... mechanisms responsible for the remodeling of constitutive heterochromatin are largely unknown. Here, we identify that DAXX, an H3.3 chaperone essential for the maintenance of mouse ESCs in the ground state, accumulates in pericentromeric regions independently of DNA methylation. DAXX recruits PML and SETDB1...

... enrichment of CENP-A in chromatin fractions. The depletion of DAXX, a histone H3.3 chaperone, suppressed CENP-A mislocalization and CIN in CHAF1B-depleted cells. We propose that in CHAF1B-depleted cells, DAXX promotes mislocalization of the overexpressed CENP-A to non-centromeric regions, resulting in CIN...

...Prashanth Krishna Shastrula; Isabel Sierra; Zhong Deng; Frederick Keeney; James E. Hayden; Paul M. Lieberman; Susan M. Janicki ABSTRACT The incorporation of the histone H3 variant, H3.3, into chromatin by the H3.3-specific chaperone DAXX and the ATP-dependent chromatin remodeling factor ATRX...

... the alternative lengthening of telomeres (ALT) mechanism. Mutations in the DAXX gene have been found frequently in both telomerase-positive and ALT cells, and how DAXX mutations contribute to cancers remains unclear. We report here that endogenous DAXX can localize to Cajal bodies, associate with the telomerase...

.... In this paper, we show that PIAS1 regulates ultraviolet (UV)-induced apoptosis by recruiting Death-associated protein 6 (Daxx) to PIAS1-generated SUMO-foci. Cells that ectopically express PIAS1, but not other PIASes, show increased sensitivity to UV irradiation, suggesting that PIAS1 has a distinct function...

...Alyshia Newhart; Ilona U. Rafalska-Metcalf; Tian Yang; Dmitri G. Negorev; Susan M. Janicki Summary Histone H3.3 is a constitutively expressed H3 variant implicated in the epigenetic inheritance of chromatin structures. Recently, the PML-nuclear body (PML-NB)/Nuclear Domain 10 (ND10) proteins, Daxx...

...Alexander M. Ishov; Olga V. Vladimirova; Gerd G. Maul Placing regulatory proteins into different multiprotein complexes should modify key cellular processes. Here, we show that the transcription repressor Daxx and the SWI/SNF protein ATRX are both associated with two intranuclear domains: ND10/PML...

... heat shock Daxx and Sp100 were released but PML remained, whereas exposure to subtoxic concentrations of CdCl 2 induced the release of ND10-associated proteins, including PML, with Sp100 remaining in a few sites. In both cases,recovery times were similar and were followed by a burst of mitotic activity...

...Jennifer S. Michaelson; Philip Leder The function of DAXX, a highly conserved mammalian gene, has remained controversial; this is due, in part, to its identification in a variety of yeast two-hybrid screens. Targeted deletion in the mouse revealed that DAXX is essential for embryonic development...

...Andrew D. Hollenbach; Craig J. McPherson; Edwin J. Mientjes; Rekha Iyengar; Gerard Grosveld Human Daxx is a protein that functions, in part, as a transcriptional co-repressor through its interaction with a growing number of nuclear,DNA-associated proteins. To determine the mechanism by which hDaxx...