...Alejandra Tomas; Sylwia Jones; Simon O. Vaughan; Daniel Hochhauser; Clare E. Futter ABSTRACT EGF receptor (EGFR) endocytosis is induced by stress in a manner dependent on the p38 MAPK family. Ligand and stresses such as X-rays, reportedly promote nuclear trafficking of endocytosed EGFR...

... interactions between the transmembrane domains of the metalloproteinase ADAM17 and its partner molecule Rhbdf2 as being crucial for the regulation of ADAM17-dependent EGF-receptor and TNFα signaling. Rhbdf2 iRhom2 ADAM17 TACE EGF receptor Previous studies had shown that the Rhbdf2 sinecure...

...Qian Wang; Xinmei Chen; Zhixiang Wang ABSTRACT We have shown previously that epidermal growth factor (EGF) receptor (EGFR) endocytosis is controlled by EGFR dimerization. However, it is not clear how the dimerization drives receptor internalization. We propose that EGFR endocytosis is driven...

...Arola Fortian; Alexander Sorkin ABSTRACT Activation of epidermal growth factor (EGF) receptor (EGFR) leads to its interaction with Grb2, a dual-function adapter mediating both signaling through Ras and receptor endocytosis. We used time-lapse three-dimensional imaging by spinning disk confocal...

... an Arf6-dependent recycling pathway for β1-integrin and EGF receptors, resulting in inhibition of cell migration and attenuation of signaling downstream of these receptors. Importantly, the phenotypes of decreased cell adhesion and increased cell migration observed following Rab35 knock down...

... transporter depends on ubiquitylation of lysines located in a C-terminal cluster. Glia 56 , 963 -974. Grovdal, L. M., Stang, E., Sorkin, A. and Madshus, I. H. ( 2004 ). Direct interaction of Cbl with pTyr 1045 of the EGF receptor (EGFR) is required to sort the EGFR to lysosomes for degradation. Exp...

... endocytosis of epidermal growth factor receptor by RNA interference. J. Biol. Chem. 279 , 16657 -16661. Johannessen, L. E., Pedersen, N. M., Pedersen, K. W., Madshus, I. H. and Stang, E. ( 2006 ). Activation of the epidermal growth factor (EGF) receptor induces formation of EGF receptor- and Grb2...

..., Tiam1 and Vav2 have been shown to transduce the growth signal from the EGF receptor to Rac1. To clarify the role of each GEF, we time-lapse imaged the EGF-induced activity change of Rac1 in A431 cells transfected with siRNA targeting each Rac1 GEF. Because knockdown of these GEFs suppressed EGF-induced...

...Amy Tsacoumango; Song Jae Kil; Liping Ma; Frank D. Sönnichsen; Cathleen Carlin One of the main goals of this study was to understand the relationship between an epidermal growth factor (EGF) receptor dileucine (LL)-motif (679-LL) required for lysosomal sorting and the protein ubiquitin ligase CBL...

... was not essential for Arp2/3-complex-mediated actin assembly at these sites or for EGF receptor-mediated endocytosis, N-WASP deficiency caused a marked reduction of EGF internalization. We conclude that the assembly of WASP subfamily proteins and associated factors at sites of clathrin-coated pit invagination...

...Annemieke A. de Melker; Gerda van der Horst; Jannie Borst c-Cbl associates with the activated EGF receptor before endocytosis. We here reveal that the capacity of c-Cbl to promote receptor internalization depends on its ubiquitin ligase activity, which functionally connects the EGF receptor...

.... * Author for correspondence (e-mail: m.p.oksvold@labmed.uio.no ) 1 11 2001 © The Company of Biologists Limited 2002 2002 UV EGF receptor Receptor internalisation Intracellular trafficking The effects of short wavelength UV radiation on signal transduction have been...

... living cells expressing autophosphorylation-competent EGF receptors. Neither probe colocalized with transferrin in the absence of EGF, demonstrating that the reduced level of accessible PtdIns(4,5) P 2 in endosomes is constitutive. Finally, a PtdIns(3,4,5) P 3 -specific probe, which monitors...

..., A. and Stahl, P. D. ( 2000 ). Epidermal growth factor and membrane trafficking: EGF receptor activation of endocytosis requires rab5a. J. Cell Biol . 151 , 539 -550. Bonifacino, J. S. and Weissman, A. ( 1998 ). Ubiquitin and the control of protein fate in the secretory and endocytic pathways. Annu...

... temperature and a dynamin mutant, we find that c-Cbl associates with and ubiquitinates the activated epidermal growth factor (EGF) receptor at the plasma membrane in the absence of endocytosis. With the aid of confocal microscopy and immunogold electron microscopy, we could demonstrate that c-Cbl associates...