... their apical domain to grow cilia, in coordination with the underlying actin cytoskeleton. Crumbs (Crb) proteins are multifaceted transmembrane apical polarity proteins known to recruit actin linkers and promote apical membrane growth. Here, we identify the homeolog Crb3.L as an important player...

...Ji-Hyun Hwang; Linh Thuong Vuong; Kwang-Wook Choi ABSTRACT Xeroderma Pigmentosum D (XPD, also known as ERCC2) is a multi-functional protein involved in transcription, DNA repair and chromosome segregation. In Drosophila , Xpd interacts with Crumbs (Crb) and Galla to regulate mitosis during...

...Milena Pellikka; Ulrich Tepass ABSTRACT Mutations in human crumbs 1 ( CRB1 ) are a major cause of retinal diseases that lead to blindness. CRB1 is a transmembrane protein found in the inner segment of photoreceptor cells (PRCs) and the apical membrane of Müller glia. The function...

...Craig I. Campbell; Payman Samavarchi-Tehrani; Miriam Barrios-Rodiles; Alessandro Datti; Anne-Claude Gingras; Jeffrey L. Wrana ABSTRACT The Crumbs complex is an important determinant of epithelial apical-basal polarity that functions in regulation of tight junctions, resistance to epithelial...

...Ya-Huei Lin; Heather Currinn; Shirin Meher Pocha; Alice Rothnie; Thomas Wassmer; Elisabeth Knust ABSTRACT Maintenance of epithelial polarity depends on the correct localization and levels of polarity determinants. The evolutionarily conserved transmembrane protein Crumbs is crucial for the size...

.... The formation and establishment of epithelial cell polarity is mediated by the cooperation of polarity protein complexes, namely the Crumbs, partitioning defective (Par) and Scribble complexes, with Rho family GTPases, including RhoA, Rac1 and Cdc42. The activation of different GTPases triggers distinct...

...Annalisa Letizia; Sara Ricardo; Bernard Moussian; Nicolás Martín; Marta Llimargas Summary Regulated cell shape changes in epithelial cells, which contribute to most organs and tissues, are at the basis of morphogenesis. Crumbs (Crb) is an essential apical determinant controlling epithelial...

... Crumbs (Crb) organises an apical membrane-associated protein complex that controls photoreceptor morphogenesis. Expression of the small cytoplasmic domain of Crb in crb mutant photoreceptor cells (PRCs) rescues the crb mutant phenotype to the same extent as the full-length protein. Here, we show...

...François J.-M. Chartier; Émilie J.-L. Hardy; Patrick Laprise Drosophila Crumbs (Crb) and its mammalian ortholog CRB3 control epithelial polarity through poorly understood molecular mechanisms. Elucidating these mechanisms is crucial, because the physiology of epithelia largely depends...

... to morphogenesis are two important questions in development. Here, we approach these issues by investigating the role of the apical determinant protein Crumbs (Crb) during the morphogenesis of the embryonic Drosophila tracheal system. Crb accumulates differentially throughout tracheal development and is required...

.... Roughest and the cadherin-based zonula adherens (ZA) are interdependent and both are modulated by the apical polarity determinant, Crumbs. Here we describe a novel relationship between the Crumbs partner β Heavy -spectrin (β H ), the ZA and Roughest. Ectopic expression of the C-terminal segment 33 of β H...

... for the establishment of tubule cell polarity, the key apical determinant, Crumbs, is required for cell polarity in the tubules only from the time when morphogenetic movements start. Strikingly, if these movements are stalled, polarity persists in the absence of Crumbs. Similar rescue of the ectodermal phenotype...

...Nana Bit-Avragim; Nicole Hellwig; Franziska Rudolph; Chantilly Munson; Didier Y. S. Stainier; Salim Abdelilah-Seyfried The zebrafish MAGUK protein Nagie oko is a member of the evolutionarily conserved Crumbs protein complex and functions as a scaffolding protein involved in the stabilization...

... morphogenesis. We previously implicated the small GTPase Drac1 in the assembly of an actomyosin contractile apparatus, contributing to cell shape change in the epidermis during dorsal closure. We now present evidence that Drac1 and Crumbs, a determinant of epithelial polarity, are involved in setting up...