...Shaka X; Claire Aitken; Vedanta Mehta; Blanca Tardajos-Ayllon; Jovana Serbanovic-Canic; Jiayu Zhu; Bernadette Miao; Ellie Tzima; Paul Evans; Yun Fang; Martin A. Schwartz ABSTRACT Fluid shear stress (FSS) from blood flow, sensed by the vascular endothelial cells (ECs) that line all blood vessels...

...Jacob A. VanderBurgh; Archit V. Potharazu; Samantha C. Schwager; Cynthia A. Reinhart-King ABSTRACT Intimal stiffening upregulates endothelial cell contractility, disrupting barrier integrity; however, intimal stiffening is non-uniform. The impact of local changes in intimal stiffness on proximal...

...Camille Lenzi; Jennifer Stevens; Daniel Osborn; Matthew J. Hannah; Ruben Bierings; Tom Carter ABSTRACT Elevations of intracellular free Ca 2+ concentration ([Ca 2+ ] i ) are a potent trigger for Weibel–Palade body (WPB) exocytosis and secretion of von Willebrand factor (VWF) from endothelial cells...

... that it promotes angiogenic responses in endothelial cells, although the underlying signalling mechanisms are unclear. Reactive oxygen species derived from NADPH oxidases are major signalling molecules in the vasculature. Because NOX2 is functional in endothelial cells, we investigated the role of the NOX2...

... expressed on epithelial, hematopoietic and endothelial cells. CEACAM1 functions as an adhesion molecule, mainly binding to itself or other members of the CEA family. We and others have previously shown that CEACAM1 is crucial for in vivo vascular integrity during ischemic neo-vascularization. Here, we have...

... of cardiovascular development and vascular integrity. We investigated vascular endothelial growth factor (VEGF)-mediated ERK5 activation in primary human dermal microvascular endothelial cells (HDMECs) undergoing proliferation on a gelatin matrix, and tubular morphogenesis within a collagen gel matrix. VEGF induced...

... of ADMA is not well understood. We studied the role of exogenous and endogenous ADMA in the regulation of cell motility and actin cytoskeleton in porcine pulmonary endothelial cells (PAECs) and pulmonary microvascular endothelial cells (PMECs) from knockout mice that lack one of the enzyme metabolising...

... of endodermal, endothelial and hematopoietic markers, which contrasts with findings in the mouse in which TGFβ reduced the level of endodermal markers but increased endothelial marker expression. The inhibition observed was not due to changes in proliferation or apoptosis. This marked contrast between the two...

... complexus adherens junctions. These junctions are found at contact sites between endothelial cells that line capillaries. Few studies have focused on the function of DP in de novo capillary formation (vasculogenesis) and branching (angiogenesis) during tumorigenesis, embryonic development, cardiovascular...

... localisation of cPLA 2 -α in the EA.hy.926 human endothelial cell line was investigated. Nuclear localisation was dependent on proliferation, with subconfluent cells containing higher levels of nuclear cPLA 2 -α than contact-inhibited confluent or serum-starved cells. The broad-range protein kinase inhibitor...

...Marc A. Lafleur; Madeleine M. Handsley; Vera Knäuper; Gillian Murphy; Dylan R. Edwards Macro- and microvascular endothelial cells (EC) formed tubular structures when cultured within a 3D fibrin matrix, a process that was enhanced by vascular endothelial growth factor (VEGF), fibroblast growth...

... endothelial ephrin-B1 tyrosine phosphorylation, migration and integrin-mediated (α v β 3 andα 5 β 1 ) attachment and promoted neovascularization, in vivo, in a mouse corneal micropocket assay. Activation of ephrin-B1 by EphB1/Fc induced phosphorylation of p46 JNK but not ERK-1/2 or p38 MAPkinases. By contrast...

...Richard Hoffman; Dietrich H. Paper; Jane Donaldson; Susanne Alban; Gerhard Franz ABSTRACT We have evaluated a series of laminarin sulphates with different degrees of sulphation (0.3-2.3) as antagonists of basic fibroblast growth factor (bFGF) and as inhibitors of the bFGF-dependent endothelial cell...

...William D. Norris; John G. Steele; Graham Johnson; P. Anne Underwood ABSTRACT The initial attachment and spreading of endothelial cells from human umbilical artery onto type i collagen, type IV collagen or gelatin substrata was shown to be enhanced by inclusion of serum in the culture medium...