...-1-1/ Highlighted Article: A S. pombe checkpoint-independent pathway delays activation of the mitotic CDK after UV irradiation through selective translational downregulation of the mitotic cyclin Cdc13. G2/M UV Checkpoint Cyclin B Selective translation Helse Sør-Øst...

... and Myt1 kinase, resulting in activation of cyclin-B–Cdk1, which then induces meiotic G2/M transition. Here, we show that cyclin-B–Cdk1 is partially activated after subthreshold hormonal stimuli, but this triggers negative feedback, resulting in dephosphorylation of Akt sites on Cdc25 and Myt1, thereby...

... of eukaryotic pathways. Although Giardia has many cell cycle regulatory components, its genome lacks all anaphase-promoting complex (APC) components. In the present study, we show that a single mitotic cyclin in Giardia is essential for progression into mitosis. Strikingly, Giardia cyclin B lacks the conserved...

...Renata Basto; Fanni Gergely; Viji M. Draviam; Hiroyuki Ohkura; Kathryn Liley; Jordan W. Raff During mitosis, cyclin B is extremely dynamic and although it is concentrated at the centrosomes and spindle microtubules (MTs) in organisms ranging from yeast to humans, the mechanisms that determine its...

... of dividing cells in lateral roots compared with control plants (Bell et al.,1993). * Author for correspondence (e-mail: [email protected]) 31 10 2002 © The Company of Biologists Limited 2003 2003 Cyclin B Mitosis Checkpoint Plant development Progress through...

... in the mAb effect. Both developing starfish and Xenopus oocytes were injected with two different α-tubulin C terminus antibodies. The injected antibodies blocked cell entry into mitosis through specific inhibition of cyclin B synthesis. The antibody effect was independent of the presence or absence...

...Ariane Abrieu; Marcel Dorée; Daniel Fisher ABSTRACT Throughout oocyte maturation, and subsequently during the first mitotic cell cycle, the MAP kinase cascade and cyclin-B–Cdc2 kinase are associated with the control of cell cycle progression. Many roles have been directly or indirectly attributed...

... and of Cdc2 and cyclin B (the catalytic and regulatory subunits of MPF) were followed by dissection of intact eggs following freezing and in cultured fragments separated by ligation. Cdc2 was found to be distributed evenly throughout the egg cytoplasm. Loss of phosphorylated (inactive) forms of Cdc2 coincided...

... by maturation promoting factor (MPF: p34 cdc2 /cyclin B complex), little is known about how p34 cdc2 kinase controls microtubule networks. We provide evidence of the direct association of the p34 cdc2 /cyclin B complex with microtubules in starfish oocytes. Anti-cyclin B staining of detergent-treated oocytes...

... that of interphasic extracts, while PCM accumulation in mitotic extracts seemed to be similar to that in interphasic extracts. The increase in microtubule nucleation from KCl-treated centrosomes was strikingly enhanced by the addition of purified p34 cdc2 /cyclin B complex to interphasic extracts, but not by MAP...