...Alejandra Tomas; Sylwia Jones; Simon O. Vaughan; Daniel Hochhauser; Clare E. Futter ABSTRACT EGF receptor (EGFR) endocytosis is induced by stress in a manner dependent on the p38 MAPK family. Ligand and stresses such as X-rays, reportedly promote nuclear trafficking of endocytosed EGFR...

... interactions between the transmembrane domains of the metalloproteinase ADAM17 and its partner molecule Rhbdf2 as being crucial for the regulation of ADAM17-dependent EGF-receptor and TNFα signaling. Rhbdf2 iRhom2 ADAM17 TACE EGF receptor National Institutes of Health 10.13039/100000002...

...Qian Wang; Xinmei Chen; Zhixiang Wang ABSTRACT We have shown previously that epidermal growth factor (EGF) receptor (EGFR) endocytosis is controlled by EGFR dimerization. However, it is not clear how the dimerization drives receptor internalization. We propose that EGFR endocytosis is driven...

...Arola Fortian; Alexander Sorkin ABSTRACT Activation of epidermal growth factor (EGF) receptor (EGFR) leads to its interaction with Grb2, a dual-function adapter mediating both signaling through Ras and receptor endocytosis. We used time-lapse three-dimensional imaging by spinning disk confocal...

... an Arf6-dependent recycling pathway for β1-integrin and EGF receptors, resulting in inhibition of cell migration and attenuation of signaling downstream of these receptors. Importantly, the phenotypes of decreased cell adhesion and increased cell migration observed following Rab35 knock down...

... Limited 2009 2009 EGF receptor Clathrin Endocytosis Ubiquitin The epidermal growth factor receptor (EGFR; also known as ErbB1) is so far the best characterized of the four ErbB-family members (EGFR, ErbB2, ErbB3 and ErbB4). EGFR binds to EGF with high affinity in a specific and saturable...

... 2009 EGF receptor Total internal reflection fluorescence microscopy Endocytosis The epidermal growth factor receptor (EGFR) is a frequently studied canonical member of the receptor tyrosine kinase superfamily ( Atalay et al., 2003 ). EGFR family members play roles in important...

..., Tiam1 and Vav2 have been shown to transduce the growth signal from the EGF receptor to Rac1. To clarify the role of each GEF, we time-lapse imaged the EGF-induced activity change of Rac1 in A431 cells transfected with siRNA targeting each Rac1 GEF. Because knockdown of these GEFs suppressed EGF-induced...

...Amy Tsacoumango; Song Jae Kil; Liping Ma; Frank D. Sönnichsen; Cathleen Carlin One of the main goals of this study was to understand the relationship between an epidermal growth factor (EGF) receptor dileucine (LL)-motif (679-LL) required for lysosomal sorting and the protein ubiquitin ligase CBL...

... was not essential for Arp2/3-complex-mediated actin assembly at these sites or for EGF receptor-mediated endocytosis, N-WASP deficiency caused a marked reduction of EGF internalization. We conclude that the assembly of WASP subfamily proteins and associated factors at sites of clathrin-coated pit invagination...

...Annemieke A. de Melker; Gerda van der Horst; Jannie Borst c-Cbl associates with the activated EGF receptor before endocytosis. We here reveal that the capacity of c-Cbl to promote receptor internalization depends on its ubiquitin ligase activity, which functionally connects the EGF receptor...

... EGF receptor Receptor internalisation Intracellular trafficking It has previously been reported that EGFR kinase activity is required for ligand-induced sequestration of receptors into coated pits( Lamaze and Schmid, 1995 ) and for EGFR internalisation ( Wiley et al.,1991 ). In order to test...

... living cells expressing autophosphorylation-competent EGF receptors. Neither probe colocalized with transferrin in the absence of EGF, demonstrating that the reduced level of accessible PtdIns(4,5) P 2 in endosomes is constitutive. Finally, a PtdIns(3,4,5) P 3 -specific probe, which monitors...

... Endocytosis Tyrosine kinase receptor Rab5 Hrs Hgs EGF receptor Cbl The discovery that activated EGF receptor (EGFR) is internalised by endocytosis ( Haigler et al., 1979 ) led to speculation that this pathway provides a means for delivery of the ligand (EGF) to the nucleus, where it could...

... temperature and a dynamin mutant, we find that c-Cbl associates with and ubiquitinates the activated epidermal growth factor (EGF) receptor at the plasma membrane in the absence of endocytosis. With the aid of confocal microscopy and immunogold electron microscopy, we could demonstrate that c-Cbl associates...

...Péter Nagy; Attila Jenei; Achim K. Kirsch; János Szöllősi; Sándor Damjanovich; Thomas M. Jovin ABSTRACT ErbB2 (HER2, Neu), a member of the epidermal growth factor (EGF) receptor tyrosine kinase family, is often overexpressed in breast cancer and other malignancies. ErbB2 homodimerizes but also...

... 1999 © 1999 by Company of Biologists 1999 EGF receptor Clathrin Adaptor Clathrin coats function at the plasma membrane to promote rapid endocytosis of various receptors and other membrane proteins as well as soluble macromolecules and viruses (reviewed in Schmid, 1997...

... of focal adhesions in fibroblasts. We find that EGF treatment of NR6 fibroblasts presenting full-length WT EGF receptors (EGFR) reduces the fraction of cells presenting focal adhesions from ~60% to ~30% within 10 minutes. The dose dependency of focal adhesion disassembly mirrors that for EGF-enhanced cell...

... in the presence of mAb 425 05 07 1991 10 09 1991 © 1997 by Company of Biologists 1997 EGF receptor Programmed cell death Keratinocyte Integrin Fig. 1. Morphology of keratinocytes and other normal human cell types pretreated with EGF-R...

... salivary gland (SSG) served as model to assess the role of epidermal growth factor (EGF) receptor signaling during emergence of cell lineage diversity. Temporal fluctuations in EGF receptor mRNA levels coincident with crucial differentiative cell lineage transitions were revealed by RNase protection...