... apical trafficking. Furthermore, we show that both phospho-activated ERM (pERM; Ezrin-Radixin-Moesin) and Crb3.L are recruited to the growing apical domain of MCCs, where Crb3.L likely anchors pERM, allowing actin-dependent expansion of the apical membrane. Confocal images were acquired using ZEISS LSM...

...), NuMA (also known as NUMA1) and dynein–dynactin, plays a key role in establishing proper spindle orientation. It has also been shown that the actin-binding protein MISP and the ERM family, which are activated by lymphocyte-oriented kinase (LOK, also known as STK10) and Ste20-like kinase (SLK) (hereafter...

... and the block to polyspermy. Here, we show that Ano1 expression increases the oocyte surface, revealing a novel function for Ano1 in regulating cell morphology. Confocal imaging shows that Ano1 increases microvilli length, which requires ERM-protein-dependent linkage to the cytoskeleton. A dominant-negative...

... as proteins of the ERM family. We propose that PrP modulates β1 integrin adhesion and migration of monocytes through RhoA-induced actin remodeling mediated by cofilin, and through the regulation of ERM-mediated membrane–cytoskeleton linkage. References Alblas , J. , Ulfman , L. , Hordijk , P...

...Jarama Clucas; Ferran Valderrama ABSTRACT Members of the ezrin–radixin–moesin (ERM) family of proteins are involved in multiple aspects of cell migration by acting both as crosslinkers between the membrane, receptors and the actin cytoskeleton, and as regulators of signalling molecules...

.... In addition, SrcY527F was less able to induce podosome rosettes in JNK1-null or JNK2-null mouse embryo fibroblasts than in wild-type counterparts. The kinase activity of JNK was essential for promoting podosome rosette formation but not for its localization to podosome rosettes. Moesin, a member of the ERM...

... podocalyxin-1 at microvilli. Podocalyxin-1 recruits ezrin/radixin/moesin (ERM)-binding phosphoprotein-50 (EBP50; also known as NHERF1), which recruits ERM proteins and phosphatidylinositol 4-phosphate 5-kinase β (PIP5Kβ). Thus, clustering of PIP5Kβ leads to local accumulation of phosphatidylinositol (4,5...

... T cells and primary lymphocytes, we have investigated whether HIV-1 Env might promote viral entry and infection by activating ERM (ezrin-radixin-moesin) proteins to regulate F-actin reorganization and CD4/CXCR4 co-clustering. The interaction of the X4-tropic protein HIV-1 gp120 with CD4 augments...

...Grazia Tamma; Enno Klussmann; Johannes Oehlke; Eberhard Krause; Walter Rosenthal; Maria Svelto; Giovanna Valenti This study provides the first evidence that actin reorganization during AQP2 vesicular trafficking to the plasma membrane requires the functional involvement of ERM (ezrin/radixin/moesin...

... NHERF Cell spreading Migration Merlin ERM The Na + /H + exchanger regulatory factor (NHERF), also known as EBP50 (ERM-binding phosphoprotein 50) is a multifunctional adapter protein that possesses two related PDZ (PSD-95/Dlg/ZO-1) domains. Although initially isolated as an essential...

... migration. J. Biol. Chem. 276 , 7327 -7336. Bretscher, A., Edwards, K. and Fehon, R. G. ( 2002 ). ERM proteins and merlin: integrators at the cell cortex. Nat. Rev. Mol. Cell. Biol. 3 , 586 -599. Brown, M. S., Ye, J., Rawson, R. B. and Goldstein, J. L. ( 2000 ). Regulated intramembrane...

...Ken Hayashi; Shigenobu Yonemura; Takeshi Matsui; Sachiko Tsukita; Shoichiro Tsukita ABSTRACT Ezrin/radixin/moesin (ERM) proteins are thought to play an important role in organizing cortical actin-based cytoskeletons through cross-linkage of actin filaments with integral membrane proteins. Recent...