... new proteasomes are assembled. Proteasome Core particle Assembly Chaperone Cryo-electron microscopy National Institutes of Health http://dx.doi.org/10.13039/100000002 R01-GM144367 R01-GM135337 The proteasome is the most complex protease ever described...

... structural and biophysical studies have shed much light on the physical mechanism of ESCRT-mediated membrane remodeling and scission. ESCRT Membrane scission HIV Endosome Membrane biophysics In vitro reconstitution Giant unilamellar vesicle Cryo-electron microscopy Optical tweezers...

... is a long-standing question in cilia biology. The radial spoke has three regions – a spoke head, a bifurcated neck and a stalk. Although the neck and the stalk are asymmetric, twofold symmetry of the head has remained controversial. In this work we used single particle cryo-electron microscopy (cryo-EM...

... Cryo-electron microscopy Image reconstruction Microtubule motors Kinesin motors use energy supplied by ATP hydrolysis to move cargo along microtubules (MTs) or, in some cases, to depolymerise these cytoskeletal filaments. All have very similar motor domains and appear to follow the same cycle...

..., there is a lack of consensus on lamellipodia organisation stemming from the application of alternative procedures for ultrastructural visualisation of cytoskeleton networks. In this study, we show that cryo-electron microscopy of extracted cytoskeletons embedded in a thin layer of vitreous ice can reveal...