.... Phosphatidic acid (PA) and diacylglycerol (DG) can both serve as biosynthetic precursors for membrane lipid synthesis. How they are regulated in time and space and what the implications are of changes in their flux for mitotic fidelity are largely unknown. Using genetically encoded PA and DG probes, we show...

... homeostasis. ESCRT-, V-ATPase- and vacuole protein sorting-mutants negatively affect LD consumption, independent of lipophagy. Loss of ESCRT function leads to the accumulation of LD-derived diacylglycerol (DAG), preventing its conversion into phosphatidic acid (PA) and membrane lipids. In addition, channeling...

...Enric Gutiérrez-Martínez; Inés Fernández-Ulibarri; Francisco Lázaro-Diéguez; Ludger Johannes; Susan Pyne; Elisabet Sarri; Gustavo Egea Summary The inhibition of phosphatidic acid phosphatase (PAP) activity by propanolol indicates that diacylglycerol (DAG) is required for the formation of transport...

...Takuro Numaga; Motohiro Nishida; Shigeki Kiyonaka; Kenta Kato; Masahiro Katano; Emiko Mori; Tomohiro Kurosaki; Ryuji Inoue; Masaki Hikida; James W. Putney, Jr; Yasuo Mori Ca 2+ signaling mediated by phospholipase C that produces inositol 1,4,5-trisphosphate [Ins(1,4,5) P 3 ] and diacylglycerol (DAG...

..., H., Zizak, M., Divecha, N. and Irvine, R. F. ( 1993 ). Nuclear diacylglycerol is increased during cell proliferation in vivo. Biochem. J. 290 , 633 -636. Beaudouin, J., Gerlich, D., Daigle, N., Eils, R. and Ellenberg, J. ( 2002 ). Nuclear envelope breakdown proceeds by microtubule...

...+ release from internal stores, the majority of the requirement for PtdIns(4,5) P 2 hydrolysis occurs downstream of Ca 2+ signalling - as shown in permeabilised cells, where the inositol (1,4,5)-trisphosphate-Ca 2+ pathway is bypassed. Neither generation of the PtdIns(4,5) P 2 metabolite, diacylglycerol...

...-step process that is very precisely coordinated. Chimaerins are Rac1-specific GTPase-activating proteins of unknown biological function, which have a canonical diacylglycerol C1-binding domain. Here we demonstrate endogenous expression of β2-chimaerin in T lymphocytes and study the functional role...

... previously to translocate to the nucleus at G 2 /M and this was coupled to the generation of nuclear diacylglycerol. However, it is not clear how isoenzyme selective translocation and nuclear targeting is achieved during cell cycle. To investigate further the role of nuclear diacylglycerol we measured PKC...

...PAUL M. LARSEN; TUNG-LING L. CHEN; STEPHEN M. WOLNIAK Neomycin has been reported to inhibit polyphosphoinositide cycling by preventing the hydrolysis of phosphatidylinositol 4,5-bisphosphate into inositol 1,4,5-trisphosphate and 1,2-diacylglycerol. Inositol 1,4,5-trisphosphate, through...