... infection and protein quality control. How SPP recognizes its various substrates and specifies their fate remains elusive. Here, we identify the lanosterol demethylase CYP51A1 as an SPP substrate and show that SPP-catalysed cleavage triggers CYP51A1 clearance by endoplasmic reticulum-associated degradation...

... transport is largely unexplored. To elucidate whether LIMP-2 is present at contact sites between lysosomes and the endoplasmic reticulum (ER), we performed a proximity-based interaction screen. This revealed that LIMP-2 interacts with the endosomal protein STARD3 and the ER-resident protein VAPB. Using...

...Suzan Kors; Anne-Lore Schlaitz ABSTRACT The endoplasmic reticulum (ER) is a dynamic and continuous membrane network with roles in many cellular processes. The importance and maintenance of ER structure and function have been extensively studied in interphase cells, yet recent findings also indicate...

... either to the endoplasmic reticulum (ER) or to the Golgi apparatus. However, the basis for differential sorting to the ER and to the Golgi apparatus remained unclear. To clarify this point, we quantitatively analyzed the intracellular targeting of a collection of proteins exhibiting a single TMD. Our...

...Peace Atakpa-Adaji; Adelina Ivanova; Karolina Kujawa; Colin W. Taylor ABSTRACT Inositol 1,4,5-trisphosphate (IP 3 ) receptors (IP 3 Rs) are high-conductance channels that allow the regulated redistribution of Ca 2+ from the endoplasmic reticulum (ER) to the cytosol and, at specialized membrane...

... expressing an mCherry–GFP-tagged Atg8a transgene exhibited aberrant persistence of the GFP signal within these aggregates, indicating failure of autophagosome maturation. These autophagy deficits were similar to those exhibited by loss of the endoplasmic reticulum (ER) fusion protein Atlastin (Atl), raising...

...Tai-Ting Woo; Jeffrey M. Williams; Billy Tsai ABSTRACT The multi-functional endoplasmic reticulum (ER) is exploited by viruses to cause infection. Morphologically, this organelle is a highly interconnected membranous network consisting of sheets and tubules whose levels are dynamic, changing...

... in the self-interaction and localization to the highly curved ER domains. Endoplasmic reticulum ER COPII ER exit sites Sec16 Sed4 Ministry of Education, Culture, Sports, Science and Technology http://dx.doi.org/10.13039/501100001700 18K06126 19K06655 17KT0105 21K06164...

... that Myo19 acts as a dynamic actin-binding tether that facilitates mitochondrial fragmentation. Myo19-driven fragmentation was blocked by depletion of either the CAAX splice variant of the endoplasmic reticulum (ER)-anchored formin INF2 or the mitochondrially localized F-actin nucleator Spire1C (a splice...

... as isomerase and reductase. Disulfide formation Endoplasmic reticulum Protein disulfide isomerase Protein folding Protein secretion Wellcome Trust http://dx.doi.org/10.13039/100010269 103720 University of Glasgow http://dx.doi.org/10.13039/501100000853...

... and HIG2 are targeted to LDs, and identify both ATGL-independent and -dependent mechanisms. Structural prediction and studies in cells reveal that ATGL-independent localization of G0S2 to both the endoplasmic reticulum (ER) and LDs is mediated by a hairpin structure consisting of two hydrophobic sequences...

.... , West , M. , Rowland , A. A. and Voeltz , G. K. ( 2013 ). Endoplasmic reticulum-endosome contact increases as endosomes traffic and mature . Mol. Biol. Cell 24 , 1030 - 1040 . 10.1091/mbc.e12-10-0733 Fu , M. M. and Holzbaur , E. L. ( 2014 ). Integrated regulation...

... in several cell compartments. In mammals, acetyl-CoA transporter 1 (AT1, also known as SLC33A1) facilitates the flux of cytosolic acetyl-CoA into the endoplasmic reticulum (ER), enabling the acetylation of proteins of the secretory pathway, in concert with the activity of dedicated acetyltransferases...

... frontotemporal dementia (ALS/FTD). Many of these changes can be linked to alterations in endoplasmic reticulum (ER)–mitochondria signaling, including dysregulation of Ca 2+ signaling, autophagy, lipid metabolism, ATP production, axonal transport, ER stress responses and synaptic dysfunction. ER–mitochondria...

... type I ALG genes Congenital disorders of glycosylation Endoplasmic reticulum Hypoglycosylation Lipid-linked oligosaccharide N -glycosylation Oligosaccharyltransferase Schizosaccharomyces pombe Yeast Agencia Nacional de Promoción Científica y Técnológica http://dx.doi.org...

...Imadeddin Hijazi; Emily Wang; Michelle Orozco; Sarah Pelton; Amy Chang ABSTRACT Endoplasmic reticulum stress (ERS) occurs when cellular demand for protein folding exceeds the capacity of the organelle. Adaptation and cell survival in response to ERS requires a critical contribution by mitochondria...

...Pawel Leznicki; Hayden O. Schneider; Jada V. Harvey; Wei Q. Shi; Stephen High ABSTRACT Membrane proteins destined for lipid droplets (LDs), a major intracellular storage site for neutral lipids, are inserted into the endoplasmic reticulum (ER) and then trafficked to LDs where they reside...

... by organelles inherited from the mothers. To determine whether this organelle inheritance occurs in a stereotyped manner, we tracked organelles using fluorescence microscopy. We describe a program for organelle inheritance in budding yeast. The cortical endoplasmic reticulum (ER) and peroxisomes are inherited...

... show that both proteins are attached to the cytosolic side of the endoplasmic reticulum membrane. Moreover, an interaction with cytosolic HSP70.1 and HSP90.2 could be shown using bimolecular fluorescence complementation. Knockout of both DJC31 and DJC62 caused severe defects in growth and development...

... pool, indicating a dynamic equilibrium between cytoplasmic and nuclear Dcp1/2. Cytoplasmic Dcp1/2 is linked to Dhh1 via Edc3. Functional P-bodies are present at the endoplasmic reticulum where Dcp2 potentially acts to increase the local concentration of Dhh1 through interaction with Edc3 to drive phase...