...Aurélie Gueho; Cristina Bosmani; Navin Gopaldass; Virginie Molle; Thierry Soldati; François Letourneur ABSTRACT Proteins that contain Eps15 homology domains (EHDs) in their C-terminus are newly identified key regulators of endosomal membrane trafficking. Here, we show that D. discoideum contains...

... members regulate endocytic protein trafficking. Here, we explored the function of ACAP-A in the endocytic pathway of D. discoideum . In the absence of ACAP-A, the efficiency of fusion between post-lysosomes and the plasma membrane was reduced, resulting in the accumulation of post-lysosomes. Moreover...

... expressed in E. coli and bound to glutathione–Sepharose beads (GE Healthcare). For the interaction of ZizA and ZizB with Rac proteins, 3×10 8   D. discoideum cells expressing ZizB–GFP were lysed in a lysis buffer [25 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% Triton X-100, 1 mM NaF, 0.5 mM Na 3 VO...

... interaction Nramp1 PI3K PTEN PLC V-H + ATPase D. discoideum L. pneumophila E. coli Macrophages Phagocytosis is a property of ‘professional’ phagocytes capable of ingesting and killing a large variety of microorganisms. Professional phagocytes include specialized metazoan cells...

... for correspondence 27 05 1998 28 03 1998 © 1998 by Company of Biologists 1998 S. cerevisiae D. discoideum Mitochondrion Mitochondria supply most of the cellular ATP in eukaryotic cells. The accumulation of mitochondria near sites of high ATP consumption (i.e. near flagellar...

... 04 1990 © 1989 by Company of Biologists 1989 chemotaxis cyclic AMP folic acid D. discoideum G protein During the life cycle of the cellular slime mold Dictyostelium discoideum , two different extracellular signals play an important role. In the vegetative stage...

... reduced the affinity of the receptor at all time-points of the oscillation cycle to the minimal, i.e. adapted, level. We conclude that the cyclic process of activation, adaptation and de-adaptation in D. discoideum , at cyclic AMP receptor level, involves changes in receptor-G protein interaction. During...

... than 10 −7 M. In membranes derived from ras-Thrl2 cells addition of GTP or PMA had no effect. The results indicate that D. discoideum cells contain a cyclic AMP receptor-controlling pathway that can be activated in vitro and involves a GTP-binding protein and a Ca 2+ plus ATP-dependent activity...