... ERK regulation in single intact cells, we find that, when protein kinase C or epidermal growth factor receptors are activated, a substantial fraction of the ERK nuclear localization response is uncoupled from TEY phosphorylation. This phosphorylation-unattributable nuclear localization response occurs...

... progenitor cell proliferation. Here, we investigated signaling pathways elicited by ADPβS, UTP and epidermal growth factor (EGF). All three agonists elicit ERK1/2 and CREB phosphorylation but the temporal characteristics differ between the nucleotides and EGF. Differentiation of the progenitors alters...

... factor receptor (EGFR) through a transactivation process. Healing of wounds in sheets of corneal epithelial cells is absolutely dependent on epidermal growth factor receptor signaling, and the present data suggest that its activation is a result of wound-induced phospholipase D activation. * Author...

... show that phosphatidylinositol 4-kinase IIα is also a component of highly dynamic membranes of the endosomal system where it colocalises with protein markers of the late endosome and with endocytosed epidermal growth factor. When phosphatidylinositol 4-kinase IIα activity was inhibited in vivo using...

... with early endocytic variants Rab4, Rab5, Rab17 and Rab22 but much less overlap with those associated with late endosomes, recycling endosomes and the early secretory pathway. Cells expressing Rab21 T33N had defects in endocytosis of transferrin and epidermal growth factor and failed to effectively deliver...

... with a PPARγ antagonist, GW9662, which attenuated the TZ-induced response in a dose-specific manner. The PPARγ-mediated effect on UP gene expression was maximal when there was concurrent inhibition of autocrine-activated epidermal growth factor receptor (EGFR) signalling through either the phosphatidylinositol...

... the lysosomal and ubiquitin-proteasome pathway. It was previously shown that the proteasome is involved in regulating the number of functional gap junctions at the plasma membrane. However, little is known about how proteasome-dependent turnover of Cx43 is controlled. Epidermal growth factor (EGF) induces...

... membrane. Although the plasma membrane-localized activity of Rab5 was not detected by light microscopy, overexpression of a GDP-bound mutant of CFP-Rab5(S34N) inhibited internalization of the epidermal growth factor receptor by retaining receptors in clathrin-coated pits. To test whether the Rab5(S34N...

.... Because growth factors are generally believed to regulate glial functions, we addressed their possible contribution to GLAST regulation in cultured rat astrocytes. Of the six growth factors tested (basic fibroblast growth factor (bFGF), insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF...

... influences these processes. The current study was performed to establish whether p190RhoGAP directly participates in epidermal growth factor-induced actin stress fiber disassembly and how c-Src is involved in this process. Our results support a model in which the p190 MD negatively regulates the activity...

... of the phospholipase was investigated in fibroblasts upon stimulation with epidermal growth factor and the calcium ionophore A23187. By the use of indirect immunofluorescence microscopy, staining of endogenous cytosolic phospholipase A 2 resulted in a punctate labeling pattern randomly distributed throughout...

...-independent mitogens, epidermal growth factor (EGF)+serum. EGF+serum also slightly inhibited the weak basal accumulation of p27 kip1 . Nevertheless, in the case of stimulation by TSH alone, the cAMP-dependent cell cycle progression was fully compatible with the enhanced expression of p27 kip1...

...Christina M. Van Itallie; Maria S. Balda; James Melvin Anderson ABSTRACT Addition of epidermal growth factor (EGF) to A431 human epidermal carcinoma cells results in actin reorganization and phosphorylation of several cytoskeletal proteins. In the present study, we found that EGF treatment...

... the molecules tested, epidermal growth factor (EGF) and transforming growth factor-α (TGF-α)were demonstrated to stimulate an increase in mammary epithelial cell motility and wound closure that was associated with a morphological transformation of the cells and was accompanied by modifications in cell-cell...

... 08 12 1992 27 01 1993 © 1992 by Company of Biologists 1992 PCNA immunocytochemical localization cell cycle thyrotropin epidermal growth factor cAMP thyroid The transition from quiescence to DNA synthesis in the eukaryotic cell cycle involves the synthesis of both...

...Philip J. Rijken; Willem J. Hage; Paul M. P. Van Bergen En Henegouwen; Arie J. Verkleiji; Johannes Boonstra ABSTRACT Double immunofluorescence microscopy reveals that epidermal growth factor (EGF) treatment of A43l cells results in more apparent co-localization of EGF receptor (EGFR) and actin...

...Pia Geimer; Ernesto G. Bade ABSTRACT Epidermal growth factor (EGF) is a potent mitogen for most cultured cells and has previously been shown to induce the migration of rat liver epithelial cells. We have now demonstrated that under migration-inducing conditions EGF does not stimulate cell...

...Wilhelm Engström ABSTRACT The effects of epidermal growth factor (EGF) on clones from a human embryonal carcinoma-derived cell line (Tera-2) have been studied. Cells were plated at clonal densities, whereafter the effects of serum and EGF on cell locomotion and cell proliferation were examined...

... on the intracellular sites in addition to cell surface sites. * Author for correspondence. 13 02 1986 09 04 1986 © 1986 by Company of Biologists 1986 internalization epidermal growth factor human placenta We have shown in the preceding paper that lysosomes, rough and smooth...

...N. Ramani; N. Chegini; Ch. V. Rao; P. G. Woost; G. S. Schultz ABSTRACT Highly purified lysosomes, rough and smooth endoplasmic reticulum, and Golgi apparatus, as well as microvillus plasma membranes, bound 125 I-labelled epidermal growth factor ([ 125 I]EGF) with similar affinity. Scatchard plots...