... phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P 3 ] to phosphatidylinositol 4,5-bisphosphate [PI(4,5)P 2 ]. Dictyostelium discoideum cells require both PTEN and CnrN to induce chemorepulsion of cells away from the secreted chemorepellent protein AprA. How D. discoideum cells utilize two proteins with redundant...

... light on both the regulation and function of atypical MAPKs in different organisms. In particular, the analysis of the atypical MAPK in the amoeba Dictyostelium discoideum has revealed important roles in chemotactic responses and gene regulation. The rapid and transient phosphorylation of the atypical...

...Shigehiko Yumura; Masaki Nakano; Aika Honda; Yuuki Hashimoto; Tomo Kondo ABSTRACT Cyclic guanosine 3′,5′-monophosphate (cGMP) is a ubiquitous important second messenger involved in various physiological functions. Here, intracellular cGMP (cGMPi) was visualized in chemotactic Dictyostelium cells...

...Ramesh Rijal; Sara A. Kirolos; Ryan J. Rahman; Richard H. Gomer ABSTRACT Dictyostelium discoideum is a unicellular eukaryote that eats bacteria, and eventually outgrows the bacteria. D. discoideum cells accumulate extracellular polyphosphate (polyP), and the polyP concentration increases...

...Jeffrey A. Hadwiger; Huaqing Cai; Ramee G. Aranda; Saher Fatima ABSTRACT The Dictyostelium atypical mitogen-activated protein kinase (MAPK) Erk2 is required for chemotactic responses to cAMP as amoeba undergo multicellular development. In this study, Erk2 was found to be essential for the cAMP...

...Günther Gerisch; Jana Prassler; Mary Ecke ABSTRACT In multi-nucleate cells of Dictyostelium , cytokinesis is performed by unilateral cleavage furrows that ingress the large cells from their border. We use a septase (sepA)-null mutant with delayed cytokinesis to show that in anaphase a pattern...

...Yiwei Zhang; Hui Tu; Yazhou Hao; Dong Li; Yihong Yang; Ye Yuan; Zhonglong Guo; Lei Li; Haibin Wang; Huaqing Cai ABSTRACT Macropinocytosis mediates non-selective bulk uptake of extracellular fluid. It is the major route by which axenic Dictyostelium cells obtain nutrients and has emerged...

...Shigenori Hirose; Mariko Katoh-Kurasawa; Gad Shaulsky ABSTRACT Allorecognition and tissue formation are interconnected processes that require signaling between matching pairs of the polymorphic transmembrane proteins TgrB1 and TgrC1 in Dictyostelium . Extracellular and intracellular cAMP signaling...

... distinct, but conserved, glycine residues in both the Dictyostelium piaA gene and its human ortholog, RICTOR . The two conserved residues are spaced ∼50 amino acids apart, and both are embedded within a conserved region falling in between the Ras-GEFN2 and Rictor­_V domains. The effects of point mutations...

...Thomas D. Williams; Sew-Yeu Peak-Chew; Peggy Paschke; Robert R. Kay ABSTRACT Macropinocytosis is an actin-driven process of large-scale and non-specific fluid uptake used for feeding by some cancer cells and the macropinocytosis model organism Dictyostelium discoideum . In Dictyostelium...

... of reactive oxygen species, deprivation of nutrients and the lumenal accumulation of toxic metals are essential to kill ingested bacteria or inhibit the growth of intracellular pathogens. Here, we used the soil amoeba Dictyostelium discoideum , a professional phagocyte that digests bacteria for nutritional...

...Lauriane Mas; Adeline Cieren; Christian Delphin; Agnès Journet; Laurence Aubry ABSTRACT Arrestins are key adaptor proteins that control the fate of cell-surface membrane proteins and modulate downstream signaling cascades. The Dictyostelium discoideum genome encodes six arrestin-related proteins...

...Weiye Wang; Song Chen; Satarupa Das; Wolfgang Losert; Carole A. Parent ABSTRACT Dictyostelium discoideum cells transport adenylyl cyclase A (ACA)-containing vesicles to the back of polarized cells to relay exogenous cAMP signals during chemotaxis. Fluorescence in situ hybridization (FISH...

...-proteins and the cytoskeleton. Small G-proteins Cytoskeleton Symmetry Dictyostelium Chemotaxis Nederlandse Organisatie voor Wetenschappelijk Onderzoek 10.13039/501100005909 723.012.108 The nature of physics is symmetrical. For instance, molecules have equal probability...

...Thomas D. Williams; Robert R. Kay ABSTRACT Macropinocytosis is a conserved endocytic process used by Dictyostelium amoebae for feeding on liquid medium. To further Dictyostelium as a model for macropinocytosis, we developed a high-throughput flow cytometry assay to measure macropinocytosis...

...Shigenori Hirose; Gong Chen; Adam Kuspa; Gad Shaulsky ABSTRACT Allorecognition is a key factor in Dictyostelium development and sociality. It is mediated by two polymorphic transmembrane proteins, TgrB1 and TgrC1, which contain extracellular immunoglobulin domains. TgrB1 and TgrC1 are necessary...

...Patrick M. Suess; Jacob Watson; Wensheng Chen; Richard H. Gomer ABSTRACT Linear chains of five to hundreds of phosphates called polyphosphate are found in organisms ranging from bacteria to humans, but their function is poorly understood. In Dictyostelium discoideum , polyphosphate is used...

...Peter A. Thomason; Jason S. King; Robert H. Insall ABSTRACT The steps leading to constitutive exocytosis are poorly understood. In Dictyostelium WASH complex mutants, exocytosis is blocked, so cells that take up fluorescent dextran from the medium retain it and remain fluorescent. Here, we...

...Barbara Pergolizzi; Enrico Bracco; Salvatore Bozzaro ABSTRACT Cyclic AMP (cAMP) binding to G-protein-coupled receptors (GPCRs) orchestrates chemotaxis and development in Dictyostelium. By activating the RasC–TORC2–PKB (PKB is also known as AKT in mammals) module, cAMP regulates cell polarization...

.... Food is a common source of mutagens, but little is known about how nutritional factors cause lasting genetic changes in the consuming organism. Here, we describe an unusual genetic interaction between DNA repair in the unicellular amoeba Dictyostelium discoideum and its natural bacterial food source...