.... Published by The Company of Biologists Ltd 2023 Summary: Knockout of ErbB genes reveals that the ErbB1 and the ErbB2–ErbB3 complex mediate G1/S progression, and a ligand-independent ErbB2 activity is sufficient for preventing apoptosis at high cell density. ErbB EGF Cell proliferation...

... localization compared with other markers of in vivo ERK activation. EGF EGFR LET-23 LET-60 LIN-45 MEK-2 MAPK C. elegans Nuclear translocation nKTR KTR Vulval development Cell fate patterning National Institutes of Health http://dx.doi.org/10.13039/100000002 R01GM121625...

... line was revalidated by assessing the expression of epithelial membrane markers (E-cadherin and β-catenin), and responsiveness to EGF. A431 cells were grown in Dulbecco's modified Eagle Medium (DMEM) containing (MultiCell, Wisent) and 10% heat-inactivated fetal calf serum, at 37°C under 5% CO 2...

... (MVBs) for degradation. The regulatory mechanism underlying formation of intralumenal vesicles en route to generation of MVBs is not fully understood. In this study, we found that SH3YL1, a phosphoinositide-binding protein, had a vesicular localization pattern overlapping with internalized EGF...

... differentiate. Activation of epidermal growth factor (EGF) signaling mediated the proliferative response to matrix stiffness and depended on focal adhesion assembly and cytoskeletal tension. Comparison of normal skin with keloid scar tissue further revealed an upregulation of EGF signaling within the epidermis...

... role in sorting of the degradative cargoes. CIM6PR EGF SNX12 Endosomal maturation Intraluminal vesicles Retromer Endocytosis begins at the plasma membrane where distinct signaling events select a range of different cargoes for internalization ( Sigismund et al., 2012 ). Several...

... oxidation of a candidate upstream target, the inhibitor of κB kinase α (IKKα; encoded by CHUK ), as a mechanism of action. We show that IKKα is sulfenylated at a conserved cysteine residue in the kinase domain, which correlates with de-repression of EGF promoter activity and increased EGF expression. Thus...

... and EGF signaling during three-dimensional culture. Wnt3a and EGF induced the expression of the P2Y 2 receptor (P2Y 2 R, also known as P2RY2), and knockdown of P2Y 2 R suppressed tubular formation. A P2Y 2 R mutant that lacks nucleotide responsiveness rescued the phenotypes resulting from P2Y 2 R...

... acetylation sites with arginine residues prevents cytoplasmic accumulation of Net1A caused by deacetylase inhibition or EGF stimulation. By contrast, replacement of these sites with glutamine residues is sufficient for Net1A relocalization, RhoA activation and downstream signaling. Moreover, the N-terminal...

... directly. We show here that NGF- and EGF-induced initial cell extensions result in APC phosphorylation by the MAPK/ERK pathway, which, in parallel with inhibition of GSK-3β, promotes localization of APC to the tip of cell extensions. Whereas GSK-3β inhibition promotes APC binding and stabilization...

... signalling networks remain largely uncharacterized. Here we identify a signalling module composed of a fibronectin–α5β1-integrin–integrin-linked-kinase (ILK) complex that, in concert with epidermal growth factor (EGF) cues, cooperatively controls the formation of transient actin-based circular dorsal ruffles...

... by either epidermal growth factor (EGF) or NMDA. These findings support the idea that GSH might represent the most important buffer of NO toxicity in neuronal cells, and indicate that the disruption of cellular redox buffering controlled by GSH makes neuronal cells susceptible to endogenous physiological...

.... , Bailly M. , Wyckoff J. B. , Segall J. E. , Condeelis J. S. ( 1998 ). EGF stimulates an increase in actin nucleation and filament number at the leading edge of the lamellipod in mammary adenocarcinoma cells . J. Cell Sci. 111 , 199 - 211 . Chan A. Y. , Bailly M...

... keratinocytes was blocked using siRNA targeting Smads. Unexpectedly, we found that EGF blocked TGFβ1-mediated N-cadherin expression in oral keratinocytes and not in OSCC cells. Mechanistically, EGF enhanced Smad phosphorylation in the linker region, and attenuated TGFβ1-mediated phosphorylation of Smad at the C...

... for epidermal growth factor (EGF)-stimulated protrusion in MTLn3 carcinoma cells. When stimulated with EGF, carcinoma cells showed a rapid increase in activated Cdc42 that is primarily localized to the protruding edge of the cells. siRNA-mediated knockdown of Cdc42 expression caused a decrease in EGF-stimulated...

... is internalized from the cell surface with degradation or recycling as potential fates. However, the cellular machinery involved in cadherin internalization and recycling remains controversial. Here we investigated EGF-induced E-cadherin internalization. EGF stimulation of MCF-7 cells resulted in Rac1-modulated...

... PLP-189 of BPGAP1, with prolines 184 and 186 being indispensable for this interaction. Overexpression of EEN or BPGAP1 alone induced EGF-stimulated receptor endocytosis and ERK1/2 phosphorylation. These processes were further enhanced when EEN was present together with the wildtype...

... growth factor (EGF) receptor; however, the site of action of RhoB within the endocytic pathway is still unknown. RhoB exists as two prenylated forms in cells: geranylgeranylated RhoB (RhoB-GG) and farnesylated RhoB (RhoB-F). Here we use farnesyltransferase inhibitors (FTIs) to show that prenylation...

... (e-mail: [email protected] ) 25 7 2003 © The Company of Biologists Limited 2003 2003 Rab5 Epidermal Growth Factor EGF Endocytosis Various macromolecules and viruses enter cells by means of receptor-mediated endocytosis. This process is accomplished in small...

... in cultured keratinocytes exposed to epidermal growth factor (EGF), the signaling pathway that regulates the formation of these structures is not defined. In cultured human keratinocytes seeded on fibronectin, we found that protein-serine/threonine kinase inhibitors including staurosporine, induced...