... microtubule growth and shrinkage. Strikingly, APC-C accumulates at shrinking microtubule extremities, a common characteristic of depolymerases. Cryo-electron microscopy revealed that APC-C adopts an extended conformation along the protofilament crest and showed the presence of ring-like tubulin oligomers...

... containing the motor domain are functional MT depolymerases. To determine whether this activity was due to the catalytic ATPase activity of the motor, we performed an in vivo MT-depolymerisation assay ( Stumpff et al., 2007 ). This entailed generating a cell line expressing a motor-dead rigor form, Klp67A...