... of R546A should be attributed to its impaired dimerization. To test this further, we labelled purified R546A mutant with the thiol-reactive dye Atto 655 and measured its diffusion time using fluorescence correlation spectroscopy (FCS), which is similar to FCCS approach described above except that only one...

... our view of the organization of nuclear processes. Approaches like fluorescence recovery after photobleaching (FRAP) ( Van Royen et al., 2009 ) and fluorescence correlation spectroscopy (FCS) ( Weidtkamp-Peters et al., 2009 ) have provided novel insights into the mechanism of action of nuclear...

.... The nucleus is a dynamic compartment and live-cell imaging techniques make it possible to investigate transcription factor action in real-time. We studied the diffusion of EGFP–RAR by fluorescence correlation spectroscopy (FCS) to uncover the molecular interactions determining receptor mobility...

... are determined by a combination of endocytic accessory proteins and the cholesterol content of different membrane compartments. * Author for correspondence ( [email protected] ) 3 8 2009 © The Company of Biologists Limited 2009 2009 FCS SBD Microdomain Raft Sphingolipid...

... leukemia SP100 SUMO RARα Assembly Kinetics modeling FRAP FCS Beside chromosome territories, the mammalian nucleus contains a variety of internal domains, such as nucleoli, speckles, Cajal bodies and promyelocytic leukemia nuclear bodies (PML NBs) ( Lamond and Earnshaw, 1998...

..., which specifically bind PTS2-containing peptides. * Author for correspondence (e-mail: [email protected] ) 28 4 2005 Yeast PTS2 protein import Peroxisomes FCS Hansenula polymorpha Peroxisomes are membrane-bound organelles, present in virtually all eukaryotic...