... new proteasomes are assembled. Proteasome Core particle Assembly Chaperone Cryo-electron microscopy National Institutes of Health http://dx.doi.org/10.13039/100000002 R01-GM144367 R01-GM135337 The proteasome is the most complex protease ever described...

... structural and biophysical studies have shed much light on the physical mechanism of ESCRT-mediated membrane remodeling and scission. ESCRT Membrane scission HIV Endosome Membrane biophysics In vitro reconstitution Giant unilamellar vesicle Cryo-electron microscopy Optical tweezers...

... is a long-standing question in cilia biology. The radial spoke has three regions – a spoke head, a bifurcated neck and a stalk. Although the neck and the stalk are asymmetric, twofold symmetry of the head has remained controversial. In this work we used single particle cryo-electron microscopy (cryo-EM...

... from the active site of the directly bound head via its central β-sheet to the tethered head. * Author for correspondence (e-mail: [email protected] ) 3 10 2007 © The Company of Biologists Limited 2007 2007 Cryo-electron microscopy Image reconstruction Microtubule...

..., there is a lack of consensus on lamellipodia organisation stemming from the application of alternative procedures for ultrastructural visualisation of cytoskeleton networks. In this study, we show that cryo-electron microscopy of extracted cytoskeletons embedded in a thin layer of vitreous ice can reveal...