...Nadja Hümpfer; Ria Thielhorn; Helge Ewers ABSTRACT Expansion microscopy (ExM) is a revolutionary novel approach to increase resolution in light microscopy. In contrast to super-resolution microscopy methods that rely on sophisticated technological advances, including novel instrumentation, ExM...

... Summary: To study the intricate metabolic network of sphingolipids, minimally modified probes are the tools of choice. We review progress made by using these probes together with the power of click chemistry. Expansion microscopy Lipid metabolism Lipid transport Photoactivation...

... restriction is the recently developed expansion microscopy (ExM). However, the original ultrastructure ExM (U-ExM) protocol is very time-consuming, and some epitopes are lost during the process. In this study, we developed a shortened pre-gelation staining ExM (PS-ExM) protocol and tested it to investigate...

... remain significant challenges, limiting the numbers of three-dimensional (3D) nanoscale processes that can be investigated and the number of laboratories able to undertake such analysis. Expansion microscopy (ExM) solves many of these limitations, but its application to imaging nuclear processes has been...