...Biao Yan; Ban Liu; Chang-Dong Zhu; Kang-Le Li; Li-Jia Yue; Jin-Liang Zhao; Xiao-Ling Gong; Cheng-Hui Wang Summary MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in numerous biological processes. However, the role of miRNAs in skin color determination in fish has...

... genome (supplementary material Fig. S6A). These probes label 16SrRNAs and several tRNAs and mRNAs, and they do not recognize regions involved in the initiation of DNA replication (see below). FISH experiments with mTRANS, in the absence or presence of nucleases, confirmed that this probe set detects only...

... termed AML -BCR3), which does not contain any repetitive sequences, was used as a probe for fluorescent in situ hybridization (FISH) with nuclear halos (prepared from HEL cells as described in Materials and Methods). It should be mentioned that, although HEL cells originate from a human...

... and transfected cell lines. These in vivo integration events provided a system to study the association and integration patterns of each introduced S/MAR. By utilizing FISH to visualize directly the localization of S/MARs on the nuclear matrix or chromatin loop, we were able to assign specific attributes to the S...

...Jeffrey M. Levsky; Robert H. Singer Fluorescence in situ hybridization (FISH), the assay of choice for localization of specific nucleic acids sequences in native context, is a 20-year-old technology that has developed continuously. Over its maturation,various methodologies and modifications have...

...) 24 7 2002 © The Company of Biologists Limited 2002 2002 Yeast Hybrid Zygote Nuclear architecture FISH Interphase Meiosis Chromosome Hybrid organisms often show increased vigour over their parental species but they usually trade in fertility for the high degree...

... Nuclear architecture GFP Pairing FISH Mitosis The distribution of chromosomes within interphase nuclei is not random. First, in most species there exists a polarized arrangement of chromosome arms, the so-called Rabl orientation, where centromeres are assembled near one pole of the nucleus...

... mutants of rye (Secale cereale L.) by means of fluorescence in situ hybridization (FISH). Homozygotes for sy1 and sy9 non-allelic mutations form axial elements during leptotene of male meiosis, but fail to form synaptonemal complexes. Consequently, recombination is severely impaired, and high univalency...

... two-dimensional projections of FISH signals, several spermatogonia were subjected to light optical serial sectioning. In 11 serially sectioned spermatogonia (n =13), homologous chromosome territories were of variable shape and spatially separate (Figs 5a,b , 6a,b). Chromosome arm domains were detected...

... 11 1996 09 12 1966 © 1997 by Company of Biologists 1997 FISH Nuclear localization Polyadenylation Pre-mRNA splicing Transcription Before being transported to the cytoplasm, most pre-mRNAs of higher eukaryotes undergo a number of modifications that include capping...

...%), frog (71%), chick and human (73% each). Large stretches in the central αhelical rod are identical within all four classes of vertebrates, but in 17 residues spread over the entire rod, the two fish differ distinctly from the tetrapod species. In addition, in the more diverged non-helical head domain...

... trout, Salmo gairdneri Richardson , spermatozoa: Effects of and pH on motility . J. Fish Biol . 19 , 259 – 267 . 10.1111/j.1095-8649.1981.tb05830.x Billard , R. (1969). Ultrastructure comparée de spermatozoïdes de quelques poissons téléostéens . In Quaderno, n°137. Acc. Naz. dei...