.... Finally, we assess how recent advances in electron microscopy (EM), considered the gold standard for detecting MCSs, have allowed the visualization of MCSs and associated proteins in 3D at ever greater resolution. * Author for correspondence ( [email protected] ) Competing interests...

...Jens Wohlmann ABSTRACT The unparalleled resolving power of electron microscopy is both a blessing and a curse. At 30,000× magnification, 1 µm corresponds to 3 cm in the image and the field of view is only a few micrometres or less, resulting in an inevitable reduction in the spatial data available...

... and the accompanying poster, we review our current knowledge on the different types of canonical and non-canonical membrane-associated clathrin assemblies in mammalian cells, as observed by thin-section or platinum replica electron microscopy in various cell types, and discuss how the structural plasticity of clathrin...

... distance, as measured by electron microscopy, with ionic fluxes. We found that native cER cisternae extended during store depletion and remained elongated at a constant ER-plasma membrane (PM) gap distance during subsequent Ca 2+ elevations. Tethering proteins enhanced store-dependent cER expansion...

... of this process are intensively studied, while information about how the cell responds structurally to heat stress is mostly lacking. Here, Saccharomyces cerevisiae were subjected to a mild continuous heat shock (38°C) and intermittently cryo-immobilised for electron microscopy. Through measuring changes in all...

...) on a glow-discharged carbon-coated grid (Electron Microscopy Sciences, CF300-CU). For low-salt conditions, septins were polymerized by dilution into low-salt buffer and incubated for 1 h to overnight at room temperature before grid adsorption. In the case of GFP-labeled septins, septins were polymerized...

...Sandrine Pizette; Tamás Matusek; Bram Herpers; Pascal P. Thérond; Catherine Rabouille ABSTRACT In metazoans, tissue growth and patterning is partly controlled by the Hedgehog (Hh) morphogen. Using immuno-electron microscopy on Drosophila wing imaginal discs, we identified a cellular structure...

..., collectively called the ‘switchblade model’. This hypothesis, however, has not been extensively tested for other classes of integrins. We prepared a set of recombinant integrin ectodomain fragments including αvβ3, α2β1, α3β1, α5β1, α6β1 and α6β4, and used negative-stain electron microscopy to examine...

...Mark Terasaki ABSTRACT The endoplasmic reticulum (ER) is an interconnected network of tubules and sheets. In most tissues of the body, ER tubules have a diameter of ∼60 nm. Using new methods for serial-section electron microscopy, a distinct class of very narrow, 20- to 30-nm-diameter tubules were...

...Faye M. Nixon; Thomas R. Honnor; Nicholas I. Clarke; Georgina P. Starling; Alison J. Beckett; Adam M. Johansen; Julia A. Brettschneider; Ian A. Prior; Stephen J. Royle ABSTRACT Serial block face scanning electron microscopy (SBF-SEM) is a powerful method to analyze cells in 3D. Here, working...

... precluded structural analysis. Because it is difficult to capture rare and transient events, only a few attempts have been made to observe specific developmental and pathological processes in animal models using electron microscopy. The multimodal correlative approach that we propose here combines...

... channels in ER‐plasma membrane clusters. A longer splice variant, STIM1L, forms permanent ER‐plasma membrane clusters and mediates rapid Ca 2+ influx in muscle. Here, we used electron microscopy, total internal reflection fluorescence (TIRF) microscopy and Ca 2+ imaging to establish the trafficking...

... of Biologists Ltd 2015 Plasma membrane Lipid asymmetry Microdomain Electron microscopy Freeze fracture The asymmetric distribution of lipids between the outer and inner leaflet of the membrane bilayer was first reported in red blood cells (RBCs) ( Bretscher, 1972a ; Bretscher, 1972b...

... no competing or financial interests. © 2015. Published by The Company of Biologists Ltd 2015 Caveolae Caveolin Cavin Coiled coil Electron microscopy X-ray crystallography Caveolae (or ‘little caves’) are bulb-shaped invaginations of 50–60 nm that are a main feature of the plasma...

... extensively with traditional light and electron microscopy to determine the subcellular localization of lipids and their spatial and temporal regulation. We highlight some of the recent studies that have investigated the distribution of lipids and their ability to cluster using super-resolution and electron...

...Graham Knott; Christel Genoud Summary Since electron microscopy (EM) first appeared in the 1930s, it has held centre stage as the primary tool for the exploration of biological structure. Yet, with the recent developments of light microscopy techniques that overcome the limitations imposed...

... (3D) images of cellular structures. The ability to perform both fluorescence and electron microscopy on the same sample (correlative light and electron microscopy, CLEM) makes it possible to identify where a fluorescently labeled protein is located with respect to organelle structures visualized by EM...

... fundamental questions as to the biogenesis, subcellular distribution and inheritance of lipid droplets are as yet unsolved. In this study, we applied a set of imaging techniques such as high-resolution four-dimensional (4D) live-cell imaging, quantitative microscopy, transmission electron microscopy...

... preferentially transcribed a network of KLF1-regulated genes. Here we used correlative light microscopy in combination with energy filtering transmission electron microscopy (EFTEM) and electron microscopy in situ hybridization (EMISH) to analyse transcription factories, transcribing genes, and their nuclear...

... question is whether podosomes form protrusions similar to the invadopodia of cancer cells. We characterised podosomes of immature dendritic cells using electron microscopy combined with both conventional and novel high-resolution structured illumination light microscopy. Dendritic cell podosomes...