... or Mann–Whitney's U test, one-way ANOVA with Dunnett's post-hoc, or two-way ANOVA with Sidak's post-hoc tests. All sequencing P -values are adjusted for multiple comparisons (Benjamini–Hochberg method, FDR). All analysis for the assessment of differential DNA methylation (bisulfite data...

... DNA methylation and histone modifications were significantly altered in the stem-like and non-stem phenotypes. In particular, in the stem-like phenotype, the miR-200b-200a-429 cluster was silenced primarily through polycomb group-mediated histone modifications whereas the miR-200c-141 cluster...

...Joe Burrage; Ausma Termanis; Andreas Geissner; Kevin Myant; Katrina Gordon; Irina Stancheva Summary LSH, a protein related to the SNF2 family of chromatin-remodelling ATPases, is essential for the correct establishment of DNA methylation levels and patterns in plants and mammalian cells. However...

... such as migration, extracellular matrix production and assembly in 3D self-assembled tissues. To determine potential regulatory regions predictive of expression of PDGFRβ following differentiation from ESCs and iPSCs, we analyzed the DNA methylation status of a region of the PDGFRB promoter that contains multiple...

... FERTILIZATION INDEPENDENT SEED genes DNA methylation Polycomb group proteins A subset of genes in mammals and flowering plants is only expressed from one of the two homologous chromosomes, depending on whether they are maternally or paternally inherited. This process is called genomic imprinting...

... hybridization, Aurora-B and H3S10 phosphorylated foci were found more frequently at larger pericentromeres than at smaller ones, revealing a preferential phosphorylation of pericentromeres, exhibiting a high density of methyl cytosines. Disruption of DNA methylation inhibited pericentromeric Aurora-B targeting...

... Limited 2006 2006 DNA methylation Transcriptional repression Epigenetics Methyl CpG binding protein Eukaryotic chromosomes are the repositories of the genetic information necessary to direct the synthesis of cellular components. Information is embedded in them at multiple levels...

... and induced changes in DNA methylation and histone acetylation, using in situ hybridisation and confocal 3D imaging. After germinating seeds either in the presence of 5-Azacytidine (5-AC), which leads to DNA hypomethylation, or trichostatin A (TSA), which results in histone hyperacetylation, the architecture...

...Adrian Bird; Peri Tate; Xinsheng Nan; Javier Campoy; Richard Meehan; Sally Cross; Susan Tweedie; Jillian Charlton; Donald Macleod SUMMARY We have been studying the evolution and function of DNA methylation in vertebrate animals using three related approaches. The first is to further characterise...

... these effects may be medi­ ated by proteins that bind to methylated DNA. Méthylation of DNA is essential in mammals In mammals approximately 70% of all CpGs are methy­ lated at the 5 position of cytosine. It is not clear what the functional role of this modification is, but it has been recently shown by gene...

...Stephen G. Grant; Ronald G. Worton ABSTRACT Treatment with 5-azacytidine, a potent inhibitor of DNA methylation, was used to induce activation of the selectable hprt gene on the inactive X chromosome in a diploid female Chinese hamster cell line. The transformed, stably diploid cell line F3B...