... network. Fluorescence lifetime imaging microscopy (FLIM)-FRET measurements indicate that the forces generated by NMII isoform B (NMIIB) exhibit significant spatial and temporal heterogeneity as a function of donor lifetime and fluorophore energy exchange. These measurements provide a proxy for inferred...

...-FRET and FLIM-FRET. PIP 3 -mediated association with the cytoplasmic membrane and non-phosphorylated Y426 are required for SWAP-70 to dimerize and to bind F-actin. The dimerization region was identified near the C terminus where R546 is required for dimerization and, thus, F-actin bundling...

... to, respectively, regulate pseudopod protrusion and ectodomain shedding during transendothelial migration. FRET FLIM Ectodomain shedding Polarity Transmigration Invasion Medical Research Council 10.13039/501100007155 MR/K50130X/1 Biotechnology and Biological Sciences Research...

...). Using high-resolution microscopic imaging, we find that cyclin A2 persists beyond metaphase. Indeed, we identify a novel cyclin-A2-containing compartment that forms dynamic foci. Förster (or fluorescence) resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) analyses show...

... (CDM). PDAC cells grown on CDM imaged in phase contrast (top) or using FLIM–FRET to show RhoA activity (bottom). The matrix can be seen as background striations in the phase-contrast image (top). Organotypic. H&E section (top) showing cell invasion from the top surface down into the matrix...

..., no differences in FRET efficiency were measured (data not shown). Since it is known that the ECFP and EYFP have the tendency to dimerize, the ECFP was replaced by a monomerized, brighter variant ( Kremers et al., 2006 ), to abolish intramolecular dimerization. FLIM was used to measure FRET efficiencies ( van...

... of Biologists Limited 2007 2007 FLIM FRET SNARE Exocytosis Every eukaryotic cell relies on the SNARE proteins to mediate the vesicular targeting, docking and fusion processes that underlie membrane trafficking and exocytosis. Essential to the exocytotic process are the interactions...