...Takuya Hayakawa; Asahi Yamamoto; Taiki Yoneda; Sakino Hori; Nanami Okochi; Kazuhiro Kagotani; Katsuzumi Okumura; Shin-ichiro Takebayashi ABSTRACT The temporal order of DNA replication along the chromosomes is thought to reflect the transcriptional competence of the genome. During differentiation...

...Caitlin Connolly; Saori Takahashi; Hisashi Miura; Ichiro Hiratani; Nick Gilbert; Anne D. Donaldson; Shin-Ichiro Hiraga ABSTRACT The organisation of chromatin is closely intertwined with biological activities of chromosome domains, including transcription and DNA replication status. Scaffold...

... replication stress. TDP-43 nucleic-acid-binding and self-assembly activities are important in inhibiting R-loop accumulation and preserving normal DNA replication. We also found that TDP-43 cytoplasmic aggregation impairs TDP-43 function in R-loop regulation. Furthermore, increased R-loop accumulation and DNA...

...Yi-Wen Xu; Lan-Rui Cao; Min Wang; Ying Xu; Xin Wu; Junping Liu; Chao Tong; Heng-Yu Fan ABSTRACT Precise regulation of DNA replication and genome integrity is crucial for gametogenesis and early embryogenesis. Cullin ring-finger ubiquitin ligase 4 (CRL4) has multiple functions in the maintenance...

...Eyemen Kheir; Torsten Krude ABSTRACT Non-coding Y RNAs are essential for the initiation of chromosomal DNA replication in vertebrates, yet their association with chromatin during the cell cycle is not characterised. Here, we quantify human Y RNA levels in soluble and chromatin-associated...

.... We demonstrate that sbRNAs from a range of nematode species are able to reconstitute the initiation of chromosomal DNA replication in the presence of replication proteins in vitro , and that conserved nucleotide sequence motifs are essential for this function. By functionally inactivating sbRNAs...

...Nikki A. Copeland; Heather E. Sercombe; Rosemary H. C. Wilson; Dawn Coverley CIZ1 is a nuclear matrix protein that cooperates with cyclin A2 (encoded by CCNA2 ) and CDK2 to promote mammalian DNA replication. We show here that cyclin‐A–CDK2 also negatively regulates CIZ1 activity by phosphorylation...

...Mona Yekezare; Belén Gómez-González; John F. X. Diffley Summary DNA replication in eukaryotic cells initiates from multiple replication origins that are distributed throughout the genome. Coordinating the usage of these origins is crucial to ensure complete and timely replication of the entire...

... transcription and replication imaging protocol), that reveals simultaneously mtDNA and RNA, and that can also be coupled to immunofluorescence for in situ protein examination. mTRIP reveals mitochondrial structures engaged in initiation of DNA replication by identification of a specific sequence...

... Ltd 2013 DNA replication Eco1/Ctf7 SMC3 Sister chromatid cohesion Acetylation Condensation One goal of cell division is to ensure that sister chromatids, produced during S phase, become properly segregated into the newly forming daughter cells during mitosis. To accomplish...

... of recombination. Following the initiation of DNA replication, the induction of these foci by replication stress is suppressed by defects in origin recognition complex (ORC) function, which is accompanied by loss of viability and severe mitotic defects. This suggests that cells lacking Brc1 undergo an ORC...

... is stabilised or activated, both of which can contribute to preventing licensing occurring at this time. We show that synchronised U2OS cells progressing through their first S phase in the absence of geminin exhibit no large-scale defect in DNA replication. This is probably due to the ability of PCNA-mediated...

... of replication in higher eukaryotes and a means to dissect Orc-dependent and Orc-independent events at origins. * Author for correspondence ( s.cotterill@sgul.ac.uk ) 5 9 2011 © 2012. 2012 Orc DNA replication Origin of replication Gal4 Drosophila S2 cells Histone acetylation...

...Madhav Jagannathan; Amos M. Sakwe; Tin Nguyen; Lori Frappier Mini-chromosome maintenance complex-binding protein (MCM-BP) was discovered as a protein that is strongly associated with human MCM proteins, known to be crucial for DNA replication in providing DNA helicase activity. The Xenopus MCM-BP...

...Rentian Wu; Jiafeng Wang; Chun Liang Regulation of DNA replication initiation is essential for the faithful inheritance of genetic information. Replication initiation is a multi-step process involving many factors including ORC, Cdt1p, Mcm2-7p and other proteins that bind to replication origins...

...Alice Tianbu Zhang; Alexander R. Langley; Christo P. Christov; Eyemen Kheir; Thomas Shafee; Timothy J. Gardiner; Torsten Krude Non-coding Y RNAs are required for the initiation of chromosomal DNA replication in mammalian cells. It is unknown how they perform this function or if they associate...

... in mitotic exit, providing new insight into the intricate regulation of DNA replication through the interplay of CDKs and the Cdc14p phosphatase. Cdc14p phosphatase DNA replication Replication licensing Replication-initiation proteins Cell cycle Budding yeast * Author for correspondence...

... College London, Department of Genetics, Evolution and Environment and UCL Cancer Institute, London, WC1E 6BT, UK 9 6 2010 © 2010. 2010 Cell cycle DNA amplification DNA replication Fission yeast Replication origin For stable genome inheritance, S phase occurs once per cell...

..., a large number of proteins involved in DNA repair and DNA replication are either recruited to or redistributed from nucleoli in response to genotoxic stress ( Dellaire and Bazett-Jones, 2007 ; Sacher et al., 2006 ; Torres-Rosell et al., 2007 ). How hCdc14B is targeted to the nucleolus remains unclear...

... in the initiation of DNA replication. Here, we present evidence that 14-3-3 proteins are novel regulators of the initiation and elongation steps of DNA replication in Saccharomyces cerevisiae . The results show that the Bmh2 protein, one of the two 14-3-3 homologues in S. cerevisiae , interacts with Mcm2 and Orc2...