.../ Highlighted Article: Loss of Tetrahymena thermophila Poc5 reveals an important role for this centrin-binding protein in basal body maturation, which also impacts basal body production and ciliogenesis. Basal body Centriole Centrin Poc5 Tetrahymena Electron tomography National...

... vesicles (SVs) as well as rapid restoration of release sites. Here, we examined the role of the adaptor protein AP180 (also known as SNAP91) for IHC synaptic transmission by comparing AP180-knockout (KO) and wild-type mice using high-pressure freezing and electron tomography, confocal microscopy, patch...

... membrane architecture. Further, cryoAPEX is compatible with electron tomography. As an example, we apply cryoAPEX to obtain a high-resolution three-dimensional contextual map of the human FIC (filamentation induced by cAMP) protein, HYPE (also known as FICD). HYPE is a single-pass membrane protein...

... of information derived from light microscopy with rather few insights about spindle ultrastructure obtained from electron microscopy. In this Review, we will provide insights into the history of imaging of mitotic spindles and highlight recent technological advances in electron tomography and data processing...

.... To explore this possibility, we have examined neuronal subcellular architecture by performing three-dimensional (3D) electron tomography analysis of mouse brain tissue that had been preserved through high-pressure freezing. Here, we report that sequestration of neuronal cytoplasmic contents occurs...

... the reorganization of long trains at the flagellar tip. We demonstrate with electron tomography the existence of two distinct ultrastructural organizations for the short trains, we name these ‘narrow’ and ‘wide’, and provide the first 3D model of the narrow short trains. These trains are characterized by tri-lobed...

... species to laterally attach the flagellum to the cell body from the flagellar pocket to the cell posterior. Here, we use seven Leishmania mexicana cell lines that expressed eYFP fusions of FAZ protein homologs to show that the Leishmania flagellar pocket includes a FAZ structure. Electron tomography...

... 2013 7 02 2014 © 2014. Published by The Company of Biologists Ltd 2014 Cytostome Trypanosoma cruzi Endocytosis Electron tomography Endocytosis comprises a complex series of events that culminates in the internalization of extracellular molecules and particles. This process...

... overcomes the host cell cortical actin barrier to achieve the remarkably rapid process of internalization (less than a few seconds). Using correlative light and electron microscopy in conjunction with electron tomography and three-dimensional image analysis we identified that toxofilin, an actin-binding...

...Marlene Vinzenz; Maria Nemethova; Florian Schur; Jan Mueller; Akihiro Narita; Edit Urban; Christoph Winkler; Christian Schmeiser; Stefan A. Koestler; Klemens Rottner; Guenter P. Resch; Yuichiro Maeda; J. Victor Small Using correlated live-cell imaging and electron tomography we found that actin...

... known as choline-phosphate cytidylyltransferase A). This structure can form during interphase, confirming that it is independent of mitosis and therefore not a consequence of disordered nuclear envelope assembly. Serial-section dual-axis electron tomography reveals that these invaginations can take two...

... of filopodia. Live-cell imaging of fluorescently tagged proteins revealed that cofilin is specifically targeted to filopodia upon stalling of protrusion and during their retraction. Subsequent electron tomography established filopodial actin filament and/or bundle fragmentation to precisely correlate...

... and in vitro results has not been possible because MT end structures could not be correlated directly with the dynamic state of that particular MT. Here we combine light microscopy and electron tomography (ET) to show that growing MT plus ends in the fission yeast Schizosaccharomyces pombe display...

... is interfaced with morphogenesis of overall cell shape and form. The centriole or basal body of eukaryotic cells is often seen to be at the centre of such processes. We have used a combination of electron microscopy and electron tomography techniques to provide a detailed three-dimensional view of duplication...

...Sylvain Lacomble; Sue Vaughan; Catarina Gadelha; Mary K. Morphew; Michael K. Shaw; J. Richard McIntosh; Keith Gull This study uses electron tomography linked to a variety of other EM methods to provide an integrated view of the flagellar pocket and basal body area of the African trypanosome...

... cell plate. Here, we analyse the effects of the cell-plate assembly matrix on phragmoplast microtubule plus-end geometry by electron tomography of cryogenically fixed Arabidopsis meristem cells. Five distinct microtubules plus-end geometries are seen - blunt, extended, horned, flared and hybrid...

... by electron tomography. Isolated nucleoli, which exhibit a reduced transcriptional rate, were used to determine the sites of initial BrUTP incorporation (i.e. rRNA synthesis by the transcriptional machinery). Using pulse-chase experiments with BrUTP and an elongation inhibitor,cordycepin, it was possible...