... repressed state essential for cell survival in stressful environments. In the fission yeast Schizosaccharomyces pombe , cell size is reduced by cell division before entry into stationary phase; thus cyclin-dependent kinase (CDK) must actively contribute to stationary phase establishment. However...

...Souradeep Basu; James O. Patterson; Theresa U. Zeisner; Paul Nurse ABSTRACT The eukaryotic cell cycle is driven by the activity of cyclin-dependent kinases (CDKs). CDK activity rises over 50-fold during the cell cycle, from a low level in G1 to a high level in mitosis. However, it is not known...

...Yang Ma; Guanxiong Yan; Xiaojie Han; Jing Zhang; Jie Xiong; Wei Miao ABSTRACT To investigate the mechanisms underlying initiation of the sexual cell cycle in eukaryotes, we have focused on cyclins and cyclin-dependent kinases (CDKs) in the well-studied model ciliate, Tetrahymena thermophila . We...

... medium provided that the original work is properly attributed. Summary: Phosphorylation of importin-α1 by cyclin-dependent kinase 1 provides spatial and temporal control of mitotic spindle assembly through the release of spindle-assembly factors from inhibitory complexes during mitosis...

...Brendan L. Powers; Mark C. Hall ABSTRACT Inactivation of cyclin-dependent kinase (Cdk) and reversal of Cdk phosphorylation are universally required for mitotic exit. In budding yeast ( Saccharomyces cerevisiae ), Cdc14 is essential for both and thought to be the major Cdk-counteracting phosphatase...

...Daniel Fisher; Liliana Krasinska; Damien Coudreuse; Béla Novák Summary Fifteen years ago, it was proposed that the cell cycle in fission yeast can be driven by quantitative changes in the activity of a single protein kinase complex comprising a cyclin – namely cyclin B – and cyclin dependent kinase...

...Chang-Run Li; Jie-Ying Au Yong; Yan-Ming Wang; Yue Wang Cyclin-dependent kinases (CDKs) regulate septin organization in a cell-cycle-dependent manner in yeast. However, the mechanism remains unclear. Here, we show that the Candida albicans CDK Cdc28 phosphorylates the Nim1-related kinase Gin4...

...Dongli Hu; Marcus Valentine; Vincent J. Kidd; Jill M. Lahti Cyclin-dependent kinase 11 (CDK11) mRNA produces a 110-kDa protein (CDK11 p110 ) throughout the cell cycle and a 58-kDa protein (CDK11 p58 ) that is specifically translated from an internal ribosome entry site sequence during G2/M. CDK11...

...Stephen M. Huisman; Monique F. M. A. Smeets; Marisa Segal Spindle morphogenesis is regulated by cyclin-dependent kinases and monitored by checkpoint pathways to accurately coordinate chromosomal segregation with other events in the cell cycle. We have previously dissected the contribution...

...Chun Y. Gao; Mary Ann Stepp; Robert Fariss; Peggy Zelenka Recent studies have shown that Cdk5, a member of the cyclin-dependent-kinase family, regulates adhesion and migration in a mouse corneal epithelial cell line. Here, we extend these findings to corneal wound healing in vivo and examine...

...Tatiana García-Muse; Gero Steinberg; José Pérez-Martín Pathogenesis, morphogenesis and cell cycle are connected in the fungal pathogen Ustilago maydis . Here we report the characterization of the catalytic subunit of the cyclin-dependent kinase, encoded by the gene cdk1 , and the two B-type...

...Anabelle Decottignies; Patrick Zarzov; Paul Nurse We investigated the in vivo localisation of fission yeast cyclin-dependent kinase cdc2p during mitosis and meiosis. Fusion to yellow fluorescent protein (YFP) revealed that cdc2-YFP is present in the cytoplasm at all stages of the cell cycle...

... associated with yeast replication origins in cyclin-dependent kinase (cdk) mutants, and of the behaviour of the Mcm proteins in the presence of protein kinase inhibitors in mammalian cells ( Coverley et al., 1998 ; Dahmann et al., 1995 ; Piatti et al., 1996 ), suggest that phosphorylation events...

...Philipp Kaldis; Zachary W. Pitluk; I. Amy Bany; Deborah A. Enke; Marisa Wagner; Edward Winter; Mark J. Solomon ABSTRACT Eukaryotic cell cycles are controlled by the activities of cyclin-dependent kinases (cdks). The major cdk in budding yeast, Saccharomyces cerevisiae , is Cdc28p. Activation...

...Jean-Bernard Lazaro; Magali Kitzmann; Marie-Alix Poul; Marie Vandromme; Ned J. C. Lamb; Anne Fernandez ABSTRACT We have examined the expression, activity and localization of cyclin dependent kinase 5 (cdk5), during myogenesis. Cdk5 protein was found expressed in adult mouse muscle. In murine C2...

... kinase activity is required to shut off already activated cyclin-dependent kinase complexes or to protect a sub-population of a specific cdk complex from being activated. The assembly of the contractile structures necessary for cytokinesis and the stabilisation of polar microtubules might therefore...

... checkpoint cyclin-dependent kinase mitosis In the past few years, significant advances have been made in understanding the molecular mechanism controlling the pro-gression of the eukaryotic cell cycle ( Murray and Hunt, 1993 ). Central components of this mechanism are the cyclin-dependent protein...

..., and that the activation of p40 MO15 may require phosphorylation on T176. * Author for correspondence 11 05 1994 10 06 1994 © 1994 by Company of Biologists 1994 CAK cell cycle cyclin-dependent kinase Cyclins and cyclin-dependent kinases (CDKs) are key regulators of the eukaryotic...

...-mosomal translocation, DNA amplification and retroviral integration. Cyclins DI, D2 and D3 preferentially associate with two closely related members of the cyclin-dependent kinase family, Cdk4 and Cdk6 and the various complexes are each capable of phosphorylating the retinoblastoma gene product (pRb...

... subjected to SDS-PAGE or washed once more in kinase assay buffer ( Krek and Nigg, 1991 ) and used for histone H1 kinase assays. * Author for correspondence 31 05 1993 06 06 1993 © 1993 by Company of Biologists 1993 cyclin cdk cyclin-dependent kinase complex formation...