.../nucl.21055 Arnould , C. , Rocher , V. , Saur , F. , Bader , A. S. , Muzzopappa , F. , Collins , S. , Lesage , E. , Le Bozec , B. , Puget , N. , Clouaire , T. et al. ( 2023 ). Chromatin compartmentalization regulates the response to DNA damage...

... of the nuclear alterations also contain autophagic proteins and type II DNA topoisomerases (TOP2A and TOP2B), or the nucleolar protein fibrillarin, implying they are also targets of nucleophagy. We propose that basal nucleophagy contributes to genome and nuclear stability, as well as in response to DNA damage...

... constrained by concerns of unequal nuclear expansion. Here, we demonstrate the conditions for isotropic expansion of the nucleus at a resolution equal to or better than 120–130 nm (pre-expansion). Using the DNA damage response proteins BRCA1, 53BP1 (also known as TP53BP1) and RAD51 as exemplars, we...

...Gérald Lossaint; Anđela Horvat; Véronique Gire; Katarina Bačević; Karim Mrouj; Fabienne Charrier-Savournin; Virginie Georget; Daniel Fisher; Vjekoslav Dulić ABSTRACT Senescence is an irreversible withdrawal from cell proliferation that can be initiated after DNA damage-induced cell cycle arrest...

... mediators, such as Akt, ERK, p53 and p21. Knockdown of RPA abrogated IGF-I-mediated responses in keratinocytes, thereby establishing that post-UVBR IGF-I photoprotection required a functional RPA. Our investigation identifies RPA as the functional link between IGF-1R-mediated cellular effects, DNA damage...

...Matteo Cabrini; Marco Roncador; Alessandro Galbiati; Lina Cipolla; Antonio Maffia; Fabio Iannelli; Simone Sabbioneda; Fabrizio d’Adda di Fagagna; Sofia Francia ABSTRACT The DNA damage response (DDR) is the signaling cascade that recognizes DNA double-strand breaks (DSBs) and promotes...

... in processes such as ciliogenesis. Human CDC14 Mitosis Cytokinesis DNA damage Ciliogenesis CDC14 encodes an essential highly conserved dual specificity protein phosphatase that in budding yeast Saccharomyces cerevisiae promotes mitotic exit, the transition from mitosis into G1 phase...

..., promoting the epithelial–mesenchymal transition (EMT); however, the underlying mechanisms are unknown. We found that silica, matrix stiffening or their combination triggered Ac-α-Tub downregulation in alveolar epithelial cells, followed by DNA damage and replication stress. α-TAT1 elevated Ac-α-Tub to limit...

...Cedric Landmann; Priscillia Pierre-Elies; Damien Goutte-Gattat; Emilie Montembault; Marie-Charlotte Claverie; Anne Royou ABSTRACT The DNA damage sensor Mre11-Rad50-Nbs1 complex and Polo kinase are recruited to DNA lesions during mitosis. However, their mechanism of recruitment is elusive. Here...

... in structure of nuclear myosins, their nuclear import and their roles within transcription, DNA damage, chromatin organisation and viral infections. We will also consider both the biochemical and biophysical properties and restraints that are placed on these multifunctional motors, and how they link...

...Imke K. Mandemaker; Di Zhou; Serena T. Bruens; Dick H. Dekkers; Pernette J. Verschure; Raghu R. Edupuganti; Eran Meshorer; Jeroen A. A. Demmers; Jurgen A. Marteijn ABSTRACT Many chromatin remodeling and modifying proteins are involved in the DNA damage response, where they stimulate repair...

... that despite a similar DNA-damaging effect along the anteroposterior axis of intact animals, responses differed between anterior and posterior fragments after amputation. Stem cell proliferation and differentiation proceeded successfully in the amputated heads, leading to regeneration of missing tissues. Stem...

... depletion alters neither the apical localization of TnI or Bazooka, nor the basal localization of Dlg. Nevertheless, overexpressing PI3K prevents the defects seen upon TnI depletion. TnI loss-of-function disrupts cytoskeletal β-Catenin, E-Cadherin and γ-Tubulin, and causes an increase in DNA damage...

...Xiangduo Kong; Gladys Mae Saquilabon Cruz; Sally Loyal Trinh; Xu-Dong Zhu; Michael W. Berns; Kyoko Yokomori ABSTRACT TRF2 (TERF2) binds to telomeric repeats and is critical for telomere integrity. Evidence suggests that it also localizes to non-telomeric DNA damage sites. However, this recruitment...

...), culminating in the accumulation of DSBs. These effects were associated with a reduced expression of DNA damage signaling and repair proteins. Our data disclose a key role for dephosphorylation of PP1:NIPP1 substrates in setting the threshold for DNA repair, and indicate that activators of this phosphatase...

... divisions and that DNA damage promotes BR-dependent paracrine signaling in the stem cell niche as a prerequisite to stem cell replenishment. Surprisingly, when the plants that overexpressed BRI1 in the QC (pWOX5:BRI1-YFP) were assessed in terms of QC division rates, we found only a limited increase...

... of the trafficking defect induced by MNU. We have established a model for DNA damage-induced transformation and demonstrated that the transformation occurred via DNA-PK activation, but was independent of impaired cellular trafficking ( Fig. 8 D). Although this mechanism is responsible for polarity disruption...

... joining (NHEJ), which relies on 53BP1. The balance between NHEJ and HR depends, in part, on whether 53BP1 predominates in binding to damage sites, where it protects the DNA ends from resection. The nucleoporin Nup153 has been implicated in the DNA damage response, attributed to a role in promoting nuclear...

... that α-catenin is able to modulate the sensitivity of cells to DNA damage and toxicity. Furthermore, nuclear α-catenin is actively recruited to sites of DNA damage. This recruitment occurs in a β-catenin-dependent manner and requires nuclear actin polymerization. These findings provide mechanistic...

... tolerance to DNA damage. Here, we show that NIPBL is recruited to DNA damage throughout the cell cycle via independent mechanisms, influenced by type of damage. First, the heterochromatin protein HP1γ (also known as CBX3) recruits NIPBL to DNA double-strand breaks (DSBs) through the corresponding HP1...