... dysfunction. Chromatin and lamins are two of the major structural components of the nucleus that maintain its shape and function, but their relative roles in nuclear blebbing remain elusive. To determine the composition of nuclear blebs, we compared the immunofluorescence intensity of DNA and lamin B...

... methods. Although uncontested in their value for single-copy genes, mapping the chromatin of DNA repeats is problematic for biochemical techniques that involve averaging of cell populations or analysis of clusters of tandem repeats in a single-cell analysis. Extending chromatin and DNA fibers allows us...

...Ghislaine Pinon-Lataillade; Christel Masson; Jacqueline Bernardino-Sgherri; Véronique Henriot; Philippe Mauffrey; Yveline Frobert; Silvia Araneda; Jaime F. Angulo Genotoxic agents deform DNA structure thus eliciting a complex genetic response allowing recovery and cell survival. The Kin17 gene...

... been introduced to optimize the detection of DNA and RNA. The pervasiveness of this technique is largely because of its wide variety of applications and the relative ease of implementation and performance of in situ studies. Although the basic principles of FISH have remained unchanged, high...

...N. Gilbert; L. Lucas; C. Klein; M. Menager; N. Bonnet; D. Ploton ABSTRACT The relative three-dimensional co-location of RNA polymerase I (RPI) and DNA was studied using confocal laser scanning microscopy during interphase and all the steps of mitosis in human cancerous cells. For each step...

... mitogens growth factors immediate early genes delayed early genes cell cycle gene regulation cell metabolism DNA After exiting from mitosis, mammalian cells have the option to either start a new round of cell division or to leave the cell cycle and enter a resting state, usually referred...

...Kevin A. W. Lee ABSTRACT Dimeric transcription factors that bind to DNA are often grouped into families on the basis of dimerization and DNA-binding specificities. cDNA cloning studies have established that members of the same family have structurally related dimerisation and DNA-binding domains...

...E. Gwyn Jordan; David J. Rawlins ABSTRACT Spirogyra nucleoli were shown by three-dimensional optical microscopy of DAPI fluorescence to contain DNA with a pattern and distribution matching those of the fibrillar centres. This was confirmed using different species with nucleoli showing different...