... away from a stimulus) remains poorly understood. Proliferating Dictyostelium discoideum cells secrete a chemorepellent protein called AprA. Examining existing knockout strains, we previously identified proteins required for AprA-induced chemorepulsion, and a genetic screen suggested that the enzyme...

...Cristina Bosmani; Florence Leuba; Nabil Hanna; Frauke Bach; Frédéric Burdet; Marco Pagni; Monica Hagedorn; Thierry Soldati ABSTRACT Flotillins are lipid raft residents involved in membrane trafficking and recycling of plasma membrane proteins. Dictyostelium discoideum uses phagocytosis to kill...

... and uptake of mycobacteria, respectively, and generally in host cell defences against intracellular pathogens. Here, we show that the Dictyostelium discoideum LIMP-2 homologue LmpA regulates phagocytosis and phagolysosome biogenesis. The lmpA knockdown mutant is highly affected in actin-dependent processes...

...Hironori Inaba; Koji Yoda; Hiroyuki Adachi ABSTRACT Macropinocytosis involves the uptake of large volumes of fluid, which is regulated by various small GTPases. The Dictyostelium discoideum protein GflB is a guanine nucleotide exchange factor (GEF) of Rap1, and is involved in chemotaxis. Here, we...

...Joern Linkner; Gregor Witte; Hongxia Zhao; Alexander Junemann; Benjamin Nordholz; Petra Runge-Wollmann; Pekka Lappalainen; Jan Faix ABSTRACT Here, we analyzed the single inverse Bin/Amphiphysin/Rvs (I-BAR) family member IBARa from Dictyostelium discoideum . The X-ray structure of the N-terminal I...

...Barbara Peracino; Simona Buracco; Salvatore Bozzaro Summary The Dictyostelium discoideum genome harbors two genes encoding members of the Nramp superfamily, which is conserved from bacteria (MntH proteins) to humans (Slc11 proteins). Nramps are proton-driven metal ion transporters with a preference...

...Colin P. McCann; Paul W. Kriebel; Carole A. Parent; Wolfgang Losert Collective migration is a key feature of the social amoebae Dictyostelium discoideum , where the binding of chemoattractants leads to the production and secretion of additional chemoattractant and the relay of the signal...

... ) 13 8 2009 © The Company of Biologists Limited 2009 2009 Chemotaxis cAMP FRET Dictyostelium discoideum The ubiquitous second messenger cAMP regulates a wide range of cellular processes, including differentiation, proliferation and cell motility. Cyclic AMP is also essential...

... ; Overstreet et al., 2003 ; Traub, 2003 ; Wang and Struhl, 2005 ). Dissecting how epsin and other adaptors function in eukaryotic cells to tailor clathrin-coated pits amidst large volumes of membrane traffic remains an important challenge. Dictyostelium discoideum strains included Ax2, an axenic...

..., we show by single-molecule microscopy an agonist-induced increase in the mobile fraction of cAMP-receptor at the leading edge of chemotacting Dictyostelium discoideum cells. The onset of receptor mobility was correlated to the uncoupling and activation of the Gα2-protein. A finite-element simulation...

...Steve J. Charette; Pierre Cosson Chediak-Higashi syndrome (CHS) is characterized at the cellular level by a defect in the ability of cells to secrete lysosomes. However, the precise step affected in the secretion process is unclear. We characterized Dictyostelium discoideum cells containing...

... for correspondence (e-mail: [email protected] ) 28 2 2007 © The Company of Biologists Limited 2007 2007 Traction forces Myosin II Myosin essential light chain Movement Dictyostelium discoideum It is generally accepted that cell movement requires the production of myosin-II...

... to the phagosome, but little is known about membrane sorting during the early stages of phagosome formation. Here we used Dictyostelium discoideum amoebae to analyze the membrane composition of newly formed phagosomes. The membrane delimiting the closing phagocytic cup was essentially derived from the plasma...

.... 33 -46. Basel: Birkhauser. Fache, S., Dalous, J., Engelund, M., Hansen, C., Chamaraux, F., Fourcade, B., Satre, M., Devreotes, P. and Bruckert, F. ( 2005 ). Calcium mobilization stimulates Dictyostelium discoideum shear-flow-induced cell motility. J. Cell Sci. 118 , 3445 -3457. Fedier...

...Valentina Mercanti; Cédric Blanc; Yaya Lefkir; Pierre Cosson; François Letourneur The mechanisms responsible for the targeting of transmembrane integral proteins to the contractile vacuole (CV) network in Dictyostelium discoideum are unknown. Here we show that the transfer of the cytoplasmic...

... reorganization. Here we describe the cellular localization of myosin-IE and its role in the phagocytic uptake of solid particles and cells. A complete analysis of the kinetic and motor properties of Dictyostelium discoideum myosin-IE was achieved by the use of motor domain constructs with artificial lever arms...

...Sébastien Fache; Jérémie Dalous; Mads Engelund; Christian Hansen; François Chamaraux; Bertrand Fourcade; Michel Satre; Peter Devreotes; Franz Bruckert Application of hydrodynamic mild shear stress to adherent Dictyostelium discoideum vegetative cells triggers active actin cytoskeleton...

... by the chemotactic signal. * Author for correspondence (e-mail: [email protected] ) 21 2 2005 © The Company of Biologists Limited 2005 2005 3D-DIAS reconstruction Cell migration Filopodia Myosin II heavy chain phosphorylation Dictyostelium discoideum Animal cells...

... difficult to observe in mammalian cells. Taking advantage of the simplicity of the endosomal structure in Dictyostelium discoideum , we could visualize intraendosomal budding at the ultrastructural level. In this model organism, the drug U18666A was shown to stimulate intra-endosomal budding, while...

... for correspondence (e-mail: [email protected] ) 26 6 2003 © The Company of Biologists Limited 2003 2003 Motility Hydrodynamic flow Mechanosensitivity Dictyostelium discoideum In addition to specialized cell types such as hair cells, many eukaryotic cells exhibit force sensitivity...