed that overexpressed ERdj3-sfGFP dramatically decreases BiP-GFP mobility in a client-dependent manner. By contrast, ERdj3-GFP mobility remains low regardless of client protein levels. Native gels and co...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (19): 4498–4506.
Published: 1 October 2012
... of a 60-mW argon laser at 488 nm. Recovery of fluorescence within the strip was monitored using 100-ms intervals and low laser intensity to avoid photobleaching by monitoring. Approximately 10 cells were averaged to generate one FRAP curve for a single experiment. Student’s t -test was used...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (4): 869–886.
Published: 15 February 2012
... five independent transfections with the AC constructs. Clearly, the distribution and function of AC8 is tightly regulated by an association with actin as well as cholesterol. We felt that FRAP and fluorescence correlation spectroscopy (FCS) analysis would discern the quantitative underpinnings...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (19): 3332–3343.
Published: 1 October 2011
... was significantly lower than the level observed in cells expressing GFP alone that were treated with Tm. Thus, Htt ex1 expression does induce UPR in N2a cells. * Author for correspondence ( erik-lee.snapp@einstein.yu.edu ) 17 5 2011 © 2011. 2011 BiP ERAD FRAP Huntingtin UPR...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (19): 3254–3265.
Published: 1 October 2011
... FRAP Histone chaperone Linker histone H1 Nuclear dynamics TAF-I Eukaryotic genomic DNA is associated with chromatin proteins including histones and non-histone proteins, which form the chromatin structure. The repeating unit of chromatin is a nucleosome core particle (NCP) that consists...
Includes: Supplementary data