... conserved mechanism to ensure that mitosis occurs in a timely manner. We identified many RNA transcripts that encode proteins involved in mitosis localized at the mitotic spindles in dividing sea urchin embryos and mammalian cells. Disruption of microtubule polymerization, kinesin-1 or dynein results...

...Reto Gassmann ABSTRACT The microtubule minus-end-directed motility of cytoplasmic dynein 1 (dynein), arguably the most complex and versatile cytoskeletal motor, is harnessed for diverse functions, such as long-range organelle transport in neuronal axons and spindle assembly in dividing cells...

... that the behaviour of motors in cells and in vitro behaviour is not always comparable. For instance, activated dynein and kinesin motors walk processivity over several tens of micrometres on MTs in in vitro assays ( McKenney et al., 2014 ; Schlager et al., 2014 ), but neither motor has been documented to do so...

... endosomal choreography, which includes bidirectional and stop-and-go motions, is coordinated by the microtubule-based motor proteins dynein and kinesin. These motors bridge various endosomal subtypes to the microtubule tracks thanks to their cargo-binding domain interacting with endosome-associated proteins...

... called the fibrous corona as microtubule attachments form. This shedding is mediated, in part, by the minus-end directed motor dynein, which ‘strips’ cargoes along K-fibre microtubules. Despite its essentiality, little is known about how dynein stripping is regulated and how it responds to attachment...

... following streaming-dependent delivery. Actin reorganization, the actin-based motor myosin-V, RNA-binding proteins (such as Osk, Rump and Lost), cortical microtubules and microtubule-based motors (kinesin-1 and dynein) are all implicated in capturing and accumulating specific mRNAs and proteins...

... on a JEOL JEM1200 microscope equipped with a TVIPS F 416 camera at a nominal magnification of 41,000×, resulting in a pixel size of 0.38 nm. The dynein motor domain was purified and biotinylated as described previously ( Baclayon et al., 2017 ; Reck-Peterson et al., 2006 ). EB3 and the MACF2 C...

...Kari H. Ecklund; Megan E. Bailey; Kelly A. Kossen; Carsten K. Dietvorst; Charles L. Asbury; Steven M. Markus ABSTRACT Dynein motors move the mitotic spindle to the cell division plane in many cell types, including in budding yeast, in which dynein is assisted by numerous factors including...

... demonstrated to be dependent on the proteasome. We show that BBS1 regulates this process by coupling the 19S proteasome regulatory subunit to the microtubule motor dynein for its transport to the centrosome. Our data identify the ciliopathy-related protein BBS1 as a new player in T cell synapse assembly...

...Stephen M. King ABSTRACT Axonemal dyneins power the beating of motile cilia and flagella. These massive multimeric motor complexes are assembled in the cytoplasm, and subsequently trafficked to cilia and incorporated into the axonemal superstructure. Numerous cytoplasmic factors are required...

... in building the cilium by shuttling structural components and signaling receptors between the ciliary base and tip. To provide effective transport, IFT-A and IFT-B adaptor protein complexes assemble into highly repetitive polymers, called IFT trains, that are powered by the motors kinesin-2 and IFT-dynein...

... with mitochondria – the actin-based myosins, as well as the microtubule-based kinesins and dynein. In addition, Miro and TRAK proteins act as adaptors that link kinesin-1 and dynein, as well as myosin of class XIX (MYO19), to mitochondria and coordinate microtubule- and actin-based motor activities. Here, we...

...Gina A. Monzon; Lara Scharrel; Ashwin DSouza; Verena Henrichs; Ludger Santen; Stefan Diez ABSTRACT The maintenance of intracellular processes, like organelle transport and cell division, depend on bidirectional movement along microtubules. These processes typically require kinesin and dynein motor...

...Safia Omer; Katia Brock; John Beckford; Wei-Lih Lee ABSTRACT The current model for spindle positioning requires attachment of the microtubule (MT) motor cytoplasmic dynein to the cell cortex, where it generates pulling force on astral MTs to effect spindle displacement. How dynein is anchored...

... structure of the Chlamydomonas flagella radial spoke was obtained using single particle cryo-EM. Combined with cross-linking mass spectrometry and biophysics data, this allowed localization of components in the radial spoke head and neck regions. Cilia Cryo-electron microscopy Dynein Flagella...

..., we test the model in RPE cells by imaging melanosomes associated with labeled actin filaments and microtubules, and by investigating the roles of different motor proteins. We found that a deficiency in cytoplasmic dynein phenocopies the lack of myosin-7a, in that melanosomes undergo fewer of the slow...

...Riya Keshri; Ashwathi Rajeevan; Sachin Kotak ABSTRACT Proper orientation of the mitotic spindle is critical for accurate development and morphogenesis. In human cells, spindle orientation is regulated by the evolutionarily conserved protein NuMA, which interacts with dynein and enriches...

... at the immunological synapse. Furthermore, DISC1, Girdin and dynein are bound in a complex. Although this complex initially forms as a central patch at the synapse, it relocates to a peripheral ring corresponding to the peripheral supramolecular activation cluster (pSMAC). In the absence of DISC1, the classic actin...

...Amit Sharma; Sunayana Dagar; Sivaram V. S. Mylavarapu ABSTRACT The molecular motor dynein is essential for mitotic spindle orientation, which defines the axis of cell division. The light intermediate chain subunits, LIC1 and LIC2, define biochemically and functionally distinct vertebrate dynein...

...Kristi McElmurry; Jessica E. Stone; Donghan Ma; Phillip Lamoureux; Yueyun Zhang; Michelle Steidemann; Lucas Fix; Fang Huang; Kyle E. Miller; Daniel M. Suter ABSTRACT Previously, we have shown that bulk microtubule (MT) movement correlates with neurite elongation, and blocking either dynein activity...