... the early phases of the nitrogen starvation response. This excessive TORC1 reactivation results in the silencing of the Ste11-Mei2 pathway and mating defects. Additionally, we found that pef1 genetically interacts with tsc1 and tsc2 for TORC1 regulation, and physically interacts with three cyclins, Clg1...

...Yang Ma; Guanxiong Yan; Xiaojie Han; Jing Zhang; Jie Xiong; Wei Miao ABSTRACT To investigate the mechanisms underlying initiation of the sexual cell cycle in eukaryotes, we have focused on cyclins and cyclin-dependent kinases (CDKs) in the well-studied model ciliate, Tetrahymena thermophila . We...

...Nikki A. Copeland; Heather E. Sercombe; Justin F. X. Ainscough; Dawn Coverley Initiation of mammalian DNA replication can be reconstituted from isolated G1-phase nuclei and cell extracts, supplemented with cyclin-dependent protein kinases (CDKs). Under these conditions, cyclin E supports pre...

...Reinhard Sigl; Cornelia Wandke; Veronika Rauch; Jane Kirk; Tim Hunt; Stephan Geley The anaphase-promoting complex/cyclosome (APC/C) is essential for progression through mitosis. At anaphase onset, the APC/C requires the activator protein CDC20 to target securin and cyclin B1 for proteasome...

...Adam Walker; Claire Acquaviva; Takahiro Matsusaka; Lars Koop; Jonathon Pines Ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase lies at the heart of the cell cycle. The APC/C targets mitotic cyclins for destruction in mitosis and G1 phase...

... is not due to a defect in the recruitment of the polarisome scaffold protein Spa2 or the actin cytoskeleton regulators Cdc42 and Cdc24 in the neck region. Previous results indicate a connection between Rot1 and the cyclin Clb2. In fact, overexpression of CLB2 is toxic when ROT1 is partially inactivated...

...Raïssa Eluère; Nicolas Offner; Isabelle Varlet; Olivia Motteux; Laurence Signon; André Picard; Eric Bailly; Marie-Noëlle Simon Orderly progression through the eukaryotic cell cycle is a complex process involving both regulation of cyclin dependent kinase activity and control of specific...

... is therefore first required after egg activation for polar body condensation and zygotic mitoses. Embryos containing pronounced anterior-posterior gradients of Gnu activity demonstrate that Gnu regulates mitotic activity by promoting cyclin B stability. Our gnuGFP-bcd3 ′ UTR vector provides a novel...

... in the context of clinical conditions — especially cancers — that arise from de-regulated cell proliferation in response to aberrant Hedgehog signalling activity. References Barnes, E. A., Kong, M., Ollendorff, V. and Donoghue, D. J. ( 2001 ). Patched1 interacts with cyclin B1 to regulate cell cycle...

...Marcel Dorée; Tim Hunt The idea that Cdc2 and cyclins play a key role in the control of the G2/M transition of the cell cycle came largely from genetic analysis of fission yeast and physiological studies of clam, frog, sea urchin and starfish eggs and oocytes. However, it took a long time...

...Bradley J. Schnackenberg; William F. Marzluff In somatic cells, cyclin E-cdk2 activity oscillates during the cell cycle and is required for the regulation of the G1/S transition. Cyclin E and its associated kinase activity remain constant throughout early sea urchin embryogenesis, consistent...

...Nicholas P. Edgington; Bruce Futcher The Saccharomyces cerevisiae cyclin-dependent kinase Cdc28 forms complexes with nine different cyclins to promote cell division. These nine cyclin-Cdc28 complexes have different roles, but share the same catalytic subunit; thus, it is not clear how substrate...

...Mary E. Miller; Frederick R. Cross ABSTRACT Cyclin-dependent kinase (CDK) activity is essential for eukaryotic cell cycle events. Multiple cyclins activate CDKs in all eukaryotes, but it is unclear whether multiple cyclins are really required for cell cycle progression. It has been argued...

...Juinn-Lin Liu; Michael D. Hebert; Ying Ye; Dennis J. Templeton; Hsing-Jien Kung; A. Gregory Matera ABSTRACT We have found that CDK2 and cyclin E, but not cyclin A, accumulates within Cajal bodies (CBs) in a cell cycle-dependent fashion. In the absence of cyclin E, CDK2 is not enriched in the CB...

...: Department of Pediatrics, University of Zurich, Zurich, Switzerland CH-8032 13 10 1999 30 11 1999 © 1999 by Company of Biologists 1999 G 1 phase Cyclin Adhesion Anchorage-independent growth Cytoskeleton Integrins are heterodimers consisting of noncovalently associated...

...Alberto Sánchez-Díaz; Isabel González; Manuel Arellano; Sergio Moreno ABSTRACT p25 rum1 and p40 SIC1 are specific inhibitors of p34 cdc2/CDC28 kinase complexes with B-type cyclins that play a central role in the regulation of the G 1 phase of the cell cycle. We show here that low levels...

...Doris Germain; Joy Hendley; Bruce Futcher ABSTRACT Cell cycle progression is mediated by waves of specific cyclin dependent kinases (CDKs) in all eukaryotes. Cyclins are degraded by the ubiquitin pathway of proteolysis. The recent identification of several components of the cyclin proteolysis...

... metazoan development. Now we can study how the crucial regulators of eukaryotic cell cycle progression, the various cyclin/cdk complexes (for a recent review see Nigg (1995) BioEssays 17, 471-480), are turned on or off during development. In Drosophila , this analysis is most advanced, in particular...

...S. Chevalier; A. Couturier; I. Chartrain; R. Le Guellec; C. Beckhelling; K. Le Guellec; M. Philippe; C. C. Ford ABSTRACT The capacity to initiate DNA replication appears during oocyte maturation in Xenopus . Initiation of S phase is driven by several components which include active cyclin/cdk...

...J. Creanor; J. M. Mitchison ABSTRACT The levels of the B cyclin p56 cdc13 and the phosphatase p80 cdc25 have been followed in selection-synchronised cultures of Schizosaccharomyces pombe wild-type and wee1 mutant cells. p56 cdc13 has also been followed in induction-synchronised cells of the mutant...