... revealed that its C-terminal splice variant ATP11C-b exhibits polarized localization in motile cell lines, such as MDA-MB-231 and Ba/F3. In the present study, we found that the C-terminal cytoplasmic region of ATP11C-b interacts specifically with ezrin. Notably, the LLxY motif in the ATP11C-b C-terminal...

...Kévin Leguay; Barbara Decelle; Yu Yan He; Anthony Pagniez; Mireille Hogue; Hiroyuki Kobayashi; Christian Le Gouill; Michel Bouvier; Sébastien Carréno ABSTRACT Ezrin, radixin and moesin compose the family of ERM proteins. They link actin filaments and microtubules to the plasma membrane to control...

... of membrane required for the formation of the phagocytic pseudopodia is maintained by cortical ezrin, and that its cleavage is a key step in releasing this membrane, the cleavage of cortical ezrin was monitored within living phagocytes (the phagocytically competent cell line RAW264.7) through expressing two...

... trafficking of AQP2 relies on its close interaction with trafficking machinery proteins and the actin cytoskeleton. Here, we report the identification of ezrin, an actin-binding protein from the ezrin/radixin/moesin (ERM) family as an AQP2-interacting protein. Ezrin was first detected in a co...

...Abass Al-Momany; Laiji Li; R. Todd Alexander; Barbara J. Ballermann ABSTRACT CLIC5A (encoded by CLIC5 ) is a component of the ezrin–NHERF2–podocalyxin complex in renal glomerular podocyte foot processes. We explored the mechanism(s) by which CLIC5A regulates ezrin function. In COS-7 cells, CLIC5A...

... specific AKAPs are depleted by siRNA-mediated knockdown, point to ezrin as a scaffold required for hCG-, cAMP- and PKA-mediated regulation of the fusion process. By a variety of immunoprecipitation and immunolocalization experiments, we show that ezrin directs PKA to a molecular complex of connexin 43...

...Andrea I. McClatchey ABSTRACT The cell cortex is a dynamic and heterogeneous structure that governs cell identity and behavior. The ERM proteins (ezrin, radixin and moesin) are major architects of the cell cortex, and they link plasma membrane phospholipids and proteins to the underlying cortical...

... of the activity of ezrin, a downstream target of Rap, with the glucosylceramide synthase inhibitor, miglustat, effectively inhibited cell spreading of Slp2-a-knockdown cells. We also discovered aberrant expression of Slp2-a and increased activity of ezrin in pcy ( Nphp3 pcy ) mice, a model of polycystic kidney...

...-signal transduction, we performed a small-scale siRNA screen in A549 lung epithelial cells. Of the Rap effectors tested, only Radil blocked Rap-induced spreading. Additionally, we identified a novel role for Ezrin downstream of Rap1. Ezrin was necessary for Rap-induced cell spreading, but not Rap-induced...

... in membrane blebbing. Unlike elongated A375 melanoma cells, rounded A375 cells do not display any obvious morphological front–back polarisation, although polarisation is thought to be a prerequisite for cell movement. We show that blebbing A375 cells are polarised, with ezrin (a linker between the plasma...

... by the nuclear factor κB (NF-κB) is essential, because inhibition of signaling by the inhibitor of κB super repressor (IκB-SR) blocked L1-mediated metastasis. Overexpression of the NF-κB p65 subunit was sufficient to increase CRC cell proliferation, motility and metastasis. Binding of the L1 cytodomain to ezrin...

...Dominique Lallemand; Aurelie Lampin Saint-Amaux; Marco Giovannini Merlin is the product of the Nf2 tumor-suppressor gene, and inactivation of Nf2 leads to the development of neural tumors such as schwannomas and meningiomas in humans and mice. Merlin is a member of the ERM (ezrin, radixin...

..., while actin-binding proteins such as ezrin and moesin direct binding of signaling molecules to actin filaments within the distal pole complex. Upon encountering an antigen-presenting cell (APC), a T cell undergoes dramatic structural changes. Migration ceases, the uropod retracts, and the leading edge...

...Ester Martín-Villar; Diego Megías; Susanna Castel; Maria Marta Yurrita; Senén Vilaró; Miguel Quintanilla Podoplanin is a small membrane mucin expressed in tumors associated with malignant progression. It is enriched at cell-surface protrusions where it colocalizes with members of the ERM (ezrin...

... that proteomics is a valuable tool for analysis of early development. * Author for correspondence (e-mail: [email protected] ) 8 2 2006 © The Company of Biologists Limited 2006 2006 Proteomics Ezrin Gastrulation Zebrafish The systematic analysis of gene transcription...

... of foxj1 -/- mice by immunohistochemistry. No change was detected in the expression of calpain 2 in the pulmonary epithelium by western blot or immunohistochemistry. By western blot and immunofluorescence, ezrin, a substrate for calpain, was also found to decrease in the pulmonary epithelium of foxj1...

...Casey M. Finnerty; David Chambers; Janet Ingraffea; H. Richard Faber; P. Andrew Karplus; Anthony Bretscher Members of the ezrin-radixin-moesin (ERM) protein family serve as regulated microfilament-membrane crosslinking proteins that, upon activation, bind the scaffolding protein ERM-phosphoprotein...

... of Biologists Limited 2004 2004 CD44 Hyaluronan ERM Ezrin Phosphorylation Proteolytic cleavage Hyaluronan is a ubiquitous glycosaminoglycan component of the extracellular matrix in vertebrates. It consists of large linear polymers of repeating disaccharide units often exceeding 4 MDa...

... glycoproteins N-cadherin and cadherin-11 [which also occur in heterotypic (`mixed') complexes] are associated with α- and β-catenin, plakoglobin (proportions variable among species), p120 ctn and vinculin. The other complex contains ezrin, periplakin, periaxin and desmoyokin (and so is called the EPPD complex...

... of those genes necessary for implementation of the invasion programme. Two such gene products, CD44 and ezrin, are both upregulated in fibroblasts transformed by v- fos and are commonly implicated in cell motility and invasion. Here we report that CD44 and ezrin colocalise to membrane ruffles...